Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers

Authors

  • Jumailatus Solihah Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281, Indonesia http://orcid.org/0000-0001-8225-9654
  • Isma Kurniatanty Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281, Indonesia http://orcid.org/0000-0002-3322-3919
  • Dyah Subositi Medicinal Plant dan Traditional Medicine Research and Development Center, National Institute of Health Research and Development, Indonesia http://orcid.org/0000-0003-1596-5625
  • Anshary Maruzy Medicinal Plant dan Traditional Medicine Research and Development Center, National Institute of Health Research and Development, Indonesia http://orcid.org/0000-0002-2638-2276
  • Ika Nugraheny Ari Martiwi Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281, Indonesia
  • Erny Qurrotul Ainy Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281, Indonesia
  • Khoirul Anam Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281, Indonesia
  • Aslikh Lana Dina Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281, Indonesia

DOI:

https://doi.org/10.15575/biodjati.v6i2.14553

Keywords:

enetic characterisation, Sida rhombifolia, SRAP

Abstract

Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplified Polymorphism (SRAP) Markers. The genomic DNA were extracted from leaf samples and then were characterized by using the SRAP marker system according to Li and Quiros (2001). Nine pairs of SRAP primer resulted high polymorphic bands and were used in the genetic profiling. The data analysis was performed using GenAlEx to calculate genetic distance, Principal coordinate analysis, and Analysis of Molecular Variance (AMOVA), also using POPGENE to assess genetic diversity (Hs and Ht) and Nm to predict gene flow among populations. The coordinate analysis showed that the accessions originated from ethnicities along Wallacean line tend to differ genetically from most other locations. However, the results of analysis of molecular variance suggested that there were only slight differences (0.1%) found between ethnicities, while most genetic variances (99.9%) were found mostly among accessions within populations. The results suggested that there was an extensive genetic flow and plant spreading among Sida rhombifolia plant populations, resulting more homogenous genetic characters among most populations, while high diversity within population. The calculation of the number of migration (Nm = 1.7341) confirmed that the high rate of gene flow had occurred between populations.

Author Biographies

Jumailatus Solihah, Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281

Department of Biology

Isma Kurniatanty, Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281

Department of Biology

Ika Nugraheny Ari Martiwi, Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281

Department of Biology

Erny Qurrotul Ainy, Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Kalijaga, Yogyakarta, Jl. Marsda Adisucipto Yogyakarta, 55281

Department of Biology

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Published

29-11-2021

How to Cite

Solihah, J., Kurniatanty, I., Subositi, D., Maruzy, A., Martiwi, I. N. A., Ainy, E. Q., … Dina, A. L. (2021). Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers. Jurnal Biodjati, 6(2), 203–212. https://doi.org/10.15575/biodjati.v6i2.14553

Issue

Vol. 6 No. 2 (2021): November

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Articles

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