Rhizoctonia mycorrhizae application and watering intervals on Dendrobium violaceoflavens seedling: a study of its effect on drought stress


R Soelistijono(1*), Daryanti Daryanti(2), Haryuni Haryuni(3), Irvansyah Cahya Perwita(4), Dian Rakhmawati(5)

(1) Scopus ID: 57194008516, Universitas Tunas Pembangunan, Indonesia
(2) Tunas Pembangunan University,  
(3) Tunas Pembangunan University,  
(4) Tunas Pembanguna University,  
(5) Magelang District’s Government,  
(*) Corresponding Author

Abstract


Dendrobium violaceoflavens merupakan spesies asli Papua, habitat alaminya adalah daerah dengan curah hujan tinggi sehingga akan menjadi masalah bila dibudidayakan dan terlambat dalam penyiraman. Penelitian bertujuan mengetahui pengaruh aplikasi Rhizoctonia mikoriza dan interval penyiraman pada pertumbuhan vegetatif bibit D. violaceoflavens terhadap cekaman kekeringan (faktor abiotik). Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 2 faktor dan 5 ulangan. Faktor pertama: dengan dan tanpa aplikasi Rhizoctonia mikoriza, faktor kedua adalah interval penyiraman 2 hari, 4 hari dan 6 hari. Hasil penelitian menunjukkan [1] aplikasi Rhizoctonia mikoriza berpengaruh nyata terhadap tinggi tanaman dengan nilai tertinggi 2,43 cm, jumlah daun 3,17 helai, dan jumlah akar 4,6 helai; [2] Interval penyiraman berpengaruh nyata terhadap tinggi tanaman dengan nilai tertinggi 2, 85 cm, jumlah daun 3,35 helai, jumlah akar 5,27 helai, dan bobot segar 1,23 g; [3] Interaksi pemberian Rhizoctonia mikoriza dan interval penyiraman 4 hari berpengaruh nyata pada panjang daun 1,90 cm dan jumlah daun 3,70 helai, dan pada akar terbentuk struktur peloton. Hasil terbaik diperoleh pada aplikasi Rhizoctonia mikoriza dan interval penyiraman 4 hari dalam penanggulangan cekaman kekeringan.

ABSTRACT

Dendrobium violaceoflavens is a native species of Papua, its natural habitat is an area with high rainfall that will be a problem if it is cultivated and watering is delayed. The aim of this study was to determine the effect of Rhizoctonia mycorrhizae application and watering interval on vegetative growth of D. violaceoflavensseedlings on drought stress (abiotic factors).Research used a Completely Randomized Design (CRD) with 2 factors and 5 replications. First factor was with and without application of Rhizoctonia mycorrhizae, second factor was watering interval of 2 days, 4 days and 6 days. The result showed that [1] the application of Rhizoctonia mycorrhizae had a significant effect on plant height with the highest value of 2.43 cm, number of leaves 3.17 leaves, number of roots 4.6 leaves; [2] watering interval significantly affected on plant height with the highest value of 2.85 cm, number of leaves 3.35 sheets , number of roots 5.27, fresh weight of 1.23 g; [3] Interaction between Rhizoctonia mycorrhizae and watering interval of 4 days significantly affected on leaf length 1.90 cm, leaf number 3.70 sheets and a peloton structure was formed on the root. Best results were obtained on the application of Rhizoctonia mycorrhizae and watering interval of 4 days in overcoming the drought stress.


Keywords


D. violaceoflavens, Rhizoctonia mycorrhizae, Watering Intervals, Water stress

Full Text:

PDF

References


Agrios, G. N. (2004). Plant Pathology. 4th ed. Academic press. New York.

Balestrini, R., Nerva, L., Sillo, F., Girlanda, M., & Perotto, S. (2014). Plant and fungal gene expression in mycorrhizal protocorms of the orchid Serapias vomeracea colonized by Tulasnella calospora. Plant Signaling & Behavior, 9(11), e977707.

Cui, Y.-Y., Pandey, D. M., Hahn, E.-J., & Paek, K.-Y. (2004). Effect of drought on physiological aspects of Crassulacean acid metabolism in Doritaenopsis. Plant Science, 167(6), 1219–1226. https://doi.org/https://doi.org/10.1016/j.plantsci.2004.06.011

Dwiyanto, W., Soelistijono, R., & Utami, D. S. (2017). Rhizoctonia mikoriza anggrek vanda limbata characterization isolate the morphology and anatomy of orchid mycorrhizal Rhizoctonia Vanda limbata. Agreneca, 17(I), 1–11.

