Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.)


Untung Susilo(1*), Farida Nur Rachmawati(2), Eko Setio Wibowo(3)

(1) Biology Faculty, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 63, Purwokerto, Central Java, Indonesia, 53122
(2) Biology Faculty, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 63, Purwokerto, Central Java, Indonesia, 53122
(3) Biology Faculty, Universitas Jenderal Soedirman, Jl. Dr. Soeparno No. 63, Purwokerto, Central Java, Indonesia, 53122
(*) Corresponding Author

Abstract


Studies on morphology, growth, and reproduction have been carried out on wild Javaen barb, but there was no information on its digestive capacity; therefore, the research was conducted to determine protease and amylase activities in the digestive tract. This study used a total of 50 barbs with body weights between 13.56 -128.93g / fish. The measurement of enzyme activity was carried out using the spectrophotometer method. The results showed that differences in fish size resulted in differences in protease activity, but not for amylase. Fish with a small size have a higher protease activity than fish with a larger size. The protease activity did not differ between pH 6.9 to 10.0 but was higher than pH 12.5. Protease activity also did not vary between the anterior and posterior intestine and between 30-50°C. Amylase activity also found no difference between the anterior and posterior intestine, but there was a difference in activity between temperatures of 30-50°C. In conclusion, protease activity occurs in a neutral to alkaline environment, and there were differences in protease activity between different body sizes but not between intestinal segments. Amylase activity occurs throughout the intestine and decreases at temperatures of 50°C.


Keywords


digestive enzyme, gut segment, Javaen barb, pH, temperature Hassk

Full Text:

PDF

References


Abidin, D. A. Z., Hashim, M., Das, S. K., Rahim, S. M. & Mazlan, A. G. (2016). Enzymatic Digestion of Stomachless Fish Zenarchopterus buffonis. Aquaculture, Aquarium, Conservation & Legislation-International Journal of the Bioflux Society (AACL Bioflux), 9(3), 695-702.

Aissaoui, N., Marzouki, M. N. & Abidi, F. (2017). Purification and Biochemical Characterization of a Novel Intestinal Protease from Scorpaena notata. International Journal of Food Properties, 20(sup2), 2151–2165.

Candiotto, F. B., Freitas-Júnior, A. C. V, Neri, R. C. A., Bezerra, R. S., Rodrigues, R. V, Sampaio, L. A. & Tesser, M. B. (2017). Characterization of Digestive Enzymes from Captive Brazilian Flounder Paralichthys orbignyanus. Brazilian Journal of Biology, 78, 281–288.

Caruso, G., Denaro, M. G. & Genovese, L. (2009). Digestive Enzymes in Some Teleost Species of Interest for Mediterranean Aquaculture. The Open Fish Science Journal, 2, 74–86.

Champasri, C. & Champasri, T. (2017). Biochemical Characterization, Activity Comparison And Isoenzyme Analysis of Amylase and Alkaline Proteases in Seven Cyprinid Fishes. J Fish Aquat Sci, 12, 264–272.

Concha-Frias, B., Alvarez-González, C. A., Gaxiola-Cortes, M. G., Silva-Arancibia, A. E., Toledo-Agüero, P. H., Martínez-García, R. & Arias-Moscoso, J. L. (2016). Partial Characterization of Digestive Proteases in The Common Snook Centropomus undecimalis. International Journal of Biology, 8(4), 1–11.

Drewe, K. E., Horn, M. H., Dickson, K. A. & Gawlicka, A. (2004). Insectivore to Frugivore: Ontogenetic Changes in Gut Morphology and Digestive Enzyme Activity in The Characid Fish Brycon guatemalensis from Costa Rican rain forest streams. Journal of Fish Biology, 64(4), 890–902.

Furne, M., Hidalgo, M. C., Lopez, A., Garcia-Gallego, M., Morales, A. E., Domezain, A. & Sanz, A. (2005). Digestive Enzyme Activities in Adriatic Sturgeon Acipenser naccarii and Rainbow Trout Oncorhynchus mykiss. A comparative study. Aquaculture, 250(1–2), 391–398.

Gangadhar, B., Sridhar, N., Umalatha, H., Ganesh, H., Simon, A. R. T. & Jayasankar, P. (2017). Digestibility and Digestive Enzyme Activity in Labeo Fimbriatus (Bloch, 1795) Fed Periphyton Grown on Sugarcane Bagasse. Indian Journal of Fisheries, 64(1), 37–43.

