Karakteristik fisik carbon dots dan aplikasinya dengan pemupukan untuk meningkatkan produksi dan mutu benih padi
Physical characteristics of carbon dots and its application with fertilizer to increase rice seed production and quality
DOI:
https://doi.org/10.15575/j.agro.46880Abstract
Carbon dots (CDs) are nano-sized carbon particles proven to enhance seed germination, plant growth, and yield. This study evaluated CDs in an integrated fertilization system combining macronutrient fertilizers (NPK) and organic matter (OM). The experiment was conducted during the 2023 dry season at the Sukamandi Experimental Station of BBRMP Padi using a Split-split Plot Design. The treatments included NPK combinations (main plot; without NPK, +PK, +NP, +NK, +NPK), OM (sub-plots; manure, straw compost, without OM), and foliar-applied CDs (800 mg L⁻¹) (sub-sub-plots; +CDs, without CDs). The results showed that coffee ground-based CDs from coffee shops were water-soluble, light brown, and had an absorption peak at 300 nm. Nitrogen doping with urea increased the N content from 2,44% to 16,12%. The N and P significantly improved vegetative growth, grain yield, and seed quality, while N and K maintained chlorophyll in the reproductive stage. OM increased NPK efficiency and nutrient availability, with manure more effective than straw compost. CDs enhanced OM in increasing leaf area. The combination of NPK, OM, and CDs shows potential as an efficient and sustainable fertilization strategy to increase rice productivity and seed quality.
ABSTRAK
Carbon dots (CDs) adalah partikel karbon berukuran nano yang telah terbukti mampu meningkatkan perkecambahan benih, pertumbuhan, dan hasil tanaman. Penelitian ini bertujuan untuk mengevaluasi CDs dalam sistem pemupukan terintegrasi dengan pupuk hara makro (NPK) dan bahan organik. Penelitian dilaksanakan pada Musim Kemarau 2023 di lahan sawah irigasi Kebun Percobaan Sukamandi BBRMP Padi dengan Rancangan Petak-petak Terbagi. Kombinasi pupuk NPK (petak utama; tanpa NPK, +PK, +NP, +NK, +NPK), bahan organik (anak petak; pupuk kandang, kompos jerami, tanpa BO), dan aplikasi foliar CDs (800 mg L-1) (anak-anak petak; tanpa CDs, +CDs). Hasil percobaan menunjukkan CDs berbasis ampas kopi dari limbah coffee shop memiliki sifat larut air, berwarna cokelat terang, dan puncak absorbansi pada 300 nm. Pengkayaan nitrogen dengan urea meningkatkan kandungan N dari 2,44% menjadi 16,12%. Kombinasi N dan P sangat mempengaruhi pertumbuhan vegetatif, hasil gabah, dan mutu fisiologis benih. Kombinasi N dan K efektif dalam mempertahankan klorofil pada fase reproduktif. Bahan organik terbukti meningkatkan efisiensi pupuk NPK dan ketersediaan hara, dimana pupuk kandang lebih efektif dibandingkan kompos jerami. Aplikasi CDs meningkatkan efektivitas bahan organik dalam meningkatkan luas daun. Kombinasi pupuk NPK, bahan organik, dan CDs berpotensi menjadi strategi pemupukan efisien dan berkelanjutan untuk meningkatkan produktivitas dan mutu benih padi.
