Comparison of Quality and Ethanol Content of Liquid and Powder Kombucha from Jasmine Tea (Jasminum sambac L.)
DOI:
https://doi.org/10.15575/biodjati.v10i2.47357Keywords:
ethanol, gum arabic, kombucha, maltodextrin, powderAbstract
The fermentation process of kombucha has become a focus of halal product research due to its ethanol content. Processing kombucha into powder form can be an alternative to address the ethanol content in liquid kombucha, as it has undergone a drying process, making it a potential halal-certified functional drink. The objective of this study was to compare the quality of liquid and powdered kombucha. The research was conducted from March to September 2024 at the Biotechnology Laboratory, Muhammadiyah University of Bandung. The method used was the Complete Randomized Design (CRD) method. The parameters analyzed included ethanol content, total BAA (Bacterial Acetic Acid), pH, organic acid content, and sugar content. In the production of powdered kombucha, optimization of maltodextrin and gum arabic usage was also conducted to achieve the best solubility and taste. The best powdered kombucha was obtained with a maltodextrin-to-gum arabic ratio of 3:2. In the research, liquid kombucha contained ethanol at an average of 0.09%. In comparison, no ethanol was detected in the powdered kombucha (0%). Total BAA in powder decreased after the drying process (p<0.05). The pH value of kombucha in liquid form (4.4) also differed significantly from powdered (3.2) (p<0.05). However, the levels of organic acids and sugars in liquid and powdered kombucha did not differ significantly (p>0.05). The sugar content in liquid and powdered kombucha was lower than that in kombucha in general.
References
Holt, J. G. (1994). Bergey’s manual of determinative bacteriology (9th ed.). The William & Wilkim Co.Inc.
Ihsani, N., Hernahadini, N., & Fauzi, M. (2021). The variation of ethanol concentration and kombucha characterization on several incubation periods. Journal of Physics: Conference Series, 1764(1). DOI: 10.1088/1742-6596/1764/1/012008
Ihsani, N., Hernahadini, N., Nurissalma, Z., Huda, A. N., Sartika, T., Afifah, A. N. N., & Syamsiyah, N. R. N. (2023). The effect of incubation period on ethanol content of jasmine and green tea kombucha. Jurnal Bioteknologi & Biosains Indonesia, 10(October), 361–367. http://ejournal.brin.go.id/JBBI/index
Jakubczyk, K., Kaldunska, J., Kochman, J., & Janda, K. (2020). Chemical profile and antioxidant activity of the kombucha beverage derived from white, green, black and red tea. Antioxidants, 9(5). DOI: 10.3390/antiox9050447
Jasman, J., Prijambada, I. D., Hidayat, C., & Widianto, D. (2015). Selection of Yeast Strains for Ethanol Fermentation of Glucose Fructose Sucrose Mixture. Indonesian Journal of Biotechnology, 17(2), 114. DOI: 10.22146/ijbiotech.16001
Keller, J., Kahlhofer, J., Peter, A., & Bosy-Westphal, A. (2016). Effects of low versus high glycemic index sugar-sweetened beverages on postprandial vasodilatation and inactivity-induced impairment of glucose metabolism in healthy men. Nutrients, 8(12), 1–14. DOI: 10.3390/nu8120802
Khan, A. A., Ali, S. W., Manzoor, S., Ayub, S. R., & Ilyas, M. (2016). Influence of sugar concentration on physicochemical properties and sensory attributes of sapodilla jam. PeerJPreprints, 1–10. DOI: 10.7287/peerj.preprints.1777v1
Majidah, L., Gadizza, C., & Gunawan, S. (2022). Analisis Pengembangan Produk Halal Minuman Kombucha. Halal Research Journal, 2(1), 36–51. DOI: 10.12962/j22759970.v2i1.198
Miranda, J. F., Ruiz, L. F., Silva, C. B., Uekane, T. M., Silva, K. A., Gonzalez, A. G. M., Fernandes, F. F., & Lima, A. R. (2022). Kombucha: A review of substrates, regulations, composition, and biological properties. Journal of Food Science, 87(2), 503–527. DOI: 10.1111/1750-3841.16029
Munjal, S., & Aakash, S. (2020). The Arrhenius Acid and Base Theory. In Intech. InTechOpen.
Mursalin, M., Nizori, A., & Rahmayani, I. (2019). The Effect of Heating Schedule on PhysicoChemical Properties of Instant Coffee of Liberika Tungkal Jambi. Indonesian Food Science and Technology Journal, 2(2), 26–29. DOI: 10.22437/ifstj.v2i2.9442
Naibaho, N. M., Munthe, S., Popang, E. G., and Zamroni, A. (2019). Uji sensoris minuman kulit buah naga (Hylocereus costaricensis). Buletin LOUPE, 15(1), 24–30.
