Characterization of Secondary Metabolites and Cytotoxic Assay of Haliclona sp. Sponge Against T47D Breast Cancer Cells


Ajuk Sapar(1*), Millenia Millenia(2), Anthoni Batahan Aritonang(3), Rudiyansyah Rudiyansyah(4), Winda Rahmalia(5)

(1) Department of Chemistry, Tanjungpura University, Indonesia
(2) Department of Chemistry, Tanjungpura University, Indonesia
(3) Department of Chemistry, Tanjungpura University, Indonesia
(4) Department of Chemistry, Tanjungpura University, Indonesia
(5) Department of Chemistry, Tanjungpura University, Indonesia
(*) Corresponding Author

Abstract


Characterization of secondary metabolites and cytotoxic testing of Haliclona sp. against T47D breast cancer cells were conducted in this study. The objective was to assess the cytotoxicity of T47D cancer cells and identify the functional groups involved. The research methods employed included maceration, partitioning, phytochemical testing, toxicity testing using the BSLT method, separation through flash column chromatography (FCC), cytotoxic testing using the MTT method, and characterization using FTIR. The partition results of methanol extract consist of n-hexane, ethyl acetate, and methanol-water fractions. The methanol extract demonstrated high toxicity, with an LC50 of 5.21 ppm. Among the fractions, the ethyl acetate fraction exhibited the highest toxicity compared to the n-hexane and methanol-water fractions, with LC50 values of 25.76 ppm, 42.71 ppm, and 55.26 ppm, respectively. Phytochemical testing of the ethyl acetate fraction yielded positive results for terpenoids, steroids, alkaloids, and phenolic compounds. The ethyl acetate fraction was further separated using flash column chromatography, resulting in ten combined fractions (M1-M10). The cytotoxicity tests of the M3 fraction against 747D breast cancer cells showed non-toxic effects, with an IC50 value of 1382.29 ppm. The FTIR analysis of the M3 fraction revealed the presence of functional groups such as O-H, =C-H, C-H aliphatic, C=O, and C=C, which is indicative of the presence of terpenoids, steroids, and esters.

Keywords


Anticancer; secondary metabolites; cytotoxic; Haliclona sp. sponge; T47D cells

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References


Maushmi S. Kumar and Asim K. Pal, “A review of bioactive compounds from marine organisms with special mention on the potential of marine sponges in pharmacological applications”, J. Mar. Biol. Ass. India, 58(1), 2016.

S. Sujatha, G.Prakash Vincent, and M. Jobi Dhas, “Marine sponge and its potential bioactive spectrum against CSF-affected bacterial organisms”, The Pharma Innovation Journal, 3(6), 81-86, 2014.

S.B. Helber, D.J.J. Hoeijmakers, C.A. Muhando, S. Rohde, P.J. Schupp, “Sponge chemical defenses are a possible mechanism for increasing sponge abundance on reefs in Zanzibar”, PLoS ONE, 13(6), e0197617, 2018, doi: 10.1371/journal.pone.019761.

A.C. Abraham, D.J. Gochfeld, K. Macartney, A. Mellor, M.P. Lesser, and M. Slattery, “Biochemical variability in sponges across the Caribbean basin”, Invertebrate Biology, 140(3), e12341, 2021, doi: 10.1111/ivb.12341.

C. Calcabrini, E. Catanzaro, A. Bishayee, E. Turrini, and C. Fimognari. “Marine sponge natural products with anticancer potential: An updated review. Mar. Drugs, 15(10), 310, 2017, doi: 10.3390/md15100310.

H. Zhang, M. Dong, J. Chen, H. Wang, K. Tenney, and P. Crews, “Review: bioactive secondary metabolites from the marine sponge genus agelas”, Mar. Drugs, 15(11), 351, 2017, doi: 10.3390/md15110351.

A.M Elissawy, E.S. Dehkordi, N. Mehdinezhad, M.L. Ashour, P.M. Pour, “Cytotoxic alkaloids derived from marine sponges: A comprehensive review”, Biomolecules, 11(2), 258, 2021, doi: 10.3390/biom11020258.

I.M.D. Swantara, W.S. Rita, and A. Hernindya, "Identifikasi isolat antikanker spons Hyrtios erecta", Indonesian Journal of Cancer, 10(4