Kata kunci: Bahan organik, hasil, luas daun, pupuk NPK, sinergi pupuk
References
Ahmad, I., Jabeen, N., Ziaf, K., Dole, J. M., Khan, M. A. S., & Bakhtavar, M. A. (2017). Macronutrient application affects morphological, physiological, and seed yield attributes of calendula officinalis L. Canadian Journal of Plant Science, 97(5), 906–916. https://doi.org/10.1139/cjps-2016-0301
Blair, N., Faulkner, R. D., Till, A. R., Korschens, M., & Schulz, E. (2006). Long-term management impacts on soil C, N and physical fertility. Part II: Bad Lauchstadt static and extreme FYM experiments. Soil and Tillage Research, 91(1–2), 39–47. https://doi.org/10.1016/j.still.2005.11.001
Cai, D., Zhong, X., Xu, L., Xiong, Y., Deng, W., Zou, G., Hou, H., & Ji, X. (2025). Biomass-derived carbon dots: synthesis, modification and application in batteries. Chemical Science, 4937–4970. https://doi.org/10.1039/d4sc08659g
Costa, A. I., Barata, P. D., Moraes, B., & Prata, J. V. (2022). Carbon dots from coffee grounds: synthesis, characterization and detection of noxious nitroanilines. Chemosensors, 10(3), 1–17. https://doi.org/10.3390/chemosensors10030113
Crista, D. M. A., El Mragui, A., Algarra, M., Esteves da Silva, J. C. G., Luque, R., & da Silva, L. P. (2020). Turning spent coffee grounds into sustainable precursors for the fabrication of carbon dots. Nanomaterials, 10(6), 1–17. https://doi.org/10.3390/nano10061209
Dobermann, A., & Fairhurst, T. (2000). Rice: Nutrient Disorders and Nutrient Management. In International Rice Research Institute, of Canada Potash and Phospate Institute (Issue 3). Oxford Graphic Printers Pte Ltd.
Fawaz, W., Hasian, J., & Alghoraibi, I. (2023). Synthesis and physicochemical characterization of carbon quantum dots produced from folic acid. Scientific Reports, 13(1), 1–7. https://doi.org/10.1038/s41598-023-46084-1
Hampton, J. G., Boelt, B., Rolston, M. P., & Chastain, T. G. (2013). Effects of elevated CO2 and temperature on seed quality. Journal of Agricultural Science, 151(2), 154–162. https://doi.org/10.1017/S0021859612000263
Havlin, J. L., Tisdale, S. L., Nelson, W. L., & Beaton, J. D. (2017). Soil Fertility and Fertilizers. An Introduction to Nutrient Management. In Rules of Thumb for Petroleum Engineers (Eighth Edi). Pearson India Education Services Pvt. Ltd. https://doi.org/10.1002/9781119403647.ch8
Huang, B., Yuan, Z., Li, D., Zheng, M., Nie, X., & Liao, Y. (2020). Effects of soil particle size on the adsorption, distribution, and migration behaviors of heavy metal(loid)s in soil: A review. Environmental Science: Processes and Impacts, 22(8), 1596–1615. https://doi.org/10.1039/d0em00189a
Isnaeni, I., Purwandari, V., Putro, P. A., & Adiwidya, H. M. (2023). Optical properties of sodium-doped carbon dots made of urea and trisodium citrate. TIME in Physics, 1(1), 1–9. https://doi.org/10.11594/timeinphys.2023.v1i1p1-9
ISTA. (2018). International Rules for Seed Testing 2018. In International Rules for Seed Testing (Issues i-19-8 (298)). International Seed Testing Association. https://doi.org/10.15258/istarules.2018.f
Jamil, A., Abdulrachman, S., & Syam, M. (2014). Dinamika anjuran dosis pemupukan N, P, dan K pada padi sawah. IPTEK Tanaman Pangan, 9(2), 63–77.
Janissen, B., & Huynh, T. (2018). Chemical composition and value-adding applications of coffee industry by-products : A review. 128(July 2017), 110–117. https://doi.org/10.1016/j.resconrec.2017.10.001
Jeong, G., Park, C. H., Yi, D., & Yang, H. (2023). Green synthesis of carbon dots from spent coffee grounds via ball-milling: Application in fluorescent chemosensors. Journal of Cleaner Production, 392(15), 1–10.