Neffe Skocinska, K., Sionek, B., Scibiszban, I., and Kolozyn-Krajewska, D. (2017). Acid contents and the effect of fermentation condition of Kombucha tea beverages on physicochemical, microbiological and sensory properties. CYTA-Journal Food, 15(7), 601–607.
Ningtyas, R. N. (2015). Pengaruh lama fermentasi dan jumlah inokulum terhadap karakteristik kimia dan potensi antibakteri teh kombucha dari air rebusan jagung manis (Zea mays saccharata Sturt). Universitas Islam Negeri Maulana Malik Ibrahim Press.
Nyhan, L. M., Lynch, K. M., Sahin, A. W., & Arendt, E. K. (2022). Advances in Kombucha Tea Fermentation: A Review. Applied Microbiology, 2(1), 73–103. DOI: 10.3390/applmicrobiol2010005
Pirestani, S., Nasirpour, A., Keramat, J., Desobry, S., & Jasniewski, J. (2017). Effect of glycosylation with gum Arabic by Maillard reaction in a liquid system on the emulsifying properties of canola protein isolate. Carbohydrate Polymers, 157, 1620–1627.
Puspaningrum, D. H. D, Sumadewi, N. L. U., and Sari, N. K. Y. (2021). Kandungan total asam, total gula dannilai pH kombucha cascara kopi arabika Desa Catur Bangli selama fermentasi. In Prosiding SINTESA.
Putu, A. (2018). Buku panduan membuat teh kombucha di rumah. Wikikombucha.
Rizki, Z., Fitriana, F., and Jumadewi, A. (2022). Identifikasi jumlah angka kuman pada dispenser metode TPC (Total Plate Count). Jurnal SAGO Gizi Dan Kesehatan, 39–43(1), 3.
Rosida, D. F., Sofiyah, D. L., & Putra, A. Y. T. (2021). Aktivitas antioksidan minuman serbuk kombucha dari daun ashitaba (Angelica keiskei), kersen (Muntingia calabura), dan kelor (Moringa oleifera). Jurnal Teknologi Pangan, 15(1), 81–97.
Santoso, B., Sarungallo, Z. L., and Dewi, A. M. P. (2019). Pengaruh suhu hidrolisis terhadap sifat fisiko-kimia maltodekstrin dari pati sagu. In Prosiding SeminarNasional Penelitian & Pengabdian Kepada Masyarakat.
Savitri, D. A., Setiyono., Meliala, S. B. P. S., Arum, A. P., Novijanto, N., and Herliani, C. P. (2024). Karakteristik Kimia dan Mikrobiologi Minuman Kombucha yang Diproduksi dari Kopi Robusta dan Arabika dengan Profil Sangrai yang Beragam. Jurnal Ilmu Dan Teknologi Pertanian Terapan, 8(3), 290–302.
Shah, N. P. (2007). Functional cultures and health benefits. International Dairy Journal, 17(11), 1262–1277.
Su, W., Jiang, Y., Zuo, X., Li, C., & Honghai, W. (2022). Engineering nucleation/crystallization to intensify the enzymatic reactions and fermentation: a review. Chemical Engineering Journal, 431(2).
Villarreal Soto S. A., Beaufort S., B., & J., Souchard J., T. P. (2018). No Title. Understanding Kombucha Tea Fermentation: A Review, 83(3), 580–588. DOI: 10.1111/1750-3841.14068
Wang, P., Feng, Z., Sang, X., Chen, W., Zhang, X., Xiaou, J., Chen, Y., Chen, Q., Yang, M., and Su, J. (2021). Kombucha ameliorates LPS-induced sepsis in a mouse model. Food Funct, 19(20), 10263–10280.
Wati, R., and Sutiadiningsih, A. (2016). Pengaruh penambahan carboxy methyl cellulose (cmc) dan asam sitrat terhadap mutu produk sirup belimbing manis (Averrhoa carambola). E-Journal Boga, 3(2), 54–62.
Yuliani N, Maslahat M, L. P. (2017). Optimasi Waktu Pengeringan Terhadap Aktivitas Antioksidan Ekstark Etanol Rimpang Temu Putih (Curcuma aromatica salisb). Jurnal Sains Natural, 4(2), 143–147.
Downloads
Published
How to Cite
Issue
Section
Citation Check
License
Copyright (c) 2025 Jurnal Biodjati

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright and Attribution:
Copyright of published in Jurnal Biodjati is held by the journal under Creative Commons Attribution (CC-BY-NC-ND) copyright. The journal lets others distribute and copy the article, create extracts, abstracts, and other revised versions, adaptations or derivative works of or from an article (such as an tranlation), include in collective works (such as an anrhology), text or data mine the article, as long as they credit the author(s), do not represent the author as endorsing their adaptation of the article and do not modify the article in such a way as to damage the author's honor or reputation.
Permissions:
Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate of one of the authors.
Ethical matters:
Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the authors own must have had the correct authorization for this exportation.
















