An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate

Authors

  • Kahar Muzakhar Biology Department, Faculty of Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, Indonesia, 68121 https://orcid.org/0000-0002-4247-9430
  • Farah Salma Elida Biology Department, Faculty of Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, Indonesia, 68121
  • Ramdhan Putrasetya Biology Department, Faculty of Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, Indonesia, 68121
  • Siswoyo Siswoyo Chemistry Department, Faculty of Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, 68121
  • Rudju Winarsa Biology Department, Faculty of Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, Indonesia, 68121
  • Hidayat Teguh Wiyono Biology Department, Faculty of Mathematic and Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, Indonesia, 68121

DOI:

https://doi.org/10.15575/biodjati.v7i2.20279

Keywords:

coffee pulp, ISH16 bacteria isolate, pectinase, purification, solid state fermentation

Abstract

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.

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2022-11-30

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Vol. 7 No. 2 (2022): November

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