Allopurinol Induction on Histopathological Structure of the Liver in Male Mice (Mus musculus)


Dian Fita Lestari(1*), Fatimatuzzahra Fatimatuzzahra(2), Agnes Petra Sianipar(3), Shahnaz Shabrina Wulansari(4)

(1) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jl. W.R. Supratman, Bengkulu, Indonesia 38371, Indonesia
(2) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jl. W.R. Supratman, Bengkulu, Indonesia 38371, Indonesia
(3) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jl. W.R. Supratman, Bengkulu, Indonesia 38371, Indonesia
(4) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jl. W.R. Supratman, Bengkulu, Indonesia 38371, Indonesia
(*) Corresponding Author

Abstract


Allopurinol is used to reduce total uric acid levels in the body into oxypurinol which can inhibit xanthine oxidase. Allopurinol inhibits the precursors of uric acid formation, xanthine, and hypoxanthine. However, consumption of the drugs can cause side effects on the liver. The aim of the research was to determine the effect of allopurinol induction on the liver histopathology of male mice (Mus musculus) DDY strain. The method used in this research was an experimental design used post-test only that was divided into 4 groups of 4 mice per group. The control group (P0) was given 0.5% Na-CMC, and groups I, II, and III (P1, P2, and P3) were induced by allopurinol at 10 mg/kg BW, 20 mg/kg BW, and 30 mg/kg BW for 14 days. Allopurinol induction was performed by oral gavage. The results of the research showed that treatment with allopurinol caused changes in the mice’s body weight, liver index, liver morphology, and histological structure of the liver tissue, including necrosis, steatosis, leukocyte infiltration, binuclear hepatocytes, hepatocyte swelling, congestion, sinusoid dilatation, and hemorrhage. The level of liver damage increased in line with the dose used. This research indicated that the higher the allopurinol level, the higher the level of alteration in the liver section structure. Long-term use of allopurinol can cause damage to the structure of mice liver (liver toxicity). 


Keywords


allopurinol, histopathology, liver

Full Text:

PDF

References


Albir A. A. & Al-Kaisy A.Z. (2019). Histological Investigation of the Hepatic Tissue in Mice Induced by Soft Laser with Different Doses. Biomedical & Pharmacology Journal, 12(2), 989-992. DOI: 10.13005/bpj/1726.

Cendrianti F., Muslichah, S. & Umayah E. (2014). Uji Aktivitas Antihiperurisemia Ekstrak n-Heksana, Etil Asetat, dan Etanol 70% Daun Tempuyung (Sonchus arvensis L.) pada Mencit Jantan Hiperurisemia. Jurnal Pustaka Kesehatan, 2(2), 205-209. Retrived from: https://jurnal.unej.ac.id/index.php/JPK/article/view/1083.

Cengiz S., Cavaz, L., Yurdakoc, C. & Aksu, S. (2012). Inhibition of Xanthine Oxidase by Caulerpenyne from Caulerpa prolifera. Turkish Journal of Biochemistry, 37(4), 445-451. DOI: 10.5505/TJB.2012.98698.

Ceriana, R., Fitri, S. & Sari, W. (2018). Perubahan Berat Organ Hati, Ginjal, Limfa, Otak, Lambung, Testis, Jantung dan Paru-paru Mencit yang Diberi Ekstrak Batang Sipatah-patah (Cissus quadrangula Salisb.). Journal of Healthcare Technology and Medicine, 4(1), 127-134. DOI: 10.33143/jhtm.v4i1.201.

Ekinci, O., Eren, T., Gapbarov, A., Gacemer, M., Ozkinet, T. & Celik, F. (2019). Effects of Octreotide and Allopurinol on Liver Histology and Functions in Surgical Jaundice: An Experimental Study. Albanian Journal of Trauma and Emergency Surgery, 3 (1), 1-10. DOI: 10.32391/ajtes.v3i1.34.

Fitri, R. (2017). Pengaruh Terapi Ekstrak Teh Hijau (Camellia Sinensis) Terhadap Kadar Asam Urat, Xantin Oksidase (XOD), Malondialdehid (MDA) Dan Gambaran Histopatologi Hepar pada Tikus (Rattus Novergicus) Hiperurisemia. Thesis. Malang: Universitas Brawijaya. Retrived from: http://repository.ub.ac.id/id/eprint/9289/.

Fortes, R.C. (2017). Nutritional Implications in Chronic Liver Diseases. Journal of Liver Research, Disorders & Therapy, 3(5), 131-135. DOI: 10.15406/jlrdt.2017.03.00071.

Guzik P., Kim, C., Kumbum, K. (2013). Severe Allopurinol-Induced Liver Injury. The American Journal of Gastroenterology, 119, 1311-1312. DOI: 10.14309/01.ajg.0000598956.00340.

Hammer, S. J. & McPhee, S. J. (2014). Pathophysiology of Disease: An Introduction to Clinical Medicine. USA: McGraw-Hill.

Hayati, S. & Sunaryo. (2014). Efek Hepatoprotektor Fraksi Asetat Daun Sangitan (Sambucus Canadensis L.) pada Tikus Sprague Dawley. Media Farm, 11(1), 55–61. DOI: 10.12928/mf.v11i1.1397.

