The Effect of Coffee Fruit Skin Extract on Sperm Characteristics And Testicular of Mice With Ethanol-Induced

Authors

  • Haris Setiawan Laboratory of Animal Structure and Physiology, Program Study of Biology, Faculty of Applied Science and Technology, Universitas Ahmad Dahlan, Jl. Ringroad Selatan, Tama-nan, Kec. Banguntapan, Bantul, DIY Yogyakarta, (0274) 511830, Indonesia
  • Rita Maliza Laboratory of Animal Structure and Physiology, Program Study of Biology, Faculty of Applied Science and Technology, Universitas Ahmad Dahlan, Jl. Ringroad Selatan, Tama-nan, Kec. Banguntapan, Bantul, DIY Yogyakarta, (0274) 511830
  • Syaiful Adam Maulana Laboratory of Animal Structure and Physiology, Program Study of Biology, Faculty of Applied Science and Technology, Universitas Ahmad Dahlan, Jl. Ringroad Selatan, Tama-nan, Kec. Banguntapan, Bantul, DIY Yogyakarta, (0274) 511830, Indonesia
  • Muhammad Ilham Hisbullah Laboratory of Animal Structure and Physiology, Program Study of Biology, Faculty of Applied Science and Technology, Universitas Ahmad Dahlan, Jl. Ringroad Selatan, Tama-nan, Kec. Banguntapan, Bantul, DIY Yogyakarta, (0274) 511830, Indonesia

DOI:

https://doi.org/10.15575/biodjati.v5i2.9280

Keywords:

coffee skin extract, ethanol, reproductive system, sperm characteristics, testicular histology

Abstract

Coffee fruit skin contains antioxidant compounds that can repair damaged tissues, especially those of reproductive or-gans. This study was designed to assess to what extent the metha-nolic extract of coffee fruit skin affected sperm characteristics and testicular histology in mice receiving 15% ethanol for 15 days. It used 25 male mice aged four months and employed a completely randomized design with two controls (K) and three treatments (P), namely K1 (without ethanol), K2 (15% ethanol), P1 (15% ethanol and the methanolic extract of coffee fruit skin, or MECS, at 125 mg/ kg BW), P2 (15% ethanol and MECS 250 mg/kg BW), and P3 (15% ethanol and MECS 500 mg/kg BW). These treatments were administered orally with a 1 mL disposable syringe for 15 days. The parameters observed were sperm characteristics (viability, morphology, and sperm count) and the microscopic structure of the testicles (lu-men area, diameter, and area of seminiferous tubules). These data were then analyzed using the one-way ANOVA, continued with the LSD and Duncan’s tests (P <0.05). The results showed that P2 (250 mg/kg BW) substantially improved sperm count (86.5x105±1.73 cells/ mL), sperm viability (87.26±0.05%), and the proportion of normal sperm morphology (93.33±0.004%) of mice with 15% ethanol-in-duced sperm damages. Testicular histology also confirmed improve-ments in spermatogenic cells, as evident from the lumen, diameter, and area seminiferous tubules after receiving P1 and P2. In conclu-sion, the optimum dose of the coffee skin extract for improving sperm quality and microscopic structures of mice testicles is 250 mg/kg BW. 

References

Adaramoye, O. A. & Akanni, O. O. (2015). Protective Effects of Artocarpus altilis (Moraceae) on Cadmium-induced Changes in Sperm Characteristics and Testicular Oxidative Damage in Rats. Andralogia, 48(2), 152-163.

Aitken, R. J., Jones, K. T. & Robertson, S. A. (2012). Reactive Oxygen Species and Sperm Function In Sickness and In Health. Journal of Andrology, 33(6): 1096–1106.

Akang, E., Oremosu, A., Dosumu, O. & Eji-wunmi, A. (2011). Telfairia ocidentalis, a Prophylactic Medicine for Alcohol’s Damaging Effect on the Testis. Mace-donian Journal of Medicinal Sciences, 4(4), 380-387.

Anzila, I., W M, A. P., Soewondo, A. & Ra-hayu, S. (2017). Pengaruh Ekstrak Eth-anol Kemangi (Ocimum canum Sims.) terhadap Struktur Histologi Testis Mencit (Mus musculus) Jantan. Biotropika: Journal of Tropical Biology, 5(1), 22-26.

