Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes

Authors

  • Kahar Muzakhar Department of Biology, Faculty Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan, Jember, Indonesia 68121, Indonesia
  • Rudju Winarsa Department of Biology, Faculty Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan, Jember, Indonesia 68121, Indonesia

DOI:

https://doi.org/10.15575/biodjati.v4i1.4411

Keywords:

α-L-Rhamnosidase, Aspergillus niger, coffee pulp wastes, solid-state fermentation

Abstract

 An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C. 

Author Biography

Kahar Muzakhar, Department of Biology, Faculty Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan, Jember, Indonesia 68121

Jurusan Biologi, FMA Universitas Jember

References

Birgisson, H., Wheat, J. O., Hreggvidsson, G. O., Kristjansson, J. K. & Mattiasson, B. (2007). Immobilization of a Recombi-nant Escherichia coli Producing a Ther-mostable α-L-rhamnosidase: Creation of a Bioreactor for Hydrolyses of Nar-ingin. Enzyme and Microbial Technolo-gy, 40(5), 1181–1187.

Bokkenheuser, V. D., Shackleton, C. H. & Winter, J. (1987). Hydrolysis of Dietary Flavonoid Glycosides by Strains of In-testinal Bacteroides from Humans. Bio-chemical Journal, 248(3), 953–956.

Caldini, C., Bonomi, F., Pifferi, P. G., Lanza-rini, G. & Galante, Y. M. (1994). Kinetic and Immobilization Studies on Fungal Glycosidases for Aroma Enhancement in Wine. Enzyme and Microbial Tech-nology, 16(4), 286–291.

Ding, G., Pei, J., Ge, L., Chen, A., Wang, Z., Xiao, W. & Tang, F. (2017). Enhancing the Thermostability of α-L-rhamnosi-dase from Aspergillus terreus and the Enzymatic Conversion of Rutin to Iso-quercitrin by Adding Sorbitol. BMC Bi-otechnology, 17(1), 1–10.

Ellaiah, P., Prabhakar, T., Ramakrishna, B., Thaer Taleb, A. & Adinarayana, K. (2004). Production of Lipase by Immo-bilized Cells of Aspergillus niger. Pro-cess Biochemistry, 39(5), 525–528.

Gastón Orrillo, A., Ledesma, P., Delgado, O. D., Spagna, G. & Breccia, J. D. (2007). Cold-Active α-L-rhamnosidase from Psychrotolerant Bacteria Isolated from a Sub-Antarctic Ecosystem. Enzyme and Microbial Technology, 40(2), 236–241.

Habelt, K., & Pittner, F. (1983). A Rapid Method for the Determination of Nar-ingin, Prunin and Naringenin Applied to the Assay of Naringinase. Analytical Biochemistry, 134(2), 393–397.

Hatzinikolaou, D. G., Macris, J. B., Chris-takopoulos, P., Kekos, D., Kolisis, F. N. & Fountoukidis, G. (1996). Production and Partial Characterisation of Extra-cellular Lipase from Aspergillus niger. Biotechnology Letters, 18(5), 547–552.

Lúcia, F. P. A., da Silva, M. F., de Moura, R. B., Ribeiro Sales, M. & de Macedo, G. R. (2011). Cellulase and Xylanase Pro-duction by Aspergillus species. Annals of Microbiology, 61(4), 917–924.

Muzakhar, K., Masruroh, Siswoyo, Winar-sa, R. & Sutoyo. (2017). Sugar-Rich Hydrolysates of Palm Oil Empty Fruit Bunch Production Through Two Step Solid State Fermentations and its Con-version to Ethanol. Advanced Science Letters, 23(3), 2533–2535.

Muzakhar, K., Sutoyo, S. & Saragih, A. B. (2015). Phosphate Solubilizing Bacteria Adaptive to Vinasse. Journal of Math-ematical and Fundamental Sciences, 47(2), 219–225.

Narasimha, G., Reddy, B. R., Dileepkumar, K., Ramanjaneyulu, G., Sridevi, A. & Devi, P. S. (2015). Saccharification of Pretreated Sawdust by Aspergillus niger Cellulase. 3 Biotech, 5(6), 883–892.

Prakasham, R. S., Subba Rao, C., Sreenivas Rao, R. & Sarma, P. N. (2007). Enhance-ment of Acid Amylase Production by an Isolated Aspergillus awamori. Journal of Applied Microbiology, 102(1), 204– 211.

Reddy, G. P. K., Narasimha, G., Kumar, K. D., Ramanjaneyulu, G., Ramya, A., Shanti, B. S. & Rajasekhar, B. (2015). Cellu-lase Production by Aspergillus niger on Different Natural Lignocellulosic Sub-strates. International Journal of Cur-rent Microbiology and Appied Sciences, 4(4), 835–845.

Vila-Real, H., Alfaia, A. J., Bronze, M. R., Calado, A. R. T. & Ribeiro, M. H. L. (2011). Enzymatic Synthesis of the Fla-vone Glucosides, Prunin and Isoquerce-tin, and the Aglycones, Naringenin and Quercetin, with Selective α -L-rham-nosidase and β -D-glucosidase Activi-ties of Naringinase. Enzyme Research, 2011(1).

Wang, H., Qian, S., Lu, M., Zhang, C., Jin, F. & Yu, H. (2005). Purification and Char-acterization of Dioscin-α-L-rhamnosi-dase from Pig Liver. Chemical & Phar-maceutical Bulletin, 53(8), 911–914.

Yadav, S. (2018). Prunin Production From Orange Peel Naringin Hydrolyzed By a-L-rhamnosidase from Aspergillus fla-vipus MTCC- 4644. International Jour-nal of Trend in Scientific Research and Development, 2(5), 1271–1278.

Yadav, V., Yadav, P. K., Yadav, S. & Yadav, K. D. S. (2010). α-L-Rhamnosidase: A review. Process Biochemistry, 45(8), 1226–1235.

Yadav, V., Yadav, S., Yadava, S. & Yadav, K. D. S. (2011). α-L-Rhamnosidase from Aspergillus flavus MTCC-9606 Isolated from Lemon Fruit Peel. International Journal of Food Science and Technolo-gy, 46(2), 350–357.

Downloads

Published

28-05-2019

How to Cite

Muzakhar, K., & Winarsa, R. (2019). Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes. Jurnal Biodjati, 4(1), 105–111. https://doi.org/10.15575/biodjati.v4i1.4411

Issue

Vol. 4 No. 1 (2019): May

Section

Articles

Citation Check

License

Copyright and Attribution:

Copyright of published in Jurnal Biodjati is held by the journal under Creative Commons Attribution (CC-BY-NC-ND) copyright. The journal lets others distribute and copy the article, create extracts, abstracts, and other revised versions, adaptations or derivative works of or from an article (such as an tranlation), include in collective works (such as an anrhology), text or data mine the article, as long as they credit the author(s), do not represent the author as endorsing their adaptation of the article and do not modify the article in such a way as to damage the author's honor or reputation.

Permissions:

Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate of one of the authors.

Ethical matters:

Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the authors own must have had the correct authorization for this exportation.