Dietary Streptococcus thermophilus Probiotic Improves Growth of Pacific White Shrimp and Nile Tilapia
DOI:
https://doi.org/10.15575/biodjati.v11i1.55065Keywords:
aquaculture, efficiency, growth, nutrition, probioticsAbstract
The intensive culture of Pacific white shrimp (Litopenaeus vannamei) and Nile tilapia (Oreochromis niloticus) is constrained by high feed costs, underscoring the need for strategies that enhance growth and feed efficiency without compromising environmental quality. Probiotics are increasingly proposed as functional feed additives, yet the performance of Streptococcus thermophilus in these two key tropical species remains poorly documented. This study evaluated the effects of graded dietary levels of S. thermophilus on growth performance, feed utilization, survival, and water quality in shrimp and Nile tilapia reared under controlled hatchery conditions. Shrimp (PL‑15) and Nile tilapia juveniles (4–6 cm) were cultured separately in 25‑L plastic tanks (effective water volume 15–16 L), at densities of 50 shrimp tank⁻¹ (31–32 ppt) and 15 tilapia tank⁻¹ (10–15 ppt), respectively, for 40 days. A completely randomized design with five treatments (0, 5, 10, 15, and 20 mL S. thermophilus/kg feed) and three replicates per treatment was used, with isoproteic diets based on fish meal and plant ingredients. Growth parameters (absolute weight and length gain, specific growth rate), feed conversion ratio, feed efficiency, survival, and basic water quality variables were measured and analyzed by one‑way ANOVA followed by Duncan’s test. Dietary S. thermophilus significantly enhanced absolute weight gain and specific growth rate in both shrimp and tilapia, while absolute length gain remained unchanged (P > 0.05). The 15 mL/kg treatment produced the best overall performance, with shrimp and tilapia reaching the highest weight gain, lowest feed conversion ratios (1.26 and 1.20, respectively), and feed efficiencies above 79%, whereas survival exceeded 70% in all probiotic groups and reached 100% in some tilapia treatments. Water quality, including dissolved oxygen, temperature, and pH, remained within recommended ranges, and probiotic pH (2–4) reflected active lactic acid fermentation without detrimental effects on rearing conditions. These findings demonstrate that dietary S. thermophilus at 10–15 mL/kg is an effective strategy to improve growth and feed utilization in shrimp–tilapia systems while maintaining suitable water quality, offering a practical approach to reduce feed-related production costs in sustainable tropical aquaculture.
References
Abbas, M. A., Alamanda, N., Sahidir, I., Damora, A., Devira, C. N., & Putra, D. F. (2023). The application of yeast and lactic acid bacteria improve growth and intestinal structure of tiger shrimp larvae Penaeus monodon Fab. Jurnal Kedokteran Hewan – Indonesian Journal of Veterinary Sciences, 17(1): 35-41. DOI: https://doi.org/10.21157/j.ked.hewan.v17i1.30379
Abbas, M. A., Hababil, M., Putra, D. F., Wahab, G. A., & Devira, C. N. (2025). Periodic starvation provokes the growth performance and feed efficiency of jelawat fish (Leptobarbus hoevenii). BIO Web of Conferences, 156: 03041. DOI: https://doi.org/10.1051/bioconf/202515603040
Abd El Hack, M. E., El Saadony, M. T., Nader, M. M., Salem, H. M., El Tahan, A. M., Soliman, S. M., & Khafaga, A. F. (2022). Effect of environmental factors on growth performance of Nile tilapia (Oreochromis niloticus). International Journal of Biometeorology, 66(11). DOI: https://doi.org/10.1007/s00484-022-02347-6
Abdul Malik, A., Aliyu Paiko, M., Adamu, K. M., Aliyu A, A., & Mohammed, J. N. (2023). Role of prebiotic, probiotic and symbiotic diets on bacterial proliferation in feed and intestine of African catfish (Clarias gariepinus). Journal of Applied Sciences and Environmental Management, 27(1): 87–93. DOI: https://doi.org/10.4314/jasem.v27i1.13
Afrilasari, W., Widanarni, & Meryandini, A. (2016). Effect of probiotic Bacillus megaterium PTB 1.4 on the population of intestinal microflora, digestive enzyme activity and the growth of catfish (Clarias sp.). HAYATI Journal of Biosciences, 23(4): 168-172. DOI: https://doi.org/10.1016/j.hjb.2016.12.005