George, E. F., Hall, M. A., & De Klerk, G.-J. (2008). Plant propagation by tissue culture 3rd Edition. Micropropagation: Uses and Methods, 1, 29–64.

Ginting, B., Prasetio, W., & Sutater, T. (2001). Pengaruh cara pemberian air, media, dan pemupukan terhadap pertumbuhan anggrek Dendrobium. J. Hort, 11(1), 22–29.

Hatni, F. (2017). Karakterisasi Planlet Anggrek Bulan (Phalaenopsis amabilis (L.) Bl.) Hasil Inokulasi Rhizoctonia sp. dan Induksi Asam Salisilat Secara in Vitro. UNIVERSITAS LAMPUNG.

Herliana, O., Rokhminarsi, E., Mardini, S., & Jannah, M. (2018). Pengaruh jenis media tanam dan aplikasi pupuk hayati mikoriza terhadap pertumbuhan, pembungaan dan infeksi mikoriza pada tanaman anggrek Dendrobium sp. Kultivasi, 17(1), 550–557. https://doi.org/10.24198/kultivasi.v17i1.15774

Kasiamdari, R. S. (2000). Binucleate Rhizoctonia isolate from mycorrhizal pot cultures: its morphological characteristics and pathogenicity. Berkala Ilmiah Biologi, 2(2000).

Munir, R., & Zulman, H. U. (2011). Pengaruh berbagai media dengan inokulan mikoriza terhadap aklimatisasi anggrek Dendrobium (Dendrobium sp.). Jerami.

Ningsih, R., & Febrianti, D. (2014). Identifikasi mikoriza anggrek Spathoglottis plicata Blume. dan Phalaenopsis amabilis L. Identification of orchid mycorrhiza of Spathoglottis plicata Blume. and Phalaenopsis amabilis L. BioWallacea: Jurnal Penelitian Biologi (Journal of Biological Research), 1(1).

Nurcahyani, E., & Sabatini, A. P. (2022). Respon planlet anggrek Cattleya sp. hasil seleksi in vitro terhadap cekaman kekeringan dengan Poli etilen glikol (PEG) 6000. Indonesian Journal of Biotechnology and Biodiversity, 6(2), 61–67.

Nusantara, A. D. (2007). Baku mutu inokulum cendawan mikoriza arbuskula. Makalah Workshop Mikoriza. Kongres Nasional Mikoriza Indonesia II. Asosiasi Mikoriza Indonesia. Bogor.

Pietro. (2002). Monaco Natura encyclopedia discover the biodiversity (Dendrobium violaceoflavens).

SARI, D. N. (2018). Identifikasi Orchid Mycorrhiza Pada Akar Anggrek Cattleya Sp dan Dendrobium Sp. UIN Raden Intan Lampung.

Senthilkumar, S., Saravanakumar, P., Krishnamurthy, K. V, & Britto, J. (2001). Morphological and structural features of mycorrhizal roots of Spathoglottis plicata and Dendrobium species. Phyta, 5(1), 24–31.

Siddiqui, Z. A., & Pichtel, J. (2008). Mycorrhizae: an overview. In Mycorrhizae: Sustainable Agriculture and Forestry (pp. 1–35). Springer.

Sneh, B., Yamoah, E., & Stewart, A. (2004). Hypovirulent Rhizoctonia spp isolates from New Zealand soils protect radish seedlings against dampingoff caused by R. solani. New Zealand Plant Protection, 57, 54–58.

Soelistijono, R., Utami, D. S., Daryanti, Faizin, M., & Dian, R. (2020). Short communication: Characterization of Rhizoctonia -like mycorrhizae associated with five Dendrobium species in Java, Indonesia. Biodiversitas, 21(3), 1007–1011. https://doi.org/10.13057/biodiv/d210321

Sukma, K. P. W. (2015). Mekanisme tumbuhan menghadapi kekeringan. Wacana Didaktika, 3(2), 186–194.

Zimmer, K., Hynson, N. A., Gebauer, G., Allen, E. B., Allen, M. F., & Read, D. J. (2007). Wide geographical and ecological distribution of nitrogen and carbon gains from fungi in pyroloids and monotropoids (Ericaceae) and in orchids. New Phytologist, 175(1), 166–175.




DOI: https://doi.org/10.15575/21123

Refbacks

  • There are currently no refbacks.


Creative Commons Licence

Jurnal Agro (J. Agro: ISSN 2407-7933) by http://journal.uinsgd.ac.id/index.php/ja/index is licensed under a Creative Commons Attribution 4.0 International License.