Ayucitra, A., Indraswati, N., Mulyandasari, V., Dengi, Y. K., Francisco, G. & Yud-ha, A. (2011). Potensi Senyawa Feno-lik Bahan Alam Sebagai Antioksidan Alami Minyak Goreng Nabati. Jurnal Widya Teknik, 10(1), 1-10.

Campbell, N. A. & Reece, J. B. (2010). Biologi Jilid 3 Edisi ke 8. Jakarta: Erlangga.

Çeribaşi, A. O., Türk, G., Sönmez, M., Sak-in, F. & Ateşşahin, A. (2010). Toxic Effect of Cyclophosphamide on Sperm Morphology, Testicular Histology and Blood Oxidant-Antioxidant Balance, and Protective Roles of Lycopene and Ellagic Acid. Basic & Clinical Pharma-cology & Toxicology, 107(3), 730–736.

Cohen, H. K., Kruglick, P. L. M. A. & Gonza-lez, M. D. C. (2003). Effect of Maternal and Paternal Alcohol Consumption on Success Rates of In Vitro Fertilization and Gamete Intrafallopian Transfer. Fertility and Sterility, 79(2), 330-339.

Condorelli, R. A., Calogero, A. E., Vicari, E. & La Vignera, S. (2015). Chronic Con-sumption of Alcohol and Sperm Param-eters: Our Experience and The Main Evidences. Andrologia, 47, 368–379.

De Souza, B. R., Mathias, L. S,. De Souza, T. L. M. & Camargo, I. C. C. (2017). Histopathological and Morphometric Evaluation in the Testis and Epididymis of Adult Rats Submitted to a Recovery Period After Treatment with Anabolic Steroid, Alcohol, and/or Nicotine. Jour-nal of Interdisciplinary Histopathology, 0(0), 1-7.

Dosumu, O. O., Osinubi, A. A. A. & Duru, D. I. O. (2014). Alcohol Induced Testicular Damage: Can Abstinence Equal Recovery?. Middle East Fertility Socety Journal.

Dungira, S. G., Katja, D. G. & Kamu, V. S. (2012). Aktivitas Antioksidan Eks-trak Fenolik dari Kulit Buah Manggis (Garcinia mangostana L.). Jurnal Mipa Unsrat Online, 1(1):11-15.

Esquivel, P. & Jimenez, V. M. (2012). Functional Properties of Coffe by-products. Food Research International, 46(2), 488-495.

Guyton & Hall. (2016). Textbook of Medical Physiology Thirteenth Edition. Missis-sippi: University of Mississippi Medi-cal Center Jackson.

Hardiningtyas, S. D., Purwaningsih, S. & Handharyani, E. 2014. Aktivitas Anti-oksidan dan Efek Hepatoprotektif Daun Bakau api-api putih. Jurnal Pengolahan Hasil Perikanan Indonesia, 17(1), 80-91.

Hayati, F. (2010). Pengaruh Pemberian Ek-strak Pasak Bumi (Eurycoma longifolia J.) Terstandar Terhadap Kualitas Sper-matozoa, Profil Testis, dan Hormon Tes-tosteron Tikus Putih Jantan. Disertasi. Yogyakarta: Universitas Gadjah Mada.

Jamalan, M., Ghaffari, M. A., Hoseinzadeh, P., Hashemitabar, M. & Zeinali, M. (2015). Human Sperm Quality and Metal Toxi-cants: Protective Effects of some Flavo-noids on Male Reproductive Function. Int J Fertil Steril, 9(3): 215-222.

La Maestra, S., De Flora, S. & Micale, R. T. (2015). Effect of Cigarette Smoke on DNA Damage, Oxidative Stress, and Morphological Alterations in Mouse Testis and Spermatozoa. International Journal of Hygiene and Environmental Health, 218(1), 117–122.

Lara, L. V., Pasqualotto, E. B., Junior, E. B., Braga, D. P. A. F., Salvador, M. & Pasqualotto, F. F. (2008). Flavonoids May Increase Semen Quality in Infer-tile Man with Oligospermia?. American Society for Reproductive Medicine, 90, 190-191.

Malini, D. M. (2013). Pengaruh Ekstrak Etanol dan Spinasterol Daun Senggugu (Clerodendron serratum L.) Terhadap Kualitas Sperma Mencit (Mus musculus L.). Journal of Applied Sciences, 3(3), 49-54.