Akter, M. N., Hashim, R., Sutriana, A., Siti Azizah, M. N., & Asaduzzaman, M. (2019). Effect of Lactobacillus acidophilus supplementation on growth performances, digestive enzyme activities and gut histomorphology of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) juveniles. Aquaculture Research, 50(3): 786–797. DOI: https://doi.org/10.1111/are.13938
Alifia, D., Putra, D. F., Nurfadillah, & Suratno. (2025). Administration of a combined probiotic feed containing Streptococcus thermophilus and Bacillus coagulans on growth performance and molting frequency in black tiger shrimp (Penaeus monodon Fab.). Journal of Vocational in Aquaculture (JAVA), 1(2): 8-14. DOI: https://doi.org/10.29244/java.v1i2.61537
Amelia, F., Yustiati, A., & Andriani, Y. (2021). Review of shrimp (Litopenaeus vannamei) farming in Indonesia: Management operating and development. World Scientific News, 158: 145–158.
Amenyogbe, E. (2023). Application of probiotics for sustainable and environment friendly aquaculture management: A review. Cogent Food and Agriculture, 9(1). DOI: https://doi.org/10.1080/23311932.2023.2226425
Arisa, I. I., Muchlisin, Z. A., Purba, S., Muhammadar, M., & Mellisa, S. (2021). The effect of papain and bromelain enzymes on the growth and feed utilization of post larvae Litopenaeus vannamei. IOP Conference Series: Earth and Environmental Science, 674(1): 012097. DOI: https://doi.org/10.1088/1755-1315/674/1/012097
Arisa, I. I., Widanarni, Yuhana, M., Muchlisin, Z. A., & Muhammadar, A. A. (2015). The application of probiotics, prebiotics and synbiotics to enhance the immune responses of vannamei shrimp (Litopenaeus vannamei) to Vibrio harveyi infection. AACL Bioflux, 8(5): 772–778.
Aviz, M. A. B., Maciel, C. M. T., Cordeiro, C. A. M., Fugimoto, R. Y., & Maciel, C. R. (2021). Incorporation of probiotic bacteria on larval culture of Macrobrachium amazonicum. Research, Society and Development, 10(14). DOI: https://doi.org/10.33448/rsd-v10i14.21721
Ayyat, M. S., Labib, H. M., & Mahmoud, H. K. (2014). A probiotic cocktail as a growth promoter in Nile tilapia (Oreochromis niloticus). Journal of Applied Aquaculture, 26(3). DOI: https://doi.org/10.1080/10454438.2014.934164
Azizah, F. A., Lunggani, A. T., & Laily, N. (2025). Karakteristik mikrobiologis, kimiawi dan profil asam amino dari sari kacang koro pedang (Canavalia ensiformis) yang difermentasi menggunakan symbiotic culture of bacteria and yeast (SCOBY). Jurnal Teknologi Pangan, 9(1). DOI: https://doi.org/10.14710/jtp.2025.44237
Boyd, C. E., D’Abramo, L. R., Glencross, B. D., Huyben, D. C., Juarez, L. M., Lockwood, G. S., McNevin, A. A., Tacon, A. G. J., Teletchea, F., Tomasso, J. R., Tucker, C. S., & Valenti, W. C. (2020). Achieving sustainable aquaculture: Historical and current perspectives and future needs and challenges. Journal of the World Aquaculture Society, 51(3). DOI: https://doi.org/10.1111/jwas.12714
Dachi, A. L., Muhammadar, A. A., Sahidhir, I., Putra, D. F., & Irwan, Z. A. (2019). Effects of probiotics (Rabal) with different doses on the survival, feed conversion, and growth of giant prawns (Macrobrachium rosenbergii). IOP Conference Series: Earth and Environmental Science, 348(1): 012083. DOI: https://doi.org/10.1088/1755-1315/348/1/012083
De Marco, G., Cappello, T., & Maisano, M. (2023). Histomorphological changes in fish gut in response to prebiotics and probiotics treatment to improve their health status: A review. Animals, 13(18). DOI: https://doi.org/10.3390/ani13182860
Dias, D. C., Tachibana, L., Iwashita, M. K. P., Nakandakare, I. B., Romagosa, E., Seriani, R., & Ranzani Paiva, M. J. T. (2020). Probiotic supplementation causes hematological changes and improves non specific immunity in Brycon amazonicus. Acta Scientiarum. Biological Sciences, 42. DOI: https://doi.org/10.4025/actascibiolsci.v42i1.52473
Dildar, T., Cui, W., Ikhwanuddin, M., & Ma, H. (2025). Aquatic organisms in response to salinity stress: Ecological impacts, adaptive mechanisms, and resilience strategies. Biology, 14(6). DOI: https://doi.org/10.3390/biology14060667
Effendie, M. I. (2002). Biologi perikanan. Bogor: Yayasan Pustaka Nusantara.
