Actinomycetes from the Soil of Chilli Plantation in Yogyakarta Showing an Antagonism to Fusarium oxysporum FU3

Liya Audinah, Miftahul Ilmi

Abstract


 Application of biocontrol agent is an environmental-ly-friendly method to overcome the Fusarium wilt in chilli due to Fusarium oxysporum. This research aimed to isolate actinomycetes antagonist to F. oxysporum FU3 as biocontrol agent from the soil of chilli plantation in Kulon Progo, Yogyakarta. The actinomycetes were isolated using SCA medium and followed by screening for anti-F. oxysporum FU3 on PDA. The filtrate from isolates which showing the highest inhibition zone was produced and tested against strain FU3. The best isolate was then characterized. There are four iso-lates of actinomycetes showing antagonistic activity against F. ox-ysporum (AK4, AK5, AK7, and AK9). The highest antagonistic ac-tivity was shown by AK5 filtrate with inhibitions on PDA and PDB are 41.71 ± 3.5 % and 81.77 ± 5.1 %, respectively. Further obser-vation showed that filtrate of AK5 caused abnormality and lysis on FU3 hyphae. From the results, we concluded that AK5 is a poten-tial biocontrol agent against F. oxysporum. Further studies to opti-mize the production and formulize the bio-fungicide are suggested 


Keywords


biocontrol; biofungicide; fusarium wilt; antagonist; isolation; screening

Full Text:

PDF

References


Anandan, R., Dharumadurai, D. & Manoga-ran, G. P. (2016). An Introduction to Ac-tinobacteria. Intechopen.

Asif, M. (2017). Review on Antimicrobial Agents. Organic and Medicinal Chem International Journal, 1(5), 1-7.

Dias, I. J., Trajanoa, E. R. I. S., Castrob, R. D., Ferreirab, G. L. S., Medeirosc, H. C. M. & Gomes, D. Q. C. (2018). Antifungal activity of Linalool in Cases of Candida spp. Isolated from Individuals with Oral Candidiasis. Brazilian Journal of Biolo-gy, 78(2), 368-374.

Gopinath, L. R., Premalatha, K., Jothi, G., Archaya. S., Rajamuni, P. & Suresh, K. B. T. (2018). Isolation and Screen-ing of Effective Antibiotic Producing actinomycetes from rhizosphere soil of Cipadessa baccifera and Clausena den-tata. Journal of Biotechnology and Bio-chemistry, 4(5), 39-47.

Kavitha, K., Akila, A., Nandhini, A. & Shakunthala, P. (2014). Quorum Sens-ing: A Review. Indian Journal of Multi-disciplinary Dentistry, 4(3), 970-974.

Koley, S. & Mahapatra, S. S. (2015). Evalua-tion of Culture Media for Growth Char-acteristics of Alternaria solani, Causing Early Blight of Tomato Article. Journal of Plant Pathology and Microbiology, 1(5), 1-5.

Kong, D., Lee, M. J., Lin, S. & Kim E. S. (2013). Review : Biosynthesis and Path-way Engineering of Antifungal Polyene Macrolides in Actinomycetes. Journal of Industrial Microbiology and Biotech-nology, 40(6), 529-543.

Krzesniak, K. J., Mateusiak, A. R., Guspiel, A., Ziemska, J. & Soleck, J. (2018). Secondary Metabolites of Actinomycet-es and Their Antibacterial, Antifungal and Antiviral Properties. Polish Journal of Microbiology, 67(3), 259–272.

Li, Q., Chen, X., Jiang, Y. & Jiang, C. (2016). Morphological Identification of Actino-bacteria. Intechopen.

Ministry of Agriculture RI (2016). Outlook Cabai. Ministry of Agriculture, Repub-lic of Indonesia. Jakarta. hal 16-45.

Mona, M. M., Ashour, A. M. A., Kader, M. M. A., El-Mohamady, R. & Aziz, A. A. (2012). In vitro Evaluation of some Fungicides Alternatives Against Fusar-ium oxysporum the Causal of Wilt Dis-ease of Pepper (Capsicum annum L.). International Journal of Agriculture and Forestry, 2(2), 70-77.

Raja, A. & Prabalarana, P. (2011). Actinomy-cetes and Drug : An Overview. Ameri-can Journal of Drug Discovery and De-velopment, 1(2), 72-84.

Sari, E. M., Suwirmen & Noli, Z. A. (2014). Pengaruh Penggunaan Fungisida (Dith ane M-45) terhadap Pertumbuhan Tan-aman Jagung (Zea mays L.) dan Kepa-datan Spora Fungi Mikoriza Arbuskula (FMA). Jurnal Biologi Universitas An-dalas, 3(3), 188-194.

Thompson, G. R., Cadena, J. & Patterson, T. F. (2009). Overview of Antifungal Agents. Clinics in Chest Medicine, 30, 203-225.

Vurukonda, S. S. K. P., Giovanardi, D. & Ste-fani, E. (2018). Plant Growth Promoting and Biocontrol Activity of Streptomyces spp. as Endophytes. International Jour-nal of Molecular Sciences, 19(952), 1-26.

Wang, Qi, Duan, B., Yang, R., Zhao, Y. & Zhang, L. (2015). Screening and Iden-tification of Chitinolytic Actinomycet-es and Study on the Inhibitory Activi-ty Against Turfgrass Root Rot Disease Fungi. Journal of Biosciences and Med-icines, 1, 56-65.

Zhao, Y., Selvaraj, J. N., Xing, F., Zhou, L., Wang, Y., Song, H., Tan, X., Sun, L., Sangare, L., Folly, Y. M. E. & Liu, Y. (2014). Antagonistic Action of Bacil-lus subtilis Strain SG6 on Fusarium graminearum. PLoS ONE, 9(3), 1-11.




DOI: https://doi.org/10.15575/biodjati.v4i2.5237

Refbacks

  • There are currently no refbacks.


Indexing By :

      

      

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

View My Stats