Kalaji, H. M., Oukarroum, A., Alexandrov, V., Kouzmanova, M., Brestic, M., Zivcak, M., Samborska, I. A., Cetner, M. D., Allakhverdiev, S. I., & Goltsev, V. (2014). Plant physiology and biochemistry identification of nutrient deficiency in maize and tomato plants by in vivo chlorophyll a fluorescence measurements. Plant Physiology and Biochemistry, 81, 16–25. https://doi.org/10.1016/j.plaphy.2014.03.029
Kang, C., Huang, Y., Yang, H., Yan, X. F., & Chen, Z. P. (2020). A review of carbon dots produced from biomass wastes. Nanomaterials, 10(11), 1–24. https://doi.org/10.3390/nano10112316
Kolasinska, K., Szyrmer, J., & Dul, S. (2000). Relationship between laboratory seed quality tests and field emergence of common bean seed. Crop Science, 40(2), 470–475. https://doi.org/10.2135/cropsci2000.402470x
Li, H., Huang, J., Lu, F., Liu, Y., Song, Y., Sun, Y., Zhong, J., Huang, H., Wang, Y., Li, S., Lifshitz, Y., Lee, S. T., & Kang, Z. (2018). Impacts of carbon dots on rice plants: Boosting the growth and improving the disease resistance. ACS Applied Bio Materials, 1(3), 663–672. https://doi.org/10.1021/acsabm.8b00345
Li, Y., Xu, X., Wu, Y., Zhuang, J., Zhang, X., Zhang, H., Lei, B., Hu, C., & Liu, Y. (2020). A review on the effects of carbon dots in plant systems. Materials Chemistry Frontiers, 4(2), 437–448. https://doi.org/10.1039/c9qm00614a
Ma, P., Lan, Y., Lv, X., Fan, P., Yang, Z., Sun, Y., Zhang, R., & Ma, J. (2021). Reasonable nitrogen fertilizer management improves rice yield and quality under a rapeseed/wheat–rice rotation system. Agriculture (Switzerland), 11(6). https://doi.org/10.3390/agriculture11060490
Moe, K., Htwe, A. Z., Thu, T. T. P., Kajihara, Y., & Yamakawa, T. (2019). Effects on NPK status, growth, dry matter and yield of rice (Oryza sativa) by organic fertilizers applied in field condition. Agriculture, 9(5). https://doi.org/10.3390/agriculture9050109
Mu, X., & Chen, Y. (2021). The physiological response of photosynthesis to nitrogen deficiency. Plant Physiology and Biochemistry, 158(November 2020), 76–82. https://doi.org/10.1016/j.plaphy.2020.11.019
Naher, U. A., Ahmed, M., Sarkar, M., Biswas, J., & Panhwar, Q. A. (2019). Fertilizer management strategies for sustainable rice production. In S. Chandran, M. Unni, & S. Thomas (Eds.), Organic Farming: Global Perspectives and Methods (pp. 251–267). Elsevier Inc. https://doi.org/10.1016/B978-0-12-813272-2.00009-4
Nugraha, U. S. (2004). Legislasi, kebijakan, dan kelembagaan pembangunan perbenihan. Perkembangan Teknologi Tro., 16(1), 61–75.
Pan, Z., Zang, H., Li, Y., Wang, X., Xia, N., Liu, C., Li, Z., Han, Y., Tang, Z., & Sun, J. (2024). Foliar application of carbon dots enhances nitrogen uptake and assimilation through CEPD1-dependent signaling in plants. Plant Physiology and Biochemistry, 217, 109229. https://doi.org/10.1016/j.plaphy.2024.109229
Papadopoulos, A., Bird, N. R. A., Whitmore, A. P., & Mooney, S. J. (2014). Does organic management lead to enhanced soil physical quality? Geoderma, 213, 435–443. https://doi.org/10.1016/j.geoderma.2013.08.033
Peng, J., Feng, Y., Wang, X., Li, J., Xu, G., Phonenasay, S., Luo, Q., Han, Z., & Lu, W. (2021). Effects of nitrogen application rate on the photosynthetic pigment, leaf fluorescence characteristics, and yield of indica hybrid rice and their interrelations. Scientific Reports, 11(1), 1–10. https://doi.org/10.1038/s41598-021-86858-z
Porra, R. J., Thompson, W. A., & Kriedemann, P. E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assayong chlorophylls a and b extracted with four different solvents: verification of tje concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta, 975, 384–394.
Pratiwi, R. A., & Nandiyanto, A. B. D. (2022). How to read and interpret UV-VIS spectrophotometric results in determining the structure of chemical compounds. Indonesian Journal of Educational Research and Technology, 2(1), 1–20. https://doi.org/10.17509/ijert.v2i1.35171
Sadjad, S. (1993). Dari Benih Kepada Benih. PT. Gramedia Widiasarana Indonesia.