El Rahimi, S. A., Razeky, M. M., Perdana, A. W., & Putra, D. F. (2021). The growth performance of common carp (Cyprinus carpio) co cultured with different vegetable plants in aquaponics system. Depik, 10(1): 30–34. DOI: https://doi.org/10.13170/depik.10.1.19467
El Saadony, M. T., Alagawany, M., Patra, A. K., Kar, I., Tiwari, R., Dawood, M. A. O., Dhama, K., & Abdel Latif, H. M. R. (2021). The functionality of probiotics in aquaculture: An overview. Fish and Shellfish Immunology, 117. DOI: https://doi.org/10.1016/j.fsi.2021.07.007
Elsabagh, M., Mohamed, R., Moustafa, E. M., Hamza, A., Farrag, F., Decamp, O., Dawood, M. A. O., & Eltholth, M. (2018). Assessing the impact of Bacillus strains mixture probiotic on water quality, growth performance, blood profile and intestinal morphology of Nile tilapia (Oreochromis niloticus). Aquaculture Nutrition, 24(6). DOI: https://doi.org/10.1111/anu.12797
Fahira, R., Putra, D. F., Wahab, G. A., Ismarica, & Irwan. (2025). Combination of moringa leaf meal (Moringa oleifera) and black soldier fly larvae meal (Hermetia illucens) in feed to support growth performance in striped catfish (Pangasius sp.). Journal of Vocational in Aquaculture (JAVA), 1(2). DOI: https://doi.org/10.29244/java.v1i2.61885
Fioramonti, J., Theodorou, V., & Bueno, L. (2003). Probiotics: What are they? What are their effects on gut physiology? Best Practice & Research Clinical Gastroenterology, 17(5): 711–724. DOI: https://doi.org/10.1016/S1521-6918(03)00075-1
Furuya, W. M., Cruz, T. P., & Gatlin, D. M. (2023). Amino acid requirements for Nile tilapia: An update. Animals, 13(5). DOI: https://doi.org/10.3390/ani13050900
Ghori, I., Tubassam, M., Ahmad, T., Zuberi, A., & Imran, M. (2022). Gut microbiome modulation mediated by probiotics: Positive impact on growth and health status of Labeo rohita. Frontiers in Physiology, 13. DOI: https://doi.org/10.3389/fphys.2022.949559
Gunawan, G., Huzy, O. M., Jalil, Z., & Putra, D. F. (2024). The effect of frangipani leaf extract (Plumeria sp.) on the hatchability and growth of sangkuriang catfish (Clarias gariepinus) larvae. BIO Web of Conferences, 87: 03015. DOI: https://doi.org/10.1051/bioconf/20248703015
Hassan, M. A., Fathallah, M. A., Elzoghby, M. A., Salem, M. G., & Helmy, M. S. (2022). Influence of probiotics on water quality in intensified Litopenaeus vannamei ponds under minimum water exchange. AMB Express, 12(1). DOI: https://doi.org/10.1186/s13568-022-01370-5
Howe, J. C. (1996). Fish nutrition in aquaculture. Fisheries Research, 26(3–4). DOI: https://doi.org/10.1016/S0165-7836(96)90006-5