Martinez, M., Maceraa, S., De A, Pinheiro, P.F.F., Almeida, B., Tirapelli, C., Mar-tins, O., Mello, W., Padovani, D. & Martinez, F. (2019). Structural Evalua-tion of the Effects of Chronic Ethanol Ingestion on the Testes. Tissue and Cell, 41(3), 199-205.

Meriani, H. (2017). Pengaruh Pemberian Vi-tamin E Terhadap Jumlah Sel Sperma Mencit (Mus musculus L.) yang Dipa-par Tuak. Jurnal Keperawatan Imelda, 3(1), 919-200.

Muzdalifa, D. & Jamal, S. (2019). Uji Aktivitas Antioksidan Ekstrak Fraksi Kulit Biji Kopi Robusta (Coffea canephora Pierre ex A. Froehner) terhadap Pereak-si DPPH (1,1- Difenil-2-Prikihildrazil). Indonesia Natural Research Pharmaeu-tical Journal, 6(2), 41-50.

Parhizkar, S., Yusoff, M. J. & Dollah, M. A., (2013). Effect of Phaleria macrocarpa on Sperm Characteristics in Adult Rats. Advanced Pharmaceutical Bulletin, 3(2), 345-352.

Setiawan, H. & Yunianto, I. (2015). Aktivitas Ekstrak Etanol daun Jambu Mete (Anacardium occidentale L.) terhadap Mencit (Mus musculus L.) sebagai Ma-teri Pembelajaran Siswa SMA Kelas XI IPA untuk Mencapai KD 3.12 Kuriku-lum 2013. Jupemasi-PBio, 1(2), 212- 218.

Setiawan, H. & Yunianto, I. (2016). Pe-ngaruh Ekstrak Etanol Daun Jambu Mete (Anacardium occidentale L.) terhadap Perkembangan Sel Spermatogenik Mencit (Mus musculus L.). Prosiding Symbion. 405-416.

Siburian, J. & Marlinza, R. (2009). Efek Pemberian Ekstrak Akar Pasak Bumi (Eurycoma longifolia J.) Pada Tahap Prakopulasi Terhadap Fertilitas Mencit (Mus musculus L.) Betina. Jurnal Bio-spesies, 2(2), 24-30.

Suvarna, S. K., Layton, C. & Bancroft, J. D. (2012). Bancrofts Theory and Prac-tice of Histological Techniques. United Kingdom: Elsevier.

Tablado, L., Pérez-Sánchez, F., Núñez, J., Núñez, M. & Soler, C. (1998). Effects of Exposure to Static Magnetic Fields on the Morphology and Morphometry of Mouse Epididymal Sperm. Bioelec-tromagnetics, 19(6), 377–383.

Udoha, M. U., Okolie, N. J. & Ijioma, S. N. (2019). Chronic Oral Administration of Ocimum gratissimum Leaf Extract Induced Gastrointestinal Necrosis in Treated Albino Rats. European Journal of Medical Plants, 27(3), 1-9.

Utami, N. W. A. (2016). Modul Antropometri. Bali: Fakultas Kedokteran Universitas Udayana.

Vinnata, N. N., Salni. & Nita, S. (2018). Pem-berian Fraksi Daun Kemangi (Ocimum americanum L.) terhadap Spermatozoa Tikus Putih Jantan (Rattus norvegicus). Jurnal Kesehatan, 9(3), 366-375.

Wael, S. & Mahulette, F. (2013). Pengaruh Pemberian Minuman Tradisional Arak Ambon (Sopi) Terhadap Spermatozoa Tikus Sprague Dawley. Bimafika, 4(2), 495-498.

Wyrobek. A. J., & Bruce, W. R. (1975). Chemical Induction of Sperm Abnor-malities in Mice. Cell Biology, 72(11), 4425-4429.

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Published

30-11-2020

How to Cite

Setiawan, H., Maliza, R., Maulana, S. A., & Hisbullah, M. I. (2020). The Effect of Coffee Fruit Skin Extract on Sperm Characteristics And Testicular of Mice With Ethanol-Induced. Jurnal Biodjati, 5(2), 259–270. https://doi.org/10.15575/biodjati.v5i2.9280

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Vol. 5 No. 2 (2020): November

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