Treatment of PLGA Nanoparticles Ointment-Ethanol Extract of Archidendron pauciflorum in the Wound Healing in Diabetic Mice


Desak Made Malini(1*), Yasmi Purnamasari Kuntana(2), Madihah Madihah(3), Wildanul Furqon(4), Wawan Hermawan(5)

(1) Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia. Tel./Fax. +62-22-7796412, Indonesia
(2) Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia. Tel./Fax. +62-22-7796412, Indonesia
(3) Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia. Tel./Fax. +62-22-7796412, Indonesia
(4) Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia. Tel./Fax. +62-22-7796412, Indonesia
(5) Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Bandung-Sumedang Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia. Tel./Fax. +62-22-7796412, Indonesia
(*) Corresponding Author

Abstract


Diabetic wounds lead to severe tissue damage and are diffi-cult to cure. Jengkol (Archidendron pauciflorum) is a plant commonly used by local Indonesian communities to treat diabetic wounds. The efficiency of herbal medicine still has a deficiency of its ability to reach the target organs, therefore nanotechnology is applied in the hope that all drug concentrations can reach the target organs successfully. This study aimed to evaluate the effectiveness of PLGA nanoparticle ointment-ethanol extract jengkol fruit peel (EEJFP) to accelerate the wound healing process in the skin of streptozotocin-induced diabetic mice. The research method used was experimental with a completely randomized design using six treatments and four replications. Diabe-tes was induced by intraperitoneal injection of streptozotocin 180 mg/ kg BW. Mice with a blood glucose level of ≥150 mg/dL were used for diabetic mice models. The incision wound created at the dorsolateral region of shaven skin at ±1 cm2 using sterile scissors. The treatments given were vaseline for Control Negative (CN) and Control Positive (CP), Betadine ointment (PB), 10% EEJFP ointment (P1), 5% PLGA nanoparticle ointment-EEJFP (P2), and 2.5% PLGA nanoparticle ointment-EEJFP (P3). The results showed that the administration of PLGA nanoparticles ointment-EEJFP with a concentration of 5% PLGA nanoparticle ointment-EEJFP (P2) resulted in the shortest wound length on day 3, 7 and 14; narrower granulation tissue; a larger number of blood capillaries; and denser collagen fibers (α <0.05) compared to CP and PB treatments. The administration of PLGA nanoparticle ointment-EEJFP with a concentration of 5% was the most effective concentration in accelerating wound healing in the skin of diabetic mice. 


Keywords


: ointment, nanoparticles, PLGA, Jengkol, diabetic wounds.

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References


Agarwal, P. K., Singh, A., Gaurav, K., Goel, S., Khanna, H. D. & Goel, R. K. (2009). Evaluation of Wound Healing Activ-ity of Extracts of Plantain Banana (M.

sapientum var. paradisiaca) in rats. In-dian Journal of Experimental Biology, 47(1), 322-40.

Aloys, H., Korma, S. A., Alice, T. M., Chan-tal, N., Ali, A. H., Abed, S. M. & Lide-phonse, H. (2016). Microencapsulation by Complex Coacervation: Methods, Techniques , Benefits , and Applications - A Review. American Journal of Food Science and Nutrition Research, 3(6), 188–192.

Chen, J., Kamper, M., Heck, T., Nakagawa, K., Humpert, P. M., Bai, L., Wu, G., Zhang, Y., Luther, T., Andrassay, M., Shicekofer, S., Hamann, A., Morcos, M., Chain, B., Ster, M. D., Naworth, P. & Bierhaus, A. (2005). Tissue Factor as a Link Between Wounding and Tissue Repair. Diabetes, 52(2), 2143-2154.

Chereddy, K. K., Her, C., Comune, M., Moia, C., Lopes, A., Porporato, P. E., Vanack-er, J., Lam, M. C., Steinstraesser, L., Sonveaux, P., Zhu, H., Ferreira, L. S., Vandermeulen, G. & Préat, V. (2014). PLGA Nanoparticles Loaded with Host Defense Peptide LL37 Promote Wound Healing. Journal of Controlled Release, 8(16), 1-12.

Danhier, F., Ansorena, E., Silva, J. M., Coco, R., Le, B. A. & Préat, V. (2012). PL-GA-based Nanoparticles: An Overview of Biomedical Applications, Journal of Controlled Release, 161(2), 505-522.

Fessi, H., Puisieux, F., Devissaguet, J. P., Am-moury, N. & Benita, S. (1999). Nano-capsule Formation by Interfacial Polymer Deposition Following Solvent Dis-placement. International Journal of Pharmaeutical, 55(1), 1-4.

Francis, G., Zohar, K., Harinder, P. S., Makkar & Klaus, B. (2002). The Biological Action of Saponins in Animal Systems: a Review. The British of Journal, 88(6), 587-605.

Furman, B. L. (2015). Streptozotocin-induced Diabetic Models in Mice and Rats. Cur-rent Protocols in Pharmacology, 70(5), 1-20.