Ibrahim, K. E., Al-Mutary, M. G., Bakhiet, A. O. & Khan, H. A. (2018). Histopathology of the Liver, Kidney, and Spleen of Mice Exposed to Gold Nanoparticles. Molecules, 23(8), 1848. DOI: 10.3390/molecules23081848.

Iqbal, U., Siddiqui, H.U., Anwar, H., Chaudhary, A., & Quadri, A.A. (2017). Allopurinol-Induced Granulomatous Hepatitis: A Case Report and Review of Literature. Journal of Investigative Medicine High Impact Case Reports, 1-3. DOI: 10.1177/2324709617728302.

Jayanegara, D. (2017). Gambaran Histopatologi Hati Mencit (Mus musculus) yang Diberi Paparan Ibuprofen Dosis Bertingkat. Skripsi. Makassar: Fakultas Kedokteran Universitas Hasanuddin.

Lailatul, N., Lyrawati, D. & Handaru, M., (2015). Efek Pemberian Asam Alfa Lipoat terhadap Kadar MDA dan Gambaran Histologi pada Hati Tikus Wistar Jantan dengan Diabetes Melitus Tipe 1. Jurnal Kedokteran Brawijaya, 28(3), 170-176. DOI: 10.21776/ub.jkb.2015.028.03.2.

Liu, X., Chen, R., Shang, Y., Jiao, B. & Huang, C. (2008). Lithospermic Acid as a Novel Xanthine Oxidase Inhibitor has Anti-inflammatory and Hypouricemic Effects in Rats. Chem-Biol Interact, 176(3), 137-142. DOI: 10.1016/j.cbi.2008.07.003.

Liu Y., Jiang Y., Dong X.D., et al. (2013). Liver Injury due to Allopurinol: two case reports. Adverse Drug Reaction Journal, 15(4), 231-233. DOI: 10.3760/cma.j.issn.1008-5734.2013.04.020.

Magfirah & Christin, V. (2020). Analisis Profil Bobot Badan Tikus dan Gejala Toksis Pada Pemberian Ekstrak Etanol Daun Parang Romang (Boehmeria virgata) terhadap Tikus Putih (Rattus novergicus). Galenika Journal of Pharmacy, 6(1), 1-6. DOI: 10.22487/j24428744.2020.v6.i1.13928.

Makiyah, A. & Khumaisah, L. L. (2018). Studi Gambaran Histopatologi Hepar Tikus Putih Strain Wistar yang Diinduksi Aspirin Pasca Pemberian Ekstrak Etanol Umbi Iles-iles (Amorphophallus variabilis Bl) Selama 7 Hari. Majalah Kedokteran, 50(2), 93-101. DOI: 10.15395/mkb.v50n2.1323.

Mardiati, S. M. & Sitasiwi, A. J. (2016). Pertambahan Berat Badan Mencit (Mus musculus L.) Setelah Perlakuan Ekstrak Air Biji Pepaya (Carica papaya Linn.) Secara Oral Selama 21 Hari. Buletin Anatomi dan Fisiologi, 1(1), 75-80. DOI: 10.14710/baf.1.1.2016.75-80.

Maulida, A. (2013). Pengaruh Pemberian Vitamin C dan E Terhadap Gambaran Histologi Hepar Mencit (Mus musculus L) yang Dipajankan Monosodium Glutamat (MSG). Skripsi. Medan: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sumatera Utara.

Mulyono, A., Farida, D. H. & Soesanti, N. (2013). Histopatologi Hepar Tikus Rumah (Rattus tanezumi) Infektif Patogenik Leptospira spp. Jurnal Vektora, 5(1), 7-11.

Sarin, S. K., Kumar, M., Eslam, M., George, J., Mahtab, M. et. al. (2020). Liver diseases in the Asia-Pacific region: a Lancet Gastroenterology & Hepatology Commission, Lancet Gastroenterol Hepatology, 5(2), 167-228. DOI: 10.1016/S2468-1253(19)30342-5.

Sijid, S. A., Muthiadin, C., Zulkarnain, Hidayat, A. S. & Amelia, R. R. (2020). Pengaruh Pemberian Tuak Terhadap Gambaran Histopatologi Hati Mencit (Mus musculus) ICR jantan. Jurnal Pendidikan Matematika dan IPA, 11(2), 193-205. DOI: 10.26418/jpmipa.v11i2.36623.

Surasa, N. J., Utami, N. R. & Isnaeni. (2014). Struktur Mikroanatomi Hati dan Kadar Kolesterol Total Plasma Darah Tikus Putih Strain Wistar Pasca Suplementasi Minyak Lemuru dan Minyak Sawit. Biosaintifika, 6(2), 141– 151. DOI: 10.15294/biosaintifika.v6i2.3778.

Westbrook R. H., Dusheiko G. & Williamson. (2016). Pregnancy and Liver Disease. Journal of Hepatology, 64, 933-945. DOI: 10.1016/j.jhep.2015.11.030.

Yulian, M. (2014). Potensi Biodiversitas Indonesia Sebagai Inhibitor Xantina Oksidase dan Antigout. Lantanida Journal, 1(1), 1-15. DOI: 10.22373/lj.v2i1.666.




DOI: https://doi.org/10.15575/biodjati.v7i2.18616

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Jurnal Biodjati

License URL: https://creativecommons.org/licenses/by-nc-nd/4.0/

Indexing By :

      

      

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

View My Stats