Isolation and Characterization of Indole-3-Acetic Acid Producing Bacteria from Cow Urine
DOI:
https://doi.org/10.15575/biodjati.v4i1.4481Keywords:
bacteria, cow urine, green bean, indole-3-acetic acidAbstract
Â
 Cow urine contains urea as nitrogen source, therefore it can be expected to isolate the beneficial bacteria for plants, for example indole-3-acetic acid (IAA) or auxin producing bacteria. The objective of research was to obtain IAA producing bacteria from cow urine, to characterize bacterial isolate, and to measure its ability to stimulate the growth of green bean seedlings (Vigna radiata). The methods used in this study were collecting urine from cow cattle, obtaining IAA-pro-ducing bacteria from urine, measuring IAA using Salkowski method, and applying selected bacterial supernatants on green bean seedling plants. The number of IAA producing bacteria that was successfully purified was 18 isolates. There are five isolates, namely US 5, BS1, BS 2, BS 4 and BS 5 which have the ability to solubelize phosphate on Pikovskaya agar. The five isolates were also able to fix free nitro-gen on N Free media and did not show hypersensitivity on tobacco leaves. The results of the growth of isolates in blood agar showed positive for US 5 and BS 2 as beta hemolysin producers. Further-more, isolate BS 4 was chosen to produce exogenous IAA quantita-tively. Isolate BS 4 produced IAA 6.364 ppm at the 45 h incubation at stationary phase. The use of BS 4 supernatant on green bean seed-lings showed an effect on plant height and lateral root length better than control (without treatment) on 6 days after planting. Morpho-logical characteristic of isolate BS 4 was rod shape, Gram positive, endospore producing, aerobic, and had similarity with genus Bacillus
References
Adijaya, I. N., Sudaratmaja, I. G. A. K., Mahaputra, I. K, Trisnawati, N. W., Suharyanto, Guntoro, S., Rinaldi, J., Elizabeth, D. A. A., Priningsih, P. Y. & Rachim, A. (2008). Prima Tani LKDRIK Desa Sanggalangit, Kec. Gerogak, Kab. Buleleng, Bali. Denpasar: Balai Peng-kajian Teknologi Pertanian.
Anandam, R., Premalatha, N., Jen, H. J., Weon, H. Y., Kwon, S. W., Krisnamoorthy, R., Gandhi, P. I., Kim, Y. K. & Gopal, N. O. (2015). Cultivable Bacterial Diversity and Early Plant Growth Promotion by the Traditional Organic Formulations Prepared using Organic Waste Materi-als. Int. J. Recycl. Org. Waste. Agricult, 4, 279-289.
Ariyanti, D., Salasia, S. I. O. & Tato, S. (2011). Characterization of Haemolysin of Staphylococcus aureus Isolated from Food of Animal Origin. Indones. J. Bio-technol, 16(1), 32-37.
Astriani, M., Mubarik, N. R. & Tjahjolek-sono, A. (2016). Selection of Bacteria Producing Indole-3-acetic Acid and Its Application on Oil Palm Seedlings (Elaeis guineensis Jacq.). Malay. J. Mi-crobiol, 12(2), 147-154.
Benjamins, R & Scheres, B. (2008). Auxin: the Looping Star in Plant Development. Ann. Rev. Plant. Biol, 59, 443-465.
Compant, S., Duffy, B., Nowak, J., Clement, C. & Barka, E. A. (2005). Use of Plant Growth Promoting Bacteria for Bio-control of Plant Diseases: Principles, Mechanisms of Action and Future Pros-pects. Appl. Environ. Microbiol., 71, 4951-4959.
Duca, D., Lorv, J., Patten, C. L., Rose, D. & Glick, B. R. (2014). Indole-3-acetic Acid in Plant Microbe Interaction. An-tonie Van Leeuwenhoek, 106, 85-125.
Harca, N. N., Mubarik, N. R. & Wahyudi, A. T. (2014). Isolation and Identification of Nitrogen-fixing and Indole Acetic Acid Producing Bacteria from Oil Palm Plan-tation in Jambi, Indonesia. J. Int. Envi-ron. Appl. Sci, 9(4), 546-553.
Garuda, Triadiati & Mubarik, N. R. (2014). Corn Growth and Yield Improvement using Biofertilizer Based on Plant Growth Promoting Bacteria in Acid Soil. J. Int. Environ. Appl. Sci, 9(4), 569-576.
Kafrawi, Baharuddin, Sengin, E. L. & Rosma-na, A. (2014). Screening of Free-living Indole Acetic Acid Producing Rhizo-bacteria from Shallot Rhizospheres in the Island of Sulawesi. Int. J. Sci. Tech-nol. Res, 3(2), 118-121.
Patten, C. L. & Glick, B.R. (2002). Role of Pseudomonas putida Indole Ace-tic Acid in Development of the Root Plant System. Appl Environ Microbial. 68(8):3795-3801.
Shah, C. P., Patel, D. M., Dhami, P. D., Kakadia, J., Bhausar, D., Vachani, U. D., Trivedi, M. N. & Joshi, V. J. (2011). In Vitro Screening of Antibacterial Ac-tivity of Cow Urine against Pathogenic Human Bacterial Strains. Int. J. Curr. Pharmaceut. Res, 3(2), 91-92
Spaepen, S., Varderleyden, J. & Remans, R. (2007). Indole-3-Acetic Acid in micro-bial and Microorganism-plant Signal-ing. FEMS Microbiol. Rev, 10, 1-24.
Torres-Rubio, M. G., Valencia-Plata, S. A., Bernall-Castilo, J. & Martinez-Nicto, P. (2000). Isolation of Enterobacteria, Az-otobacteria sp. and Pseudomonas sp., Producers of Indole-3-acetic-acid and Siderosphores from Colombian Rice Rhizosphere. Rev. Lat. Microbiol, 42, 171-176.
Wahyudi, A. T., Meliah, S. & Nawangsih, A. A. (2011). Xanthomonas oryzae pv. ory-zae Bakteri Penyebab Hawar Daun pada Padi: Isolasi, Karakterisasi dan Telaah Mutagenesis dengan Transposon. Ma-kara Sains, 15(1), 89-96.
Downloads
Published
How to Cite
Issue
Section
Citation Check
License
Copyright and Attribution:
Copyright of published in Jurnal Biodjati is held by the journal under Creative Commons Attribution (CC-BY-NC-ND) copyright. The journal lets others distribute and copy the article, create extracts, abstracts, and other revised versions, adaptations or derivative works of or from an article (such as an tranlation), include in collective works (such as an anrhology), text or data mine the article, as long as they credit the author(s), do not represent the author as endorsing their adaptation of the article and do not modify the article in such a way as to damage the author's honor or reputation.
Permissions:
Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate of one of the authors.
Ethical matters:
Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the authors own must have had the correct authorization for this exportation.
