Optimalisasi Kultur Daphnia yang Berperan sebagai Hewan Uji dalam Ekotoksikologi

Authors

  • Hertien Koosbandiah Surtikanti Program Studi Biologi, Departemen Pendidikan Biologi, FPMIPA UPI, Indonesia
  • Rahardian Juansah Departemen Pendidikan Biologi, FPMIPA UPI, Jl. Dr. Setiabudhi no 229 Bandung
  • Diah Frisda Departemen Pendidikan Biologi, FPMIPA UPI, Jl. Dr. Setiabudhi no 229 Bandung

DOI:

https://doi.org/10.15575/biodjati.v2i2.1571

Keywords:

Neonate, Daphnia, kultur, medium

Abstract

Daphnia (udang-udangan) merupakan hewan standar Internasional yang layak dan sering digunakan dalam pengujian kualitas air. Salah satu persyaratan sebagai hewan uji diantaranya adalah, bahwa hewan tersebut dapat dikultur dalam kondisi laboratorium. Oleh sebab itu diperlukan studi awal tentang kultur Daphnia di Laboratorium Riset Lingkungan di FPMIPA, UPI. Studi ini bertujuan untuk (1) menentukan jumlah neonate (umur < 24 jam) yang dihasilkan oleh satu gravid female Daphnia dalam 250 ml medium dan (2) menentukan tingkat kesintasan 10 neonate selama 3 hari dalam 3 medium (10 mL) yang berbeda (air tawar buatan, air sumur dan air PDAM). Experimen ini dilakukan masing-masing dengan 5 kali ulangan. Hasil yang diperoleh bahwa rata-rata jumlah neonate yang dihasilkan dari satu induk Daphnia pada hari ke 2 dalam medium air buatan, air sumur dan air PDAM berturut-turut adalah 14; 13; dan 11. Sedangkan rata-rata jumlah neonate yang mampu bertahan dalam medium air buatan, air sumur dan air PDAM adalah 8.5-10 ekor. Hasil studi ini menunjukkan bahwa Daphnia dapat dikultur di laboratorium dan neonate yang dihasilkan mengalami tingkat kesintasan lebih dari 80% hingga hari ke 3 dengan menggunakan ketiga medium yang berbeda. Sehingga neonate layak digunakan dalam uji hayati metode statis tanpa pakan dan oksigen.

Author Biographies

Hertien Koosbandiah Surtikanti, Program Studi Biologi, Departemen Pendidikan Biologi, FPMIPA UPI

Departemen Pendidikan Biologi, FPMIPA UPI, Jl. Dr. Setiabudhi no 229 Bandung

Rahardian Juansah, Departemen Pendidikan Biologi, FPMIPA UPI, Jl. Dr. Setiabudhi no 229 Bandung

Departemen Pendidikan Biologi, FPMIPA UPI, Jl. Dr. Setiabudhi no 229 Bandung

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Published

30-11-2017

How to Cite

Surtikanti, H. K., Juansah, R., & Frisda, D. (2017). Optimalisasi Kultur Daphnia yang Berperan sebagai Hewan Uji dalam Ekotoksikologi. Jurnal Biodjati, 2(2), 83–88. https://doi.org/10.15575/biodjati.v2i2.1571

Issue

Vol. 2 No. 2 (2017): November

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