Immunomodulatory Activity of Allium sativum, Curcuma mangga, and Acorus calamus Combination Nanoparticle on Mice Leukocytes Profile

Authors

  • Bayyinatul Muchtaromah Biology Magister, State Islamic University of Maulana Malik Ibrahim Malang, Indonesia
  • Nur Izzah Analisa Department of Biology, Faculty of Science and Technology, UIN Maulana Malik Ibrahim, Jl. Gajayana No.50, Malang, East Java, Indonesia, 65144, Indonesia
  • Mujahidin Ahmad Department of Biology, Faculty of Science and Technology, UIN Maulana Malik Ibrahim, Jl. Gajayana No.50, Malang, East Java, Indonesia, 65144, Indonesia
  • Prilya Dewi Fitriasari Department of Biology, Faculty of Science and Technology, UIN Maulana Malik Ibrahim, Jl. Gajayana No.50, Malang, East Java, Indonesia, 65144
  • Soraya Habibi Department of Biology, Faculty of Math and Science, Universitas Terbuka, Jl. Mayjen Sungkono No.9, Bumiayu, Malang, East Java, Indonesia, 65135, Indonesia
  • Alfiah Hayati Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo No.47, Surabaya, East Java, Indonesia, 60132, Indonesia
  • Ely Nuril Fajriyah Biology Magister, State Islamic University of Maulana Malik Ibrahim Malang

DOI:

https://doi.org/10.15575/biodjati.v7i1.11879

Keywords:

Nanoparticle, Allium sativum, Curcuma mangga, Acorus calamus, Leukocyte

Abstract

Immunomodulators are substances acting as stimulants or suppressants against the immune system, either to specific or non-specific activity. The non-specific through the production of leukocytes as the first response in fighting against an attacking antigen. Meanwhile, the specific, hrough recognition of antigens by lymphocytes during reattack. This research aimed to determine the immunomodulatory activity of the extracts combination nanoparticles of Allium sativum, Curcuma mangga, and Acorus calamus on the leukocyte profile of mice. This study used 5 treatments and 6 repetitions. The treatment groups were K- (untreated group), P1 (extracts combination nanoparticle dose of 25 mg/kg), P2 (extracts combination nanoparticle dose of 50 mg / kg), P3 (subur kandungan herbal medicine dose of 75 mg / kg), and P4 (Clomiphene citrate dose of 0.9 mg / kgBW). The parameters used included the total number of leukocytes and their differential value. The data that met the parametric assumptions, such as normally distributed and homogeneous were examined using the One Way ANOVA test, and when there was a significant difference, it was processed with the Duncan assessment. While those that did not meet the assumptions were evaluated using a non-parametric analysis. The statistical results showed that administration of extracts combination nanoparticles of A. sativum, C. mangga, and A. calamus at doses of 25 mg/kg and 50 mg/kg were able to suppress the inflammatory reaction by decreasing the total number of leukocytes. However, the differential leukocyte count was able to maintained or modulate immune system, indicating by the percentage of neutrophil, basophil, and eosinophil in the normal range. At a dose of 50 mg/kg, decreased the percentage of lymphocytes, while for monocytes, all dosage ranges were able to increase their number.

Author Biography

Bayyinatul Muchtaromah, Biology Magister, State Islamic University of Maulana Malik Ibrahim Malang

Biology, A

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Published

30-05-2022

How to Cite

Muchtaromah, B., Analisa, N. I., Ahmad, M., Fitriasari, P. D., Habibi, S., Hayati, A., & Fajriyah, E. N. (2022). Immunomodulatory Activity of Allium sativum, Curcuma mangga, and Acorus calamus Combination Nanoparticle on Mice Leukocytes Profile. Jurnal Biodjati, 7(1), 25–35. https://doi.org/10.15575/biodjati.v7i1.11879

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Vol. 7 No. 1 (2022): May

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