Gurtner, G. C. (2007). Wound Healing: Nor-mal and Abnormal. Philadelphia: Grabb dan Smith’s Plastic Surgey Sixth edi-tion.

Hartanto, T. (2013). Pengaruh Antimikroba Ekstrak Kulit Manggis (Garcinia mangostana L.) terhadap Pertumbuhan Shigella dysenteriae secara in vitro. Semarang: Fakultas Kedokteran Uni-versitas Islam Sultan Agung.

Keller, U., Kumin, A., Braun, S. & Werner, S. (2006). Reactive Oxygen Species and their Detoxification in Healing Skin Wound. Journal of Investigative Dermatology Symposium Proceeding, 11(1), 106-111.

Khan, Z. I., Badrun, N., Abu, J., Shahnaz, R., Majeedul, H. C. & Mohammed, R. (2010). An Evaluation of Antihyper-glicemic and Antinociceptive Effects of Methanol Extract of Cassia fistula (Fabaceae) Leaves in Swiss Albino Mice. Advances in Natural and Applied Sciences, 4(3), 305-310.

Malini, D. M., Madihah., Joko, K., Fitri, K. & Johan, I. (2017). Ethnobotanical Study of Medicinal Plants in Karangwangi, District of Cianjur, West Java. Biosain-tifika, 9(2), 345-356.

Mathur, M. & Govind, V. (2013). Role of Nanoparticles for Production of Smart Herbal Drug. Indian Journal of Natural Products and Resources, 4(4), 329-338.

Napangala, A., Susianti & Apriliana, E. (2012). Pengaruh Pemberian Getah Tanaman Jarak Pagar (Jatropha curcas L.) Secara Topikal terhadap Tingkat Ke-sembuhan Luka Iris pada Tikus Jantan Putih Galur Spargue Dawley. Medical Journal of Lampung University, 3(5), 26-35.

Nisa, M. V., Meilawaty, Z. & Astuti, P. (2013). Efek Pemberian Ekstrak Daun Singkong (Manihot esculenta) terhadap Proses Penyembuhan Luka Gingiva Tikus (Rattus norvegicus). Thesis. Jember: Fakultas Kedokteran Gigi Universitas Jember.

Raffa, V., Vittorio, O., Riggio, C. & Cuschieri, A. (2010). Progress in Nanotechnology for Healthcare. Minim Invasive Ther Al-lied Technology, 19(3), 127-35.

Rahmawati, I. (2014). Perbedaan Efek Perawatan Luka Menggunakan Geru-san Daun Petai Cina (Leucaena glauca Benth) dan Povidon Iodine 10% dalam Mempercepat Penyembu-han Luka Bersih pada Marmut (Cavia porcellus), 1(2), 227-234.

Robert, G. F., Zgonis, T., Armstrong, D. G., Driver, V. R., Giurini, J. M., Kravitz, S. R., Landsman, A. S., Lavery, L. A., Moore, J. C., Schuberth, J. M., Wukich, D. K., Andersen, C. & Vanore, J. V. (2006). Diabetic Foot Disorder a Clini-cal Practice Guideline. USA: American College of Foot and Ankle Surgeons.

Sahoo, S. K. & Labhasetwar, V. (2003). Nano-tech Approaches to Drug Delivery and Imaging. Drug Discov Today, 8(11), 12- 20.

Sari, F. P. & Sari, S. M. (2011). Ekstraksi Zat Aktif Antimikroba dari Tanaman Yodi-um (Jatropha multifida Linn) sebagai Bahan Baku Alternatif Antibiotik alami. Thesis. Semarang: Fakultas Teknik Uni-versitas Diponegoro.

Saroja, M., Santhi, R. & Annapoorani, S. (2012). Wound Healing Activity of Fla-vonoid Fraction of Cynodon Dactylon in Swiss Albino Mice. International Research Journal of Pharmacy, 3(2), 23-31.

Sudayo, A. W., Setiyohadi, B., Alwi, I., Si-madibrata, M. K. & Setiati, S. (2009). Buku Ajar Ilmu Penyakit Dalam Edisi V Jilid III. Jakarta: Internal Publishing.

Wientarsih, I., Winarsih, W. & Sutardi, I.N. (2012). Aktivitas Penyembuhan Luka oleh Gel Fraksi Etil Asetat Rimpang Kunyit pada Mencit Hiperglikemik. Jur-

nal Veteriner, 13(3), 251-256.

Winarsih, W., Wientarsih, I. & Sutardi, I. N. (2012). Aktivitas Salep Ekstrak Rim-pang Kunyit dalam Proses Penyembu-han Luka pada Mencit yang Diinduksi Diabetes. Jurnal Veteriner, 13(3), 242- 250.

Yanhendri & Yenny, S. W. (2012). Berbagai Bentuk Sediaan Topikal dalam Der-matologi. Cermin Dunia Kedokteran, 39(6), 423-430.




DOI: https://doi.org/10.15575/biodjati.v5i2.9256

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