Aplikasi Bacillus sp. untuk mengendalikan penyakit layu fusarium pada tanaman tomat
DOI:
https://doi.org/10.15575/5397Keywords:
Bacillus sp., Fusarium oxysporum, pengendalian hayati, tomatAbstract
Fusarium oxysporum merupakan penyebab penyakit layu fusarium yang dapat menurunkan produksi tomat. Pengendalian hayati dengan menggunakan bakteri antagonis seperti Bacillus sp. merupakan alternatif pengendalian yang potensial dan ramah lingkungan. Penelitian bertujuan untuk mengetahui kemampuan Bacillus sp. dalam mengendalikan penyakit layu fusarium pada tanaman tomat di lapangan. Percobaan menggunakan rancangan acak kelompok dengan 5 perlakuan dan 5 ulangan, meliputi: kontrol, Bacillus sp. B42, Bacillus sp. B64, gabungan Bacillus sp. B42 dan B64, serta fungisida. Hasil pengujian menunjukkan bahwa Bacillus sp. B.64 merupakan bakteri antagonis terbaik dalam menekan intensitas penyakit layu fusarium, karena dapat menunda masa inkubasi 15,76%, menekan intensitas penyakit 38,77%, meningkatkan kandungan fenol tanaman (tanin, saponin dan glikosida), serta meningkatkan pertumbuhan dan hasil tanaman dengan meningkatkan bobot kering tajuk 32,05%, bobot kering akar 15,23%, dan bobot buah per tanaman 46,48%.
ABSTRACT
Fusarium oxysporum is the causal agent of fusarium wilt disease which decreases the production of tomatoes. Biological control managenement using bacterial antagonists is a potential alternative to prevent the infection of the disease. The aim of this research was to determine the ability of Bacillus sp. to control tomato fusarium wilt in the field. Randomized block design (RBD) experiment was used consisting of 5 treatments and 5 replications i.e. control, Bacillus sp. B42, Bacillus sp. B64, combination of Bacillus sp B42 + B64, and fungicide. The results showed that Bacillus sp. B64 was the best bacterial antagonist agent to control tomato wilt disease by delaying incubation period (15.76%), decreasing disease intensity (38.77%), increasing phenol compounds (tannin, saponin, glycosides) and improving plant growth and yield. Furthermore, the results showed the increasement of shoot dry weight to 32.05%, root dry weight to 15.23%, and yield to 46.48% as well.
References
Abed, H., Rouag, N., Mouatassem D., & Rouabhi, A. (2016). Screening for Pseudomonas and Bacillus antagonistic rhizobacteria strains for the biocontrol of Fusarium wilt of chickpea. Eurasian J Soil Sci 5 (3):182–191. http://dx.doi.org/10.18393/ejss.2016.3.182-191
Abo-Elyours, K.A.M., & Mohamed, H.M. (2009). Biological control of Fusarium wilt in tomato by plant growth-promoting yeast and rhizobacteria. Plant Pathol. J. 25(2): 199-204. http://dx.doi.org/10.5423/PPJ.2009.25.2.199
Akram, W. & Anjum, T. (2011). Quantitative changes in defense system of tomato induced by two strain of Bacillus against Fusarium wilt. Indian Journal of Fundamental and Applied Life Sciences, 1(3): 7-13.
Cawoy, H., Bettiol, W., Fickers, P. & Ongena, M. (2011). Bacillus-based biological control of plant diseases. In. Stoycheve M. (ed.). Pesticides in the modern world, pesticides use and management. pp.273-302. Intech Europe, Croatia.
Chaerul. (2003). Identifikasi secara cepat bahan bioaktif pada tumbuhan di lapangan. Berita Biologi 6(4):621-628.
Chi-Yea, Y., Yi-Cheng, H., Jen-Chieh, P., Shiang-Suo, H., & Seng-
Ming, T.J. (2009). Cloning and expression of an antifungal chitinase gene of a novel Bacillus subtilis isolate from Taiwan potato field. Bioresource Technology 100(3):1454-1458.
http://dx.doi.org/10.1016/j.biortech.2008.07.039
Compant, S.B., Duffy, Nowak, J., Clement, C., & Barka E.A. (2005). Use of plant growth-promotng bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Applied and Enviromental Microbiology, 71(9): 4951-4959. http://dx.doi.org/10.1128/AEM.71.9.4951–4959.
Foster K.R., & Bell, T. (2012). Competition, not cooperation, dominates interactions among culturable microbial spesies. Current Biology 22(19): 1845-1850. http://dx.doi.org/10.1016/j.cub.2012.08.005.
