Anti-inflammatory Activity of Bajakah Stem (Spatholobus littoralis Hassk.) Ethanolic Extract in Carrageenan-Induced Paw Edema Mice


Diah Wulandari Rousdy(1*), Elvi Rusmiyanto Pancaning Wardoyo(2), Siti Ifadatin(3)

(1) Department Biology, Faculty of Mathematics and Natural Sciences, University Tanjungpura, Jl. Prof. Dr. H. Hadari Nawawi, Pontianak, West Kalimantan, Indonesia, 7811, Indonesia
(2) Department Biology, Faculty of Mathematics and Natural Sciences, University Tanjungpura, Jl. Prof. Dr. H. Hadari Nawawi, Pontianak, West Kalimantan, Indonesia, 7811, Indonesia
(3) Department Biology, Faculty of Mathematics and Natural Sciences, University Tanjungpura, Jl. Prof. Dr. H. Hadari Nawawi, Pontianak, West Kalimantan, Indonesia, 7811,  
(*) Corresponding Author

Abstract


Bajakah tampala plant (Spatholobus littoralis Hassk.) has been used for traditional medicine by the Kalimantan Dayak tribe in the treatment of cancer. This study aimed to find out the potential of ethanol extract of the stem of Bajakah tampala (Spatholobus littoralis Hassk.) as an anti-inflammatory agent in carrageenan-induced mice. The method used was a Completely Randomized Design with five treatments and five replications. The treatments consisted of a water-treated mouse as a negative control, sodium diclofenac 30,8 mg kg-1 as a positive control, and an extract of bajakah dose 2.5; 250; 1250 mg kg-1 BW. The dose of bajakah extract was given orally one hour before carrageenan induction. The inflammation induction used the Winter method by injecting 0.15 ml of 2% carrageenan suspension into the soles of mice. The diameter of edema was measured for 210 minutes. The results showed that the diameter of the mice soles showed a significant increase in all groups after the carrageenan injection. The value of AUC (Area Under the Curve) of bajakah extract dose (2.5; 250; 1250 mg kg-1) from the 150th minute to the 210th minute did not show significant differences from one another. The sodium diclofenac as positive control gave the lowest total AUC value of 236 mm.minute, followed by bajakah extract at a dose of 2.5 mg kg-1 BW of 239.22 mm.minute. The calculation results of the percentage of the inflammation inhibition showed that the administration of ethanol extract of the stem of Bajakah, Spatholobus littoralis, at the lowest dose of 2.5 mg kg-1 BW provide the best inflammation inhibition with 19.21% inhibitory value, nearly approaching the positive control of diclofenac sodium with an inhibitory value of 21.53%.


Keywords


edema, extract, inflammation, Spatholobus littoralis

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References


Abraham, I., Joshi, R., Pardasani, P. & Pardasani, R. T. (2011). Recent Advances in 1,4-benzoquinone Chemistry. Journal of the Brazillian Chemical Society, 22(3), 385-421

Altman, R., Bosch, B., Brune, K., Patrignani, P. & Young, C. (2015). Advances in NSAID Development: Evolution of Diclofenac Products Using Pharmaceutical Technology. Drugs, 75, 859-877.

Apridamayanti, P., Sanera, F. & Robiyanto, R. (2018). Antiinflammatory Activity of Ethanolic Extract from Karas Leaves (Aquilaria malaccencis Lamk.). Pharmaceutical Sciences and Research, 5(3), 152 – 158.

Banjarnahor, S. D. S. & Artanti, N. (2014). Antioxidant Properties of Flavonoids. Medical Journal of Indonesia, 23(4), 239-244.

Brunetti, C., Ferdinando, M. D., Fini, A., Pollastri, S. & Tattini, M. (2013). Flavonoids as Antioxidants and Development Regulators: Relative Significance in Plants and Humans. Int. J. Mol. Sci, 14(2), 3540-3555.

Chen, D.H., Luo, X., Yu, M.Y., Zhao, Y.Q. & Cheng, Y.F. (2004). Effect of Spatholobus suberectus on The Bone Marrow Cells and Related Cytokines of Mice. Zhongguo Zhong Yao Za Zhi, 29, 352–355.

Choi, F. J. & Kim, G. H. (2014). The Antioxidant Activity of Daidzein Metabolites, Oldesmethylangolensin and Equol, in HepG2 cells. Molecular Medicine Report, 9(1), 328-332.

de Groot, C. & Muller-Goymann, C. C. (2016). Saponin Interactions with Model Membrane System-Langmuir Monoloayer Studies, Hemolysis and Formation of ISCOMs Planta Med, 82, 1496-1512

Huang, Y., Chen, L., Feng., L. & Guo, F. (2013). Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity. Molecules, 18, 7549-7556.

