In Vitro Evaluation of Green Tea Extract's Influence on Local Sheep


Ririn Siti Rahmatillah(1*), Diky Ramdani(2), Iman Hernaman(3), Anuraga Jayanegara(4)

(1) Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Sumedang, West Java, Indonesia, Indonesia
(2) Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Sumedang, West Java, Indonesia 45363, Indonesia
(3) Department of Animal Nutrition and Feed Technology, Faculty of Animal Husbandry, Universitas Padjadjaran, Jatinangor Campus, Sumedang 45363, Indonesia,  
(4) Department of Animal Nutrition and Feed Technology, Faculty of Animal Husbandry, IPB University, Bogor, 16680 Indonesia,  
(*) Corresponding Author

Abstract


In recent years, the exploration of natural supplements such as green tea extract and their potential benefits in livestock nutrition has gained considerable attention among researchers and agriculturalists. Green tea extract contains a high concentration of bioactive substances, such as polyphenols and catechins. This study aimed to explore the effects of varying doses of green tea extract on rumen fermentation parameters through an in vitro trial using sheep rumen liquids. A completely randomized experimental design was utilized to test the effect of 4 different doses of green tea extract treatments (0, 140, 280, and 560 mg/kg) on in vitro Hohenheim gas test method. The current study shows no significant effect of administering green tea extract at various doses on total gas production, total gas production per in vitro dry matter and organic matter degradability, volatile fatty acid per in vitro dry matter and organic matter degradability, methane production per inv vitro dry matter and organic matter degradability of green tea extract. Although no significant effects were observed on the investigated parameters, this study provides important insights in the understanding of the potential use of green tea extract in modulating fermentation processes in the ruminant’s stomach. In conclusion, the findings of this study underscore the impact of GTE on gas production regulation, highlighting its potential implications for gastrointestinal health

Keywords


degradability, gas production, green tea, Hohenheim gas test, in vitro, sheep.

Full Text:

PDF

References


AOAC. Animal feed. In: Horwitz W, Latimer GW, Thiex NW (editors). (2005). Official Methods of Analysis of AOAC International. Gaithersburg, MD, USA: AOAC International.

Bindes, M. M. M., Reis, M. H. M., Cardoso, V. L., & Boffito, D. C. (2019). Ultrasound-assisted extraction of bioactive compounds from green tea leaves and clarification with natural coagulants (chitosan and Moringa oleífera seeds). Ultrasonics sonochemistry, 51, 111-119. DOI: 10.1016/j.ultsonch.2018.10.014

Broucek, J. (2014). Production of methane emissions from ruminant husbandry: a review. Journal of Environmental Protection, 5(15), 1482. DOI: 10.4236/jep.2014.515141

Cerbin-Koczorowska, M., Waszyk-Nowaczyk, M., Bakun, P., Goslinski, T., & Koczorowski, T. (2021). Current view on green tea catechins formulations, their interactions with selected drugs, and prospective applications for various health conditions. Applied Sciences, 11(11), 4905. DOI: doi.org/10.3390/app11114905

Deepika, & Maurya, P. K. (2022). Health benefits of quercetin in age-related diseases. Molecules, 27(8), 2498. DOI: 10.3390/molecules27082498

Genzebu, D., & Tesfay, G. (2015). The role of bacteria in nitrogen metabolism in the rumen with emphasis of cattle. Research Journal of Agriculture and Environmental Management, 4(7), 282-290.

Getabalew, M., Alemneh, T., & Akeberegn, D. (2019). Methane production in ruminant animals: Implication for their impact on climate change. Concepts of Dairy and Veterinary Science, 2(8). DOI: 10.32474/CDVS.2019.02.000142

Jiao, P., Huang, J., Zhou, C., (2016). Rumen Fermentation, Intraruminal Distribution of Nutrients, and Methane Emissions in Ruminants. Journal of Animal Science and Biotechnology, 7(1), 65.

Khan, M. M. H., & Chaudhry, A. S. (2010). Chemical composition of selected forages and spices and the effect of these spices on in vitro rumen degradability of some forages. Asian-Australasian journal of animal sciences, 23(7), 889-900. DOI: 10.5713/ajas.2010.90442.

Kolling, G. J., Stivanin, S. C. B., Gabbi, A. M., Machado, F. S., Ferreira, A. L., Campos, M. M., ... & Fischer, V. (2022). Behavior of Holstein and Holstein-Gyr lactating cows supplemented with oregano and green tea extracts: Plant extracts for lactating cows. Journal of Veterinary Behavior, 49, 75-79. DOI: 10.1016/j.jveb.2021.11.004.

Li, S., Lo, C. Y., Pan, M. H., Lai, C. S., & Ho, C. T. (2013). Black tea: chemical analysis and stability. Food & function, 4(1), 10-18. DOI: 10.1039/C2FO30093A.

Ma, Y., Feng, Y., Song, L., Li, M., Dai, H., Bao, H., ... & Liang, Y. (2021). Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia. Animal nutrition, 7(1), 206-215. DOI: 10.1016/j.aninu.2020.06.005.

Maccarana, L., Cattani, M., Tagliapietra, F., Schiavon, S., Bailoni, L., & Mantovani, R. (2016). Methodological factors affecting gas and methane production during in vitro rumen fermentation evaluated by meta-analysis approach. Journal of animal science and biotechnology, 7, 1-12. DOI: 10.1186/s40104-016-0094-8

Makkar, H. P. (2003). Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small ruminant research, 49(3), 241-256. DOI: 10.1016/S0921-4488(03)00142-1.

