Effects of Herring Fillets and By-Products on Protein Digestibility, Hematology, and Blood Biochemistry in Rats
DOI:
https://doi.org/10.15575/biodjati.v10i2.45391Keywords:
blood biochemistry, clupea harengus, fish by-products, hematology, protein digestibility, smoking processingAbstract
Protein bioavailability and digestibility are critical for animal growth and health and are influenced by source and processing methods. This study investigated the effects of diets incorporating processed Clupea harengus (herring) fillets and by-products (heads, bones, viscera) prepared by charcoal, wood, or poaching on growth performance, protein quality, hematology, and blood biochemistry in Wistar rats. Forty rats were randomly allocated to eight dietary groups: charcoal-smoked fillet (CSFBD), wood-smoked fillet (WSFBD), poached fillet (PFD), charcoal-smoked by-products (CSHB), wood-smoked by-products (WSHB), poached by-products (PSF), soybean-based positive control, and basal negative control. Diets were fed for 28 days. Smoked by-product diets (CSHB and WSHB) produced significantly higher final body weights (120.86 ± 0.68 g and 105.52 ± 19.94 g) and weight gains (9.14 ± 1.39 g, p ≤ 0.05) than the basal diet (59.55 ± 1.37 g and 5.35 ± 0.25 g). Smoked diets improved feed intake, feed conversion ratio, apparent protein digestibility, and biological value. Hematological parameters remained within physiological ranges across groups; the soybean diet showed the highest RBC (7.8 × 10¹²/L) and hemoglobin (16.3 g/dL), whereas smoked fish diets recorded slightly lower but normal values (RBC 7.0-7.5 × 10¹²/L; hemoglobin 14-15 g/dL). Biochemical profiles revealed that smoked diets reduced serum triglycerides and creatinine, suggesting enhanced lipid metabolism and renal function. Smoking significantly improves the nutritional quality of Clupea harengus fillets and by-products, making them practical, safe, and growth-promoting protein sources for animal nutrition. Inclusion of smoked fish by-products offers a sustainable alternative to conventional plant proteins while supporting metabolic and systemic health
References
Adeyemi, O. T., Osilesi, O., Adebawo, O., Onajobi, F. D., & Oyedemi, S. O. (2013). Stability study of smoked fish, horse mackerel (Trachurus trachurus), by different methods and storage at room temperature. African Journal of Biochemistry Research, 7(6), 98–106. DOI: 10.5897/AJBR2013.0672
Adeyemi, O. T., Osilesi, O., Adebawo, O. O., Onajobi, F., Oyedemi, S., & Afolayan, A. J. (2015). Influence of processing methods on protein quality of Atlantic horse mackerel (Trachurus trachurus). Advances in Life Science and Technology, 30, 20–33.
Adeyemi, O. T., Osilesi, O., Adebawo, O. O., Onajobi, F., Oyedemi, S., & Afolayan, A. J. (2015). Selected lipid profile in the serum & tissues of weaned male albino rats fed on processed Atlantic horse mackerel (Trachurus trachurus). Advances in Bioscience and Biotechnology, 6(4), 286–301. DOI: 10.4236/abb.2015.64029
Adeyemi, O. T., Osilesi, O., Adebawo, O. O., Onajobi, F. D., Oyedemi, S. O., & Afolayan, A. J. (2015). Evaluation of the levels of reactive di-carbonyl compounds and polyaromatic hydrocarbons in processed Atlantic horse mackerel (Trachurus trachurus). African Journal of Science, Technology, Innovation and Development, 7(2), 84–91. DOI: 10.1080/20421338.2015.1010634
Aiello, P. (2025). Future-proofing the manufacturing workforce: The role of industry in education. Quality, 64(9), 33.
Association of Official Analytical Chemists (AOAC). (2002a). Moisture content in meat. In Official methods of analysis (17th ed., pp. 950.46). Gaithersburg, MD: AOAC International.
Association of Official Analytical Chemists (AOAC). (2002b). Official method 928.08 – Nitrogen in meat and Protein content in meat. In Official methods of analysis (17th ed., p. 928.08). Gaithersburg, MD: AOAC International.
Association of Official Analytical Chemists (AOAC). (2002c). Official method 960.39 – Fat content in meat. In Official methods of analysis (17th ed., p. 960.39). Gaithersburg, MD: AOAC International.
Association of Official Analytical Chemists (AOAC). (2002d). Official method 920.153 –Ash content. In Official methods of analysis (17th ed., p. 920.153). Gaithersburg, MD: AOAC International.
Association of Official Analytical Chemists (AOAC). (1984). Official methods of analysis (14th ed.). Washington, DC: AOAC.
AOAC. (2003). Official methods of analysis (17th ed.). Gaithersburg, MD: AOAC International.
Brien, R. O., Hayes, M., Sheldrake, G., Tiwari, B., & Walsh, P. (2022). Macroalgal proteins: a review. Foods, 11(4), 571.
Calder, P. C. (2015). Marine omega-3 fatty acids and inflammatory processes: Effects, mechanisms and clinical relevance. Biochimica et Biophysica Acta – Molecular and Cell Biology of Lipids, 1851(4), 469–484. DOI: 10.1016/j.bbalip.2014.08.010
Chalamaiah, M., Dinesh Kumar, B., Hemalatha, R., & Jyothirmayi, T. (2012). Fish protein hydrolysates: Proximate composition, amino acid composition, antioxidant activities and applications: A review. Food Chemistry, 135(4), 3020–3038. DOI: 10.1016/j.foodchem.2012.06.100
Damodaran, S., & Parkin, K. L. (2017). Fennema’s food chemistry (5th ed.). CRC Press.
