Formula padat Bacillus cereus STRAIN TLE1.1 untuk pengendalian penyakit busuk pangkal batang (Sclerotium rolfsii) pada tanaman tomat

Authors

  • Yulmira Yanti andalas university padang, Indonesia
  • Hasmiandy Hamid
  • Reflin Reflin
  • Yaherwandi Yaherwandi
  • Febri Yani Chrismont

DOI:

https://doi.org/10.15575/14679

Keywords:

busuk pangkal batang, formula padat, Sclerotium rolfsii, tomat

Abstract

Penyakit utama tanaman tomat yaitu busuk pangkal batang yang disebabkan oleh Sclerotium rolfsii dapat menimbulkan kerugian mencapai 80-100%. Tujuan penelitian yaitu untuk mendapatkan formula padat Bacillus cereus strain TLE1.1 yang efektif untuk pengendalian penyakit busuk pangkal batang pada tanaman tomat. Penelitian bersifat eksperimen dengan mengamati kemampuan formula padat B.cereus strain TLE1.1 dalam pengendalian penyakit busuk pangkal batang dengan Rancangan Acak Lengkap yang terdiri atas 9 perlakuan dan 3 ulangan. Perlakuan terdiri atas kombinasi bahan pembawa formula padat yang terdiri atas limbah padat ampas tebu, ampas tahu dan tongkol jagung, fungisida serta kontrol. Masing-masing formula padat B. cereus strain TLE1.1 diintroduksi pada benih dan bibit tomat. Hasil penelitian menunjukkan bahwa hampir semua formula mampu menekan penyakit busuk pangkal batang tanaman tomat. Formula terbaik dalam menurunkan penyakit busuk pangkal batang pada tanaman yaitu formula ampas tahu dan ampas tahu + tongkol jagung.

 

Main disease of tomato plant, namely stem rot caused by Sclerotium rolfsii which can cause losses up to 80-100%. The aim of the study was to obtain a solid formula of Bacillus cereus strain TLE1.1 which was effective for controlling stem rot disease in tomato plant. This research was an experimental study to know the ability of the solid formula of B. cereus strain TLE1.1 in controlling stem rot disease which was carried out in a completely randomized design consisting of 9 treatments and 3 replications. The treatment consisted of a combination of solid formula carriers consisting of sugarcane solid waste, tofu dreg and corncob, fungicides and controls. Each solid formula of B. cereus strain TLE1.1 was introduced into tomato seeds and seedlings. The results showed that almost all of the formulas were able to suppress stem base disease of tomato plants. The best formula that reduced stem rot in plants were the tofu dreg and tofu dreg + corncob formula.

Author Biography

Yulmira Yanti, andalas university padang

Plant protection

References

Abidin, Z., Aini, L. Q., & Abadi, A. L. (2015). Pengaruh bakteri Bacillus sp. dan Pseudomonas sp. terhadap pertumbuhan jamur patogen Sclerotium rolfsii Sacc. penyebab penyakit rebah semai pada tanaman kedelai. Jurnal Hama dan Penyakit Tumbuhan, 3(1), 1–10. http://jurnalhpt.ub.ac.id/index.php/jhpt/article/view/158

Diarta, I. M., Javandira, C., & Widnyana, I. K. (2016). Antagonistik Bakteri Pseudomonas Spp. Dan Bacillus Spp. Terhadap Jamur Fusarium Oxysporum Penyebab Penyakit Layu Tanaman. Jurnal Bakti Saraswati, 5(1), 70–76. https://media.neliti.com/media/publications/74991-ID-none.pdf

Dixit, R., Agrawal, L., Gupta, S., Kumar, M., Yadav, S., Chauhan, P., & Nautiyal, C. (2016). Southern blight disease of tomato control by 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing Paenibacillus lentimorbus B-30488. Plant Signaling & Behavior, 11, 0. https://doi.org/10.1080/15592324.2015.1113363

Flori, F., Mukarlina, & Rahmawati. (2020). Potensi antagonis isolat bakteri bacillus spp. asal rizosfer tanaman lada (Piper nigrumL.) sebagai agen pengendali jamur fusarium sp.JDF. Bioma, 5(1), 111–120. https://doi.org/https://doi.org/10.20956/bioma.v5i1.9923

Joo, G. J., Kim, Y. M., Lee, I. J., Song, K. S., & Rhee, I. K. (2004). Growth promotion of red pepper plug seedlings and the production of gibberellins by Bacillus cereus, Bacillus macroides and Bacillus pumilus. Biotechnology Letters, 26(6), 487–491. https://doi.org/https://doi.org/10.1023/B:BILE.0000019555.87121.34

Kator, L., Yula Hosea, Z., & Daniel Oche, O. (2015). Sclerotium rolfsii; Causative organism of southern blight, stem rot, white mold and sclerotia rot disease. Scholars Research Library Annals of Biological Research, 6(11), 78–89. http://scholarsresearchlibrary.com/archive.html

Klement, Z., Rudolph, K., & Sand, D. . (1990). Methods in Phytophatology. y. Hungary:

Kwon, & Park, T. (2002). Pictorial atlas of soil and seed fungi morphologies of cultured fungi and key to species (Second edi). CRC Press Boca Raton.