Salsabilla, U. S., Kusumawati, D. H., & Fitriana. (2024). Karakteristik carbon dots (C-dots) dari buah jeruk mandarin (Citrus reticulata) yang disintesis menggunakan metode hidrotermal. Jurnal Inovasi Fisika Indonesia (IFI), 13, 60–73.
Serrano, J., da Silva, J. M., Shahidian, S., Silva, L. L., Sousa, A., & Baptista, F. (2017). Differential vineyard fertilizer management based on nutrient’s spatio-temporal variability. Journal of Soil Science and Plant Nutrition, 17(1), 46–61. https://doi.org/10.4067/S0718-95162017005000004
Sharifi, S., Shi, S., Obaid, H., Dong, X., & He, X. (2024). Differential Effects of Nitrogen and Phosphorus Fertilization Rates and Fertilizer Placement Methods on P Accumulations in Maize. Plants, 13(13). https://doi.org/10.3390/plants13131778
Song, J., Yang, H., Yu, X., Chen, Y., Yang, C., He, Y., & Wang, H. (2025). Effects of combined application of nitrogen, phosphorus, and potassium fertilizers on seed yield, seed quality and economic returns of Elymus nutans in alpine region. BMC Plant Biology, 25(1). https://doi.org/10.1186/s12870-025-06126-4
Suman, A., Singh, K. P., Singh, P., & Yadav, R. L. (2009). Carbon input, loss and storage in sub-tropical Indian Inceptisol under multi-ratooning sugarcane. Soil and Tillage Research, 104(2), 221–226. https://doi.org/10.1016/j.still.2009.02.008
Sunoqrot, S., Al-Shalabi, E., Al-Bakri, A. G., Zalloum, H., Abu-Irmaileh, B., Ibrahim, L. H., & Zeno, H. (2021). Coffee bean polyphenols can form biocompatible template-free antioxidant nanoparticles with various sizes and distinct colors. ACS Omega, 6(4), 2767–2776. https://doi.org/10.1021/acsomega.0c05061
Taiz, L., & Zeiger, E. (2002). Plant Physiology. Third Edition. In Science progress (Vol. 34, Issue 136). Sinauer Associates. https://doi.org/10.1017/9781108486392
Triwardiati, D., & Ermawati, I. R. (2018). Analisis bandgap karbon nanodots (C-Dots) kulit bawang merah menggunakan teknik microwave. Prosiding Seminar Nasional Teknoka, 3(2502), 25. https://doi.org/10.22236/teknoka.v3i0.2810
Wang, H., Zhang, M., Song, Y., Li, H., Huang, H., Shao, M., Liu, Y., & Kang, Z. (2018). Carbon dots promote the growth and photosynthesis of mung bean sprouts. Carbon, 136, 94–102. https://doi.org/10.1016/j.carbon.2018.04.051
Weil, R. R., & Brady, N. C. (2016). The Nature and Properties of Soils (15th Editi, Issue May). Pearson Education, Inc.
Yan, F., Jang, Y., Sun, X., Wei, J., Chen, L., & Zhang, Y. (2019). Multicolor carbon dots with concentration-tunable fluorescence and solvent-affected aggregation states for white light-emitting diodes. Nano Research, 13, 52–60. https://doi.org/https://doi.org/10.1007/s12274-019-2569-3
Zangani, E., Afsahi, K., Shekari, F., Sweeney, E. Mac, & Mastinu, A. (2021). Nitrogen and phosphorus addition to soil improves seed yield, foliar stomatal conductance, and the photosynthetic response of rapeseed (Brassica napus l.). Agriculture (Switzerland), 11(6). https://doi.org/10.3390/agriculture11060483
Zhang, F., & Zhou, G. (2019). Estimation of vegetation water content using hyperspectral vegetation indices: A comparison of crop water indicators in response to water stress treatments for summer maize. BMC Ecology, 19(1), 1–12. https://doi.org/10.1186/s12898-019-0233-0
Zhao, L., Li, K., Wang, Q., Song, X., Su, H., & Xie, B. (2017). Nitrogen starvation impacts the photosynthetic performance of porphyridium cruentum as revealed by chlorophyll a fluorescence. Scientific Reports, 7, 8542. https://doi.org/10.1038/s41598-017-08428-6
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