Gioda, C. R., Pretto, A., Freitas, C. de S., Leitemperger, J., Loro, V. L., Lazzari, R. & Salbego, J. (2017). Different Feeding Habits Influence The Activity of Digestive Enzymes in Freshwater Fish. Ciência Rural, 47(3), 1-6.

Hlophe, S. N., Moyo, N. A. G. & Ncube, I. (2014). Postprandial Changes in pH and Enzyme Activity From The Stomach and Intestines of Tilapia rendalli (Boulenger, 1897), Oreochromis mossambicus (Peters, 1852) and Clarias gariepinus (B urchell, 1822). Journal of Applied Ichthyology, 30(1), 35–41.

Izvekova, G. I., Solovyev, M. M., Kashinskaya, E. N. & Izvekov, E. I. (2013). Variations in the Activity of Digestive Enzymes Along The Intestine of The Burbot Lota lota expressed by Different Methods. Fish Physiology and Biochemistry, 39(5), 1181–1193.

Jahantigh, M. (2015). Characteristics of Some Digestive Enzymes in Sobaity, Sparidentex hasta. Iranian Journal of Veterinary Medicine, 9(3), 213–218.

Klahan, R., Areechon, N., Yoonpundh, R. & Engkagul, A. (2009). Characterization and Activity Of Digestive Enzymes in Different Sizes of Nile Tilapia (Oreochromis niloticus L.). Kasetsart Journal - Natural Science, 43(1), 143–153.

Kottelat, M., Whitten, A. J., Kartikasari, S. N. & Wirjoatmodjo, S. (1993). Freshwater Fishes of Western Indonesia and Sulawesi. Jakarta: Periplus Edition Limited.

Krogdahl, Å., Sundby, A., & Holm, H. (2015). Characteristics of Digestive Processes in Atlantic Salmon (Salmo Salar). Enzyme pH Optima, Chyme pH, and Enzyme Activities. Aquaculture, 449, 27–36.

Kuz’mina, V. V., Skvortsova, E. G., Zolotareva, G. V. & Sheptitskiy, V. A. (2011). Influence of pH Upon `The Activity of Glycosidases and Proteinases of Intestinal Mucosa, Chyme and Microbiota in Fish. Fish physiology and biochemistry, 37(3), 345-353.

Martínez-Cárdenas, L., Álvarez-González, C. A., Hernández-Almeida, O. U., Frías-Quintana, C. A., Ponce-Palafox, J. T. & Castillo-Vargasmachuca, S. (2017). Partial Characterization of Digestive Proteases in The Green Cichlid, Cichlasoma beani. Fishes, 2(4), 1-11

Martínez-Cárdenas, L., Frías-Quintana, C. A., Álvarez-González, C. A., Jiménez-Martínez, L. D., Martínez-García, R., Hernández-Almeida, O. U. & Ponce-Palafox, J. T. (2020). Partial Characterization of Digestive Proteases in Juveniles of Microphis brachyurus (short-tailed pipefish)(Syngnathiformes: Syngnathidae). Neotropical Ichthyology, 18(2): 1-15

Murashita, K., Furuita, H., Matsunari, H., Yamamoto, T., Awaji, M., Nomura, K. & Tanaka, H. (2013). Partial Characterization and Ontogenetic Development of Pancreatic Digestive Enzymes In Japanese Eel Anguilla japonica larvae. Fish Physiology and Biochemistry, 39(4), 895–905.

Peña, E., Hernández, C., Álvarez-González, C. A., Ibarra-Castro, L., Puello-Cruz, A. & Hardy, R. W. (2015). Comparative Characterization of Protease Activity in Cultured Spotted Rose Snapper Juveniles (Lutjanus guttatus) | Caracterización Comparativa de La Actividad de La Proteasa en Juveniles Cultivados De Pargo Flamenco (Lutjanus guttatus). Latin American Journal of Aquatic Research, 43(4), 641–650.

Pujante, I. M., Díaz‐López, M., Mancera, J. M. & Moyano, F. J. (2017). Characterization of Digestive Enzymes Protease and Alpha‐Amylase Activities in The Thick‐Lipped Grey Mullet (Chelon labrosus, Risso 1827). Aquaculture Research, 48(2), 367–376.