Ghasemi, S., Gholamreza, A., Nadali, J., Heshmatollah, R., Soheila, G., Ali, D., & Parvin, S. (2010). Antifungal chitinases from Bacillus pumilus SG2: preliminary report . World Journal of Microbiology & Biotechnology 26(8):1437-1443. : http://dx.doi.org/10.1007/s11274-010-0318-6
Gomaa, E.Z. (2012). Chitinase Production by Bacillus thuringiensis and Bacillus licheniformis: Their Potential in Antifungal Biocontrol. The Journal of Microbiology, 50(1) : 103–111. http://dx.doi.org/10.1007/s12275-012-1343-y
Gond, S.K., Bergena, M.S., Torresa, M.S., & White, J.F.Jr. (2015). Endophytic Bacillus spp. produce antifungal lipopeptides and induce host defence gene expression in maize. Microbiological Research, 172:79–87. http://dx.doi.org/10.1016/j.micres.2014.11.004
Hyakumachi, M., Nishimura, M., Arakawa, T., Asano, S., Yoshida, S., Tsushima, S., & Takahashi, H. (2013). Bacillus thuringiensis suppresses bacterial wilt disease caused by Ralstonia solanacearum with systemic induction of defense-related gene expression in tomato. Microbes Environ., 28 (1):128–134.
Javandira, C., Aini, L.Q., Sugiharto, A.N., & Abadi, A.L. (2013). The potency of Bacillus sp. and Pseudomonas sp. as biological control agents against corn leaf blight disease caused by Pantoea sp. Agrivita, 35(2):103-109. http://dx.doi.org/10.17503/Agrivita-2013-35-2-p103-109
Kloepper, J.W., Rodriguez-Ubana, R., Zehnder, G.W., Murphy, J.F., Sikora, E., & Fernandez, C. (1999). Plant root-bacterial interactions in biological control of soilborne diseaes and potential extension to systemic and foliar diseases. Australian Plant Pathology, 28:21-26. http://dx.doi.org/10.1071/AP99003.
Malfanova, N.V. (2013). Endophytic Bacteria with Plant Growth Promoting and Biocontrol Abilities. Leiden University Repository. Hal: 15-37.
Moeinzadeh, A., Sharif-Zadeh, F., Ahmadzadeh, M., & Heidari-Tajabadi, F. (2010). Biopriming of sunflower (Helianthus annuus L.) seed with Pseudomonas fluorescens for improvement of seed invigoration and seedling growth. Australian Journal of Crop Science, 4(7):564-570
Mugiastuti, E, & Rahayuniati, R.F. (2014). Perakitan biopestisida berbasis mikroba untuk mengendalikan penyakit utama tanaman tomat di Kabupaten Banyumas. Laporan Penelitian. LPPM Universitas Jenderal Soedirman, Purwokerto.
Prasanna-Reddy, B, & Rao, K.S. (2009). Biochemical and PCR_PAPD characterization of Pseudomonas fluorescens produced antifungal compounds inhibit the rice fungal pathogens in vitro. Electronic Journal of Enviromental, Agricultural and Food Chemistry, 8(10): 1062-1067.
Radhakrishnan, R., & Lee, I. (2016). Gibberellins producing Bacillus methylotrophicus KE2 supports plant growth and enhances nutritional metabolites and food values of lettuce. Plant Physiology and Biochemistry, 109: 181-189. http://dx.doi.org/10.1016/j.plaphy.2016.09.018
Shinde, A.A, Shaikh, F.K., Padul, M.V., & Kachole, M.S. (2012). Bacillus subtillis RTSBA6 6.00, a new strain isolated from gut of Helicoverpa armigera (Lepidoptera: Noctuidae) produces chymotrypsin-like proteases. Saudi Journal of Biological Sciences, 19(3):317–323. http://dx.doi.org/10.1016/j.sjbs.2012.03.001
Soesanto, L. Mugiastuti, E., Manan, A., & Wachjadi, M. (2013). Ability test of several antagonists to control potato bacterial wilt in the field. Agrivita, 35 (1):30-35. http://dx.doi.org/10.17503/Agrivita-2013-35-1-p030-035.
Vijayalakshmi, S., Ranjitha, J., & Rajeswari, V D. (2013). Enzyme production ability by Bacillus subtilis and Bacillus licheniformis - A comparative study. Asian Journal of Pharmaceutical and Clinical Research, 6(4): 29-32
Wiryanta, B.T.W. (2002). Bertanam Tomat. Agromedia Pustaka, Jakarta. 100 hal
Zalila-Kolsi, I., Mahmoud, A.B., Ali, H., Sellami, S., Nasfi, Z., Tounsi, S. & Jamoussi, K. (2016). Antagonist effects of Bacillus spp. strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L. subsp. durum). Microbiological Research 192:148–158. http://dx.doi.org/10.1016/j.micres.2016.06.012
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