Natalia, A. G., Aeny, T. N., & Prasetyo, J. (2014). Uji keefektitasan Trichoderma spp. dengan bahan campuran yang berbeda dalam menghambat pertumbuhan Sclerotium rolfsii penyebab penyakit rebah kecambah pada kacang tanah. Jurnal Agrotek Tropika, 2(3), 408–413. https://doi.org/10.23960/jat.v2i3.2070

Oktania, P., Marwan, H., & Asniwita, A. (2018). Potensi Bacillus spp. dari rizosfer tanaman kedelai untuk mengendalikan penyakit rebah kecambah (Sclerotium rolfsii Sacc.). Jurnal Agroecotania : Publikasi Nasional Ilmu Budidaya Pertanian, 1(1), 19–32. https://doi.org/10.22437/agroecotania.v1i1.5333

Oktrisna, D., Puspita, F., & Zuhry, E. (2017). Uji bakteri Bacillus Sp. endofit diformulasi dengan beberapa limbah terhadap tanaman padi sawah (Oryza Sativa L.). Riau University.

Rivard, C. L., O’connell, S., Peet, M. M., Welker, R. M., & Louws, F. J. (2012). Grafting tomato to manage bacterial wilt caused by Ralstonia solanacearum in the southeastern United States. Plant disease, 96(7), 973–978.

Saeed, S., Butt, B. Z., Sana, N., & Javaid, A. (2016). Biological control of Sclerotium rolfsii through the leaf extract of Melia azedarach L. and Syzigium cumini. J. Med. Plant, 4(5), 259–261.

Sahu, P. K., Brahmaprakash, G. P., Gupta, A., Saxena, A. K., Singh, S., & Singh, U. B. (2019). Antagonistic potential of bacterial endophytes and induction of systemic resistance against collar rot pathogen Sclerotium rolfsii in tomato. Biological Control : Theory and Applications in Pest Management, 137, Not Available. https://doi.org/10.1016/j.biocontrol.2019.104014

Schaad, N. ., Jones, J. ., & Chun, W. (2001). Laboratory Guide for Identification of Plant Pathogenic Bacteria (3rd изд). APS Press.

Sekhar, J. C., Mishra, J. P., Prasad, R., Reddy, V. P., Kumar, S., Thakur, A., & Pal, J. (2020). Isolation and in vitro evaluation of biocontrol agents, fungicides and essential oils against stem blight of tomato caused by Sclerotium rolfsii (Curzi) CC Tu & Kimber. J Pharmacogn Phytochem, 9(3), 700–705. https://www.phytojournal.com/archives/2020/vol9issue3/PartK/9-3-28-316.pdf

Taghavi, S., Barac, T., Greenberg, B., Borremans, B., Vangronsveld, J., & van der Lelie, D. (2005). Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene. Applied and Environmental Microbiology, 71(12), 8500–8505. https://doi.org/10.1128/aem.71.12.8500-8505.2005

Wang, S.-S., Liu, J.-M., Sun, J., Sun, Y.-F., Liu, J.-N., Jia, N., Fan, B., & Dai, X.-F. (2019). Diversity of culture-independent bacteria and antimicrobial activity of culturable endophytic bacteria isolated from different Dendrobium stems OPEN. Scientific Reports, 9(10389), 1–12. https://doi.org/10.1038/s41598-019-46863-9

Watanabe, T. (2002). Pictorial atlas of soil and seed fungi morphologies of cultured fungi and key to species (Second). CRC Press Boca Raton London Newyork.

Wijayanti, E., & Susila, A. D. (2013). Pertumbuhan dan produksi dua varietas tomat (Lycopersicon esculentum Mill.). Bul. Agrohorti, 1(1), 104–112. https://doi.org/https://doi.org/10.29244/agrob.1.1.104-112

Yanti Yulmira, Warnita, W., Reflin Reflin, & Busniah Muzir. (2017). Identification and characterizations of potential indigenous endophytic bacteria which had ability to promote growth rate of tomato and biocontrol agents of Ralstonia solanacearum and Fusarium oxysporum fsp. solani. Microbiology Indonesia, 11(4), 117–122. https://doi.org/10.5454/mi.11.4.2

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Published

2022-01-01

Issue

Vol. 8 No. 2 (2021)

Section

Original Research Articles

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