McDougall, E. I. (1948). Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochemical journal, 43(1), 99.

Moss, A. R., Jouany, J. P., & Newbold, J. (2000). Methane production by ruminants: its contribution to global warming. In Annales de zootechnie (Vol. 49, No. 3, pp. 231-253). EDP Sciences. DOI: 10.1051/animres:2000119

Owens, F. N., & Basalan, M. (2016). Ruminal fermentation. Rumenology, 63-102. DOI: 10.1007/978-3-319-30533-2_3

Piñeiro-Vázquez, A. T., Canul-Solís, J. R., Alayón-Gamboa, J. A., Chay-Canul, A. J., Ayala-Burgos, A. J., Aguilar-Pérez, C. F.,& Ku-Vera, J. C. (2015). Potential of condensed tannins for the reduction of emissions of enteric methane and their effect on ruminant productivity. Archivos de Medicina Veterinaria, 47(3), 263-272.

Pourbayramian, R., Abdi-Benemar, H., Seifdavati, J., Greiner, R., Elghandour, M. M. M. Y., & Salem, A. Z. M. (2021). Bioconversion of potato waste by rumen fluid from slaughterhouses to produce a potential feed additive rich in volatile fatty acids for farm animals. Journal of Cleaner Production, 280, 124411. DOI: 10.1016/j.jclepro.2020.124411

Rahmatillah, R. S., Ramdani, D., Hernaman, I., Jayanegara, A., & Yanza, Y. R. (2024). Exploring multiple impacts of dietary tea supplements on ruminants: a meta-analysis. Advances in Animal and Veterinary Science, 12(10), 1924-1931. DOI: 10.17582/journal.aavs/2024/12.10.1924.1931

Ramdani, D., Budinuryanto, D. C., & Mayasari, N. (2020). The effect of paddy straw and concentrate containing green tea dust on performance andnutrient digestibility in feedlot lambs. Turkish Journal of Veterinary & Animal Sciences, 44(3), 668-674. DOI: 10.3906/vet-1909-10.

Ramdani, D., Chaudhry, A. S., Hernaman, I., & Seal, C. J. (2017). Comparing tea leaf products and other forages for in-vitro degradability, fermentation, and methane for their potential use as natural additives for ruminants. KnE Life Sciences, 63-71. DOI: 10.18502/kls.v2i6.1020

Ramdani, D., Jayanegara, A., & Chaudhry, A. S. (2022). Biochemical properties of black and green teas and their insoluble residues as natural dietary additives to optimize in vitro rumen degradability and fermentation but reduce methane in sheep. Animals, 12(3), 305. DOI: 10.3390/ani12030305.

Ribeiro Pereira, L. G., Machado, F. S., Campos, M. M., Guimaraes Júnior, R., Tomich, T. R., Reis, L. G., & Coombs, C. (2015). Enteric methane mitigation strategies in ruminants: a review. Revista Colombiana de Ciencias Pecuarias, 28(2), 124-143.

Van Soest, P. J. (1973). Collaborative study of acid-detergent fiber and lignin. Journal of the Association of Official Analytical Chemists, 56(4), 781-784. DOI: 10.1093/jaoac/56.4.781.

Van Soest, P. V., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of dairy science, 74(10), 3583-3597. DOI: 10.3168/jds.S0022-0302(91)78551-2.

Vastolo, A., Matera, R., Serrapica, F., Cutrignelli, M. I., Neglia, G., Kiatti, D. D., & Calabrò, S. (2022). Improvement of rumen fermentation efficiency using different energy sources: in vitro comparison between Buffalo and cow. Fermentation, 8(8), 351. DOI: 10.3390/fermentation8080351

Wang, L., Zhang, G., Li, Y., & Zhang, Y. (2020). Effects of high forage/concentrate diet on volatile fatty acid production and the microorganisms involved in VFA production in cow rumen. Animals, 10(2), 223. DOI: 10.3390/ani10020223

Yang, C. S., Zhang, J., Zhang, L., Huang, J., & Wang, Y. (2016). Mechanisms of body weight reduction and metabolic syndrome alleviation by tea. Molecular nutrition & food research, 60(1), 160-174. DOI: 10.1002/mnfr.201500428.

Zhong, R. Z., Li, H. Y., Fang, Y., Sun, H. X., & Zhou, D. W. (2015). Effects of dietary supplementation with green tea polyphenols on digestion and meat quality in lambs infected with Haemonchus contortus. Meat Science, 105, 1-7. DOI: 10.1016/j.meatsci.2015.02.003

Zhong, R. Z., Li, H. Y., Sun, H. X., & Zhou, D. W. (2014). Effects of supplementation with dietary green tea polyphenols on parasite resistance and acute phase protein response to Haemonchus contortus infection in lambs. Veterinary Parasitology, 205(1-2), 199-207. DOI: 10.1016/j.vetpar.2014.06.022




DOI: https://doi.org/10.15575/biodjati.v9i2.34573

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Jurnal Biodjati

License URL: https://creativecommons.org/licenses/by-nc-nd/4.0/

Indexing By :

      

      

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

View My Stats