Ismarica, I., Putra, D. F., & Khairina, U. (2022). Ontogeny and morphological development of tilapia (Oreochromis niloticus) larvae in Aceh Province, Indonesia. E3S Web of Conferences, 339: 01007. DOI: https://doi.org/10.1051/e3sconf/202233901007
Iyer, R., Tomar, S. K., Uma Maheswari, T., & Singh, R. (2010). Streptococcus thermophilus strains: Multifunctional lactic acid bacteria. International Dairy Journal, 20(3). DOI: https://doi.org/10.1016/j.idairyj.2009.10.005
Juanda, I. R., Putra, D. F., & Arisa, I. I. (2024). Pengaruh penambahan fermentasi jahe (Zingiber officinale) dalam pakan untuk meningkatkan pertumbuhan dan kelangsungan hidup ikan nila (Oreochromis niloticus). Jurnal Kelautan dan Perikanan Indonesia, 4(1): 19–28. DOI: https://doi.org/10.24815/jkpi.v4i1.29733
Karpunina, L. V., Poddubnaya, I. V., Vasiliev, A. A., Uryadova, G. T., & Rysmukhambetova, G. E. (2022). Influence of the exopolysaccharide Streptococcus thermophilus on the development of the Lena sturgeon. The Agrarian Scientific Journal, 12: 62–65. DOI: https://doi.org/10.28983/asj.y2022i12pp62-65
Leong, S. S., Korel, F., Lingoh, A. D., Sarbini, S. R., Toh, S. C., Abit, L. Y., & Wong, S. C. (2023). Current probiotics application for aquaculture feed: A review. Borneo Science, 44(2). DOI: https://doi.org/10.51200/bsj.v44i2.4703
Muhammadar, A. A., Chaliluddin, M. A., Putra, D. F., & Asmawati, M. S. (2018). Study of probiotics of yeast and lactic acid bacteria in feeding on culture of larvae shrimp (Penaeus monodon). IOP Conference Series: Earth and Environmental Science, 216(1): 012031. DOI: https://doi.org/10.1088/1755-1315/216/1/012031
Muhammadar, A. A., Malasari, N., Afriani, S, Asmawati, M. S., & Putra, D. F. (2023). Effect of addition of rubber seeds flour (Hevea brasiliensis) in fish feed on growth and feeding efficiency of gourami (Osphronemus goramy). IOP Conference Series: Earth and Environmental Science, 1221(1): 012049. DOI: https://doi.org/10.1088/1755-1315/1221/1/012049
Mujeeb, I., Ali, S. H., Qambrani, M., & Ali, S. A. (2022). Marine bacteria as potential probiotics in aquaculture. Journal of Microbiology, Biotechnology and Food Sciences, 12(2). DOI: https://doi.org/10.55251/jmbfs.5631
Mustafa, A., Syah, R., Paena, M., Sugama, K., Kontara, E. K., Muliawan, I., Suwoyo, H. S., Asaad, A. I. J., Asaf, R., Ratnawati, E., Athirah, A., Makmur, Suwardi, & Taukhid. (2023). Strategy for developing whiteleg shrimp (Litopenaeus vannamei) culture using intensive/super intensive technology in Indonesia. Sustainability, 15(3). DOI: https://doi.org/10.3390/su15031753
Putra, D. F. (2021). Dasar dasar budidaya perairan. Banda Aceh: Syiah Kuala University Press.