Inami, K., Asada, Y., Harada, T., Okayama, Y., Usui, N. & Mochizuki, M. (2019). Antimutagenic Components in Spatholobus suberectus Dunn Against N-methyl-Nnitrosourea. Genes and Environment Journal, 41, 22-29.

Kaur, G., Hamid, H., Ali, A., Alam, M. S. & Athar, M. (2004). Antiinflammatory Evaluation of Alcoholic Extract of Galls of Quercus infectoria. J. Ethnopharmacology, 90(2-3), 285-292

Li, R. W., David Lin, G., Myers, S. P. & Leach, D. N. (2003). Anti-inflammatory Activity of Chinese Medicinal Vine Plants. Journal of Ethnopharmacology, 85, 61–67.

Liu, X. Y., Zhang, Y. B., Yang, X. W., Yang, Y. F., Xu, W., Zhao, W., Peng, K. F., Gong, Y., Liu, N.F. & Zhang, P. (2019). Anti-Inflammatory Activity of Some Characteristic Constituents from the Vine Stems of Spatholobus suberectus. Molecules, 24, 3750.

Myers, M. J., Deaver, C. M. & Lewandowski, A.J. (2019). Molecular Mechanism of Action Responsible for Carrageenan-Induced Inflammatory Response. Molecular Immunology, 109, 38-42.

Necas, J. & Bartosikova, L. (2013). Carrageenan: A Review. Veterinarni Medicina, 58, 187-205.

Ninkaew S. & Chantaranothai. (2014). The Genus Spatholobus Hassk. (Leguminosae-Papilionoideae) in Thailand, Tropical Natural History, 14(2), 87-99.

Pacheco-Quito E., Ruiz-Carlo, R. & Veiga, M. D. (2020). Carrageenan: Drug Delivery Systems and Other Biomedical Applications. Marine Drugs, 18(583), 1-39.

Peng, F., Zhu H., Meng, C., Ren, Y., Dai O. & Xiong L. (2019). New Isoflavanes from Spatholobus suberectus and Their Cytotoxicity against Human Breast Cancer Cell Lines. Molecules, 24(18), 3218.

Ricciotti, E. & Fitzgerald, G.A. (2011). Prostaglandins and Inflammation. Arterioscler Thromb Vasc Biol, 31(5), 986–1000.

Sahlan, M., Devina, A., Pratami, D. K., Situmorang, H., Farida, S., Munim,A., Kusumoputro, B., Yohda, M., Faried A., Gozan M. & Ledyawati, M. (2019). Anti-inflammatory Activity of Tetragronula Species from Indonesia. Saudi Journal of Biological Sciences, 26(7), 1531-1538.

Salvo, F., Fourrier-Reglat, A., Bazin, F., Robinson, P., Riera-Guardia, N. & Haag, M. (2011). Cardiovascular and Gastrointestinal Safety of NSAIDs: A Systematic Review of Meta-analyses of Randomized Clinical Trials. Clin Pharmacol Ther, 89(6), 855–66.

Saputera, M. M. A., Marpaung, T. W. A. & Ayuchecaria, N. (2019). Konsentrasi Hambat Minimum (KHM) Kadar Ekstrak Etanol Batang Bajakah Tampala (Spatholobus littoralis Hassk) Terhadap Bakteri Escherichia coli Melalui Metode Sumuran. Jurnal Ilmiah Manuntung, 5(2), 167-173.

Sun, J., Zhang, G., Zhang, Y., Nan, N., Sun, X., Yu, M., Wang, H., Li, J. & Wang, X. (2016). Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Supressing ER MAPK PI3K/AKT Pathway. Evidenced Based Complementary and Alternative Medicine, 1-13.

Yusuf, S.M., Rochmawaty, E. & Nusjirwan, R. (2014). Bixa Orellana L Leaf Infusion as an Anti-inflammatory Agent in Carrageenan-induced Wistar Rat. Althea Medical Journal, 1(2), 60-64.

Wardah & Sundari, S. (2019). Ethnobotany Study of Dayak Society Medicinal Plants Utilization in Uut Murung District, Murung Raya Regency, Central Kalimantan. IOP Conf. Series: Earth Environ. Sci. 298 012005.

Wardoyo, E.R.P., Nugroho, L. H., Santosa & Moeljopawiro, S. (2011). Efek Sitotoksik Ekstrak Kloroform, Methanol dan Air Buah Brucea javanica (L.) Merr. Terhadap Sel Kanker Payudara (T47D). Berkala Penelitian Hayati, 4D, 13–16.

Winter, C. A., Risley, E. A. & Nuss, G. W. (2002). Carrageenanin-induced Udem in Hind Paw of the Rat as an Assay for Antiinflamatory Drugs. Proceding Soc. Exp. Sol. Med. 111, 544-547.




DOI: https://doi.org/10.15575/biodjati.v7i1.14126

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