Rahmah, S., & El-sayed, R. H. A. M. (2020). Digestive Proteases and In Vitro Protein Digestibility in Bagrid Catfish Mystus nemurus ( Cuvier and Valenciennes 1840 ), Aquaculture Research, 51(11), 4613–4622

Sankar, H., Jose, J., Varadarajan, R., Bhanu, S. V, Joy, S. & Philip, B. (2014). Functional Zonation of Different Digestive Enzymes in Etroplus suratensis and Oreochromis mossambicus. International Journal of Scientific and Research Publications, 4(5), 1–5.

Sila, A., Haddar, A., Sayari, N., Nasri, M., & Bougatef, A. (2015). Digestive alkaline proteases from the Tunisian barbell (Barbus callensis): Characterization and application as A Detergent Additive, in Chicken Feather-Degradation and as A Dehairing Agent. Iranian Journal of Fisheries Sciences, 14(3), 672–686.

Suryaningsih, S., Sagi, M., Nitimulyo, K. H. & Hadisusanto, S. (2012). Beberapa Aspek Pemijahan Ikan Brek Puntius orphoides (Valenciennes, 1842) di Sungai Klawing Purbalingga, Jawa Tengah [Spawning Aspects Of Javaenen Barb Puntius Orphoides (Valenciennes, 1842) in Klawing River, Purbalingga, Central Javaen]. Jurnal Iktiologi Indonesia, 12(1), 35–48.

Susatyo, P., Sugiharto, S., Hana, H., & Chasanah, T. (2016). Effects of Some Feed Supplements Types to The Growth of Javaenen Barb/Brek Fish (Puntius orphoides) Second Filial As Domestication Product. Biosaintifika: Journal of Biology & Biology Education, 8(3), 278–285.

Susilo, U., E. Yuwono dan F.N. Rachmawati, 2012. Aktivitas Protease dan Amilase Digesti Benih Ikan Gurami, Osphronemus gouramy Lac. Makalah Seminar Nasional Indo Aqua-Fita. 8-11 Juni 2012, Makasar, Indonesia.

Susilo, U., Sukardi, P., & Affandi, R. (2018). The Age-Dependent Activities of Digestive Enzymes in Rasbora, Rasbora lateristriata Blkr.,(Pisces: Cyprinidae). Molekul, 13(1), 80–91.

Thongprajukaew, K., & Kovitvadhi, U. (2013). Effects of Sex On Characteristics and Expression Levels of Digestive Enzymes In The Adult Guppy Poecilia reticulata. Zoological Studies, 52(1), 1–8.

Thongprajukaew, K., Kovitvadhi, U., Engkagul, A., & Torrissen, K. R. (2010). Temperature and pH Characteristics of Amylase and Lipase at Different Developmental Stages of Siamese Fighting Fish (Betta splendens Regan, 1910). Kasetsart J.(Nat. Sci.), 44, 210–219.

Tian, H., Meng, Y., Li, C., Zhang, L., Xu, G., Shi, Y. & Ma, R. (2019). A study of The Digestive Enzyme Activities in Scaleless Carp (Gymnocypris przewalskii) on The Qinghai-Tibetan Plateau. Aquaculture Reports, 13, 100174.

Tongsiri, S., Mang-Amphan, K. & Peerapornpisal, Y. (2010). Characterization of Amylase, Cellulase And Proteinase Enzyme in Stomach and Intestine of The Mekong Giant Catfish Fed With Various Diets Consisting of Spirulina. Current Research Journal of Biological Sciences, 2(4), 268–274.

Umalatha, S. N., Kushwaha, J. P. & Gangadhar, B. (2016). Digestive Enzyme Activities In Different Size Groups and Segments of The Digestive Tract in Labeo rohita (Day, 1878). J Aquac Mar Biol, 4(5), 1-6

Xiong, D. M., Xie, C. X., Zhang, H. J. & Liu, H. P. (2011). Digestive Enzymes Along Digestive Tract of a Carnivorous Fish Glyptosternum maculatum (Sisoridae, Siluriformes). Journal of Animal Physiology and Animal Nutrition, 95(1), 56–64.

Ye, J. S., Chen, X. J. & Zhu, Y. Y. (2013). Influence of pH on survival, Growth and Activities of Digestive Enzymes of Odontobutis obscures. Advance Journal of Food Science and Technology, 5(9), 1234–1237.and activities of digestive enzymes of Odontobutis obscures. Advance Journal of Food Science and Technology, 5(9), 1234–1237.




DOI: https://doi.org/10.15575/biodjati.v7i1.15535

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Jurnal Biodjati

License URL: https://creativecommons.org/licenses/by-nc-nd/4.0/

Indexing By :

      

      

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

View My Stats