Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.)

Authors

  • Farah Aldila Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292
  • Ari Susilowati Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292
  • Ratna Setyaningsih Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292

DOI:

https://doi.org/10.15575/biodjati.v4i2.4525

Keywords:

biodegradation, polyurethane, teak wood

Abstract

Polyurethane is a class of polymers characterized by their substantial tensile strength and high melting points which enables them to be extremely durable. Despite its xenobiotic origin, polyure-thane has been found susceptible to biodegradation by microorgan-isms. The main object of this research was to investigate polyurethane degradation by the bacterium isolated from decayed teak wood (Tec-tona grandis Linn. f.). Polyurethane degrading bacteria were isolated using selective medium in the form of LB (Luria Bertani) with the ad-dition of 0.3% Impranil. Bacterial biodegradation activities shown by the changes of polyurethane structure analyzed by FT-IR spectrosco-py. The bacterial identification was carried out based on observations on the morphological characteristics of the colonies, cell morphology and sequences of 16S rRNA encoding genes. One of the potential iso-late that successfully isolated was K9, which demonstrates the disap-pearance of the 1735/cm peak of the characteristic function urethane in the FT-IR analysis. Analysis of the 16S rRNA encoding gene showed that the potential isolate having 98% similarity index to Bacillus safen-sis strain FO-36b. The activity shown by the isolate suggests that the bacteria could be a promising agent for polyurethane degradation. 

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Published

2019-11-29

Issue

Vol. 4 No. 2 (2019): November

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