Analysis of Bacterial Diversity In Temple Bricks Using Phenetic Numeric Taxonomy Method

Authors

  • Luluk Alifia Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Campus ITS, Sukolilo, Surabaya, Indonesia, 60111., Indonesia https://orcid.org/0009-0001-8990-0093
  • Enny Zulaika Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Campus ITS, Sukolilo, Surabaya, Indonesia, 60111., Indonesia
  • Soeprijanto Soeprijanto Department of Industrial Chemical Engineering, Faculty of Vocational Studies, Institut Teknologi Sepuluh Nopember, Campus ITS, Sukolilo, Surabaya, Indonesia, 60111., Indonesia

DOI:

https://doi.org/10.15575/biodjati.v9i2.33476

Keywords:

diversity, numerical taxonomy, phenotype, temple Building

Abstract

Temple bricks-based constructions are known to often face various challenges, including physical, chemical, and biological weathering. Previous studies identified various biological factors contributing to brick weathering including exudate produced by microorganisms, such as bacteria. In addition, bacteria often live synergistically and antagonistically with other species, exhibiting diverse morphological and physiological traits (bacterial diversity). Various methods have been developed to explore bacterial diversity, with phenetic numerical taxonomy being the most popular. Therefore, this study aims to determine bacterial diversity on the surfaces of temple bricks using phenetic numerical taxonomy method. Bacterial isolation was carried out aseptically, followed by labeling and transferring the isolate to the laboratory for further tests. The tests carried out included morphological characterization, biochemical assays, physiological reactions, and potential enzymatic activities. Subsequently, dendrogram was constructed using MVSP (Multi-Variate Statistical Package) software with isolated grouping based on the Unweighted Pair Group Method Averages (UPGMA) algorithm. The similarity between isolates was analyzed using the Simple Matching Coefficient (SSM) similarity value. The dendrogram analysis revealed the presence of 3 clusters namely A (4 isolates), B (1 isolate), and C (2 isolates), with a similarity index of 0,543 to 0,857. Clusters A, B, and C had a similarity index of ≤0.700, indicating the occurrence of distinct species in each cluster. Based on the profile-matching results of key characters, the 7 bacterial isolates were identified as belonging to the genera Bacillus, Corynebacterium, and Mycobacterium.

References

Afrelia, K., Sugireng, & Tasnim. (2021). Dentifikasi Keragaman Genetik Isolat Bakteri Mekonium Bayi Baru Lahir Dengan Metode PCR-RAPD (Random Amplified Polymorphic DNA). Jurnal MediLab Mandala Waluya, 6(1), 43–48. https://doi.org/https://doi.org/10.36566/medilab.v5i1%20juli.148

Akihary, C. V., & Kolondam, B. J. (2020). Pemanfaatan gen 16S rRNA Sebagai Perangkat Identifikasi Bakteri Untuk Penelitian-Penelitian Di Indonesia. Pharmacon, 9(1), 16. https://doi.org/10.35799/pha.9.2020.27405

Efiyanti, L., & Hidayat, A. (2017). Seleksi Jamur Pelapuk Putih Hutan Tropis Indonesia Sebagai Penghasil Enzim Lakase (Lac) dan Mangan Peroksidase (MnP). Jurnal Penelitian Hasil Hutan, 35(3), 185–195. https://doi.org/10.20886/jphh.2017.35.3.185-195

Fatmawati, I. (2018). Evaluasi Dampak Penggunaan Bahan Kimia Selama Kegiatan Pemugaran Candi Brahu. Jurnal Konservasi Cagar Budaya Borobudur, 12(1), 37–47. https://doi.org/10.33374/jurnalkonservasicagarbudaya.v12i1.183

Hanson, A. (2016). Oxidative-Fermentative Test Protocol. September 2008, 1–7.

Holt, J. G., & Smith, R. S. B. E. G. . M. N. R. (1994). Bergey’s Manual of Determinative Bacteriology.

Kigawa, R., Sano, C., Nishijima, M., Tazato, N., Kiyuna, T., Hayakawa, N., Kawanobe, W., Udagawa, S., Tateishi, T., & Sugiyama, J. (2013). Investigation Of Acetic Acid Bacteria Isolated From The Kitora Tumulus In Japan And Their Involvement in The Deterioration of The Plaster of The Mural Paintings. Studies in Conservation, 58(1), 30–40. https://doi.org/10.1179/2047058412Y.0000000040

Lepinay, C., Mihajlovski, A., Touron, S., Seyer, D., Bousta, F., & Di Martino, P. (2018). Bacterial Diversity Associated With Saline Efflorescences Damaging The Walls Of A French Decorated Prehistoric Cave Registered as a World Cultural Heritage Site. International Biodeterioration and Biodegradation, 130(March), 55–64. https://doi.org/10.1016/j.ibiod.2018.03.016

Meng, H., Zhang, X., Katayama, Y., Ge, Q., & Gu, J. D. (2020). Microbial Diversity And Composition Of The Preah Vihear Temple In Cambodia By High-Throughput Sequencing Based on Genomic DNA and RNA. International Biodeterioration and Biodegradation, 149(January), 104936. https://doi.org/10.1016/j.ibiod.2020.104936