Elgaoud, I., Hamed, F., Lassoued, I., Chamkha, M., Oulahal, N., Degraeve, P., & Barkia, A. (2024). Production of hydrolysates from swordfish (Xiphias gladius) head muscle as new protein source: evaluation of nutritional, antioxidant and functional properties. Waste and Biomass Valorization, 15(2), 1065-1080.
FAO. (2022). The State of World Fisheries and Aquaculture 2022: Towards Blue Transformation. Rome: FAO. DOI: 10.4060/cc0461en
FAO/WHO. (1991). Protein quality evaluation: Report of joint FAO/WHO expert consultation. FAO Food and Nutrition Paper 51. Rome: FAO.
FAO/WHO/UNU/WHO. (2007). Protein and amino acid requirements in human nutrition: Report of a joint FAO/WHO/UNU expert consultation. WHO Technical Report Series No. 935. Geneva: World Health Organization.
FAO/WHO/UNU. (1985). Energy and protein requirements: Report of a joint FAO/WHO/UNU expert consultation. WHO Technical Report Series No. 724. Geneva: World Health Organization.
Gonzalez Martinez, A., De-Pablos-Heredero, C., González, M., Rodriguez, J., Barba, C., & García, A. (2021). Morphological variations of wild populations of Brycon dentex (Characidae, Teleostei) in the Guayas hydrographic basin (Ecuador). Animals, 11(7), 1901. DOI: 10.3390/ani11071901
Jiang, F., Huang, X., Chen, X., & Xian, Y. (2025). Hepatorenal protective effects of essential oils against chemical overexposure induced oxidative damage. Frontiers in Pharmacology, 16, Article 1580805. DOI: 10.3389/fphar.2025.1580805
Johnson, M. L., & Parsons, C. M. (1997). Effects of raw material source, ash content, and assay length on protein efficiency ratio and net protein ratio values for animal protein meals. Poultry Science, 76(12), 1722–1727. DOI: 10.1093/ps/76.12.1722
Lee, S., Park, C. O., Choi, W., Bae, J., Kim, J., Choi, S., & Bai, S. C. (2022). Partial substitution of fish oil with microalgae (Schizochytrium sp.) can improve growth performance, nonspecific immunity, and disease resistance in rainbow trout, Oncorhynchus mykiss. Animals, 12(9), Article 1220. DOI: 10.3390/ani12091220
Li, X., Rezaei, R., Li, P., & Wu, G. (2022). Composition of amino acids in foodstuffs for humans and animals. Advances in Experimental Medicine and Biology, 1332, 1–22. DOI: 10.1007/978-3-030-74180-8_1
Nwokedi, V. U., Ogadah, C. O., Adeyemi, B. I., Iregbu, J. K., Felix, J. C., Enechukwu, E. O., ... & Sone, P. E. (2025). A review on safety and public health implications of use of over-the-counter pain medication. Journal of Pharma Insights and Research, 3(2), 194–202. DOI: 10.69613/7maz7058
Reinik, M., Tamme, T., Roasto, M., Juhkam, K., Tenno, T., & Kiis, A. (2007). Polycyclic aromatic hydrocarbons (PAHs) in meat products and estimated PAH intake by children and the general population in Estonia. Food Additives & Contaminants, 24(4), 429–437. DOI: 10.1080/02652030601182862
Santos, F. A., Batista, F. S., Souza, A. S., Julio, G. S., Favero, G. C., Junior, J. F., & Luz, R. K. (2023). Growth performance and histomorphology of intestine, skin, gills, and liver of juvenile Colossoma macropomum fed diets containing different levels of the essential oil of Nectandra grandiflora. Fishes, 8(10), Article 509. DOI: 10.3390/fishes8100509
Sarwar, G., & Peace, R. W. (1986). Comparisons between true digestibility of total nitrogen and limiting amino acids in vegetable proteins fed to rats. The Journal of Nutrition, 116(7), 1172–1184. DOI: 10.1093/jn/116.7.1172
Singh, A., Mittal, A., & Benjakul, S. (2022). Undesirable discoloration in edible fish muscle: Impact of indigenous pigments, chemical reactions, processing, and its prevention. Comprehensive Reviews in Food Science and Food Safety, 21(1), 580–603. DOI: 10.1111/1541-4337.12866
Talabi, O. T., Ademilua, T. E., Akande, T. O., Oluwole, R. A., Osilesi, O., Adebawo, O. O., & Talabi, M. J. (2023). Brewing health benefits: A comparative study of black, herbal, and green tea on rat physiology. Shodh Sari, 2(4), 28–48. https://icertpublication.com/index.php/shodh-sari-2/sodha-sari-vol-2-issue-4/
Tolasa, S., Cakli, S., & Ostermeyer, U. (2019). Determination of the effect of smoking methods on protein digestibility and quality of smoked rainbow trout (Oncorhynchus mykiss). Journal of Aquatic Food Product Technology, 28(5), 517–526. DOI: 10.1080/10498850.2019.1617672
Wang, C., Jiang, C., Gao, T., Peng, X., Ma, S., Sun, Q., & Zhuang, X. (2022). Improvement of fish production and water quality in a recirculating aquaculture pond enhanced with bacteria-microalgae association. Aquaculture, 547, Article 737420. DOI: 10.1016/j.aquaculture.2021.737420
Witeska, M., Kondera, E., & Bojarski, B. (2023). Hematological and hematopoietic analysis in fish toxicology – A review. Animals, 13(16), Article 2625. DOI: 10.3390/ani13162625
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