Putra, D. F., Abbas, M. A., Solin, N., & Othman, N. (2022). Effects of dietary Caulerpa lentillifera supplementation on growth performance and survival rate of milkfish (Chanos chanos). Elkawnie, 7(2): 395. DOI: https://doi.org/10.22373/ekw.v7i2.10484
Putra, D. F., Astari, B., & Arisa, I. I. (2025). Indigofera sp. leaf flour stimulate milkfish growth performance (Chanos chanos). BIO Web of Conferences, 156: 03040. DOI: https://doi.org/10.1051/bioconf/202515603040
Putra, D. F., Muhsinah, M., & Arisa, I. I. (2021). The substitution of soybean meal by fermented tofu dregs in the milkfish (Chanos chanos) diet. IOP Conference Series: Earth and Environmental Science, 674(1): 012102. DOI: https://doi.org/10.1088/1755-1315/674/1/012102
Qiu, Z., Xu, Q., Li, S., Zheng, D., Zhang, R., Zhao, J., & Wang, T. (2023). Effects of probiotics on the water quality, growth performance, immunity, digestion, and intestinal flora of giant freshwater prawn (Macrobrachium rosenbergii) in the biofloc culture system. Water, 15(6). DOI: https://doi.org/10.3390/w15061211
Ren, W., Wu, H., Guo, C., Xue, B., Long, H., Zhang, X., Cai, X., Huang, A., & Xie, Z. (2021). Multi strain tropical Bacillus spp. as a potential probiotic biocontrol agent for large scale enhancement of mariculture water quality. Frontiers in Microbiology, 12. DOI: https://doi.org/10.3389/fmicb.2021.699378
Safriani, I., Putra, D. F., Rahimi, S. A. E., & Othman, N. (2019). Black tiger shrimp larvae (Penaeus monodon) that received eggshell powder in diet exhibit decreasing of growth and survival rate. IOP Conference Series: Earth and Environmental Science, 348(1): 012098. DOI: https://doi.org/10.1088/1755-1315/348/1/012098
Sunitha, K., & Krishna, P. V. (2016). Efficacy of probiotics in water quality and bacterial biochemical characterisation of fish ponds. International Journal of Current Microbiology and Applied Sciences, 5(9). DOI: https://doi.org/10.20546/ijcmas.2016.509.004
Tabassum, T., Mahamud, A. G. M. S. U., Acharjee, T. K., Hassan, R., Snigdha, T. A., Islam, T., Alam, R., Khoiam, M. U., Akter, F., Azad, M. R., Al Mahamud, M. A., Ahmed, G. U., & Rahman, T. (2021). Probiotic supplementations improve growth, water quality, hematology, gut microbiota and intestinal morphology of Nile tilapia. Aquaculture Reports, 21: 100972. DOI: https://doi.org/10.1016/j.aqrep.2021.100972
Tan, S. Y., Sethupathi, S., Leong, K. H., & Ahmad, T. (2024). Challenges and opportunities in sustaining aquaculture industry in Malaysia. Aquaculture International, 32(1). DOI: https://doi.org/10.1007/s10499-023-01173-w
Vasyliuk, O. M., Skrotskyi, S. O., Khomenko, L. A., & Babich, T. V. (2023). Probiotics based on lactic acid bacteria for aquaculture. Mikrobiolohichnyi Zhurnal, 85(2). DOI: https://doi.org/10.15407/microbiolj85.02.075
Villarreal, H. (2023). Shrimp farming advances, challenges, and opportunities. Journal of the World Aquaculture Society, 54(5). DOI: https://doi.org/10.1111/jwas.13027
Yossa, R., Fatan, N. A., & Palanivelu, K. (2023). Re evaluating the dietary methionine requirements in an improved strain of Nile tilapia (Oreochromis niloticus). Heliyon, 9(3): e14376. DOI: https://doi.org/10.1016/j.heliyon.2023.e14376
Yue, G. H., Ma, K. Y., & Xia, J. H. (2024). Status of conventional and molecular breeding of salinity tolerant tilapia. Reviews in Aquaculture, 16(1). DOI: https://doi.org/10.1111/raq.12838
Yuvaraj, D., & Karthik, R. (2015). Efficacy of probiotics on Litopenaeus vannamei culture through zero water exchange system. Journal of Fisheries and Aquatic Science, 10(6): 445–463. DOI: https://doi.org/10.3923/jfas.2015.445.463
Zhao, C., Guo, G., Li, Z., Chen, J., & Ren, Y. (2024). Effects of probiotics (Bacillus coagulans) supplementation after antibiotic administration on growth, immunity, and intestinal microflora in turbot (Scophthalmus maximus). Aquaculture International, 32(2). DOI: https://doi.org/10.1007/s10499-023-01225-1
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