Mihajlovski, A., Gabarre, A., Seyer, D., Bousta, F., & Di Martino, P. (2017). Bacterial Diversity on Rock Surface of The Ruined Part of a French Historic Monument: The Chaalis Abbey. International Biodeterioration and Biodegradation, 120, 161–169. https://doi.org/10.1016/j.ibiod.2017.02.019

Mulyawati, A. I., Ardyati, T., & Jatmiko, Y. D. (2019). Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mare’s Milk Sumbawa. Jurnal Biodjati, 4(1), 21–30. https://doi.org/10.15575/biodjati.v4i1.4194

Munawati, R., Octavia, B., & Cahyandaru, N. (2017). Biodiversitas Kapang Pada Proses Biodeteriorasi Batuan Candi Borobudur. Borobudur, 15(2), 1–17.

Natasia, N., Jannah, S. N., & Rukmi, M. I. (2020). Potensi Antifungi Bakteri Asam Laktat dari Saluran Pencernaan Ayam Kampung terhadap Kapang Aspergillus flavus. Bioma : Berkala Ilmiah Biologi, 22(1), 91–102. https://doi.org/10.14710/bioma.22.1.91-102

Obidi, O., & Okekunjo, F. (2017). Bacterial and Fungal Biodeterioration of Discolored Building Paints in Lagos, Nigeria. World Journal of Microbiology and Biotechnology, 33(11), 1–9. https://doi.org/10.1007/s11274-017-2362-y

Poernama, J. A., & Putra, H. A. (2022). Penggunaan Bahan Batuan Berdasarkan Lokasi Terbangun: Studi Kasus Candi Di Jawa Tengah Dan Jawa Timur. ALUR : Jurnal Arsitektur, 5(2), 68–79. https://doi.org/10.54367/alur.v5i2.1941

Prescott, H. (2002). Laboratory Exercises in Microbiology. Laboratory Exercises in Microbiology, 117–124.

Qi-Wang, Ma, G. Y., He, L. Y., & Sheng, X. F. (2011). Characterization of Bacterial Community Inhabiting the Surfaces of Weathered Bricks of Nanjing Ming City Walls. Science of the Total Environment, 409(4), 756–762. https://doi.org/10.1016/j.scitotenv.2010.11.001

Rahma, A. A. (2020). Potensi Sumber Daya Alam dalam Mengembangkan Sektor Pariwisata di Indonesia. Jurnal Nasional Pariwisata, 12(April), 1–8. https://doi.org/https://doi.org/10.22146/jnp.52178

Rosselló-Móra, R., & Amann, R. (2015). Past and Future Species Definitions for Bacteria and Archaea. Systematic and Applied Microbiology, 38(4), 209–216. https://doi.org/10.1016/j.syapm.2015.02.001

Sari, I. J., Wahyuni, I., Khastini, R. O., Awaliyati, E., Susilowati, A., Utari, E., & Aryantha, I. N. P. (2021). Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum. Jurnal Biodjati, 6(2), 255–263. https://doi.org/10.15575/biodjati.v6i2.13191

Shah, M. S. (2019). Isolation, Screening and Characterization of Organic Acids Producing Bacteria from Novel Source. Global Journal for Reserch Analysis, 8(1), 1–3. https://doi.org/10.36106/GJRA

Vane-Wright, R. I. (2017). Taxonomy Methods. In Reference Module in Life Sciences (Issue June 2016, pp. 1–17). https://doi.org/10.1016/b978-0-12-809633-8.02372-4

Widarti, B. N., Wardhini, W. K., & Sarwono, E. (2015). Pengaruh Rasio C/N Bahan Baku Pada Pembuatan Kompos Dari Kubis dan Kulit Pisang. Jurnal Integrasi Proses, 5(2), 75–80. https://doi.org/http://dx.doi.org/10.36055/jip.v5i2.200

Zulaika, E., Rositawati, N., Teknologi, I., & Nopember, S. (2016). Keanekaragaman Bakteri Pelarut Fosfta Dari Kawasan Mangrove. Seminar Nasional Biodiversitas VI, September, 1–5.

Zulaika, E., Utomo, M. A. P., Pangestu, A. S., Alami, N. H., Shovitri, M., Prasetyo, E. N., Setiawan, E., Luqman, A., Dwianita Kuswytasari, N., & Irawan, C. (2021). Novel Carbonatogenic Bacterial Strain Isolated from Limestone Quarry in East Java, Indonesia to Improve Concrete Performance. Biodiversitas, 22(9), 3890–3898. https://doi.org/10.13057/biodiv/d220935

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Published

30-11-2024

How to Cite

Alifia, L., Zulaika, E., & Soeprijanto, S. (2024). Analysis of Bacterial Diversity In Temple Bricks Using Phenetic Numeric Taxonomy Method. Jurnal Biodjati, 9(2), 294–305. https://doi.org/10.15575/biodjati.v9i2.33476

Issue

Vol. 9 No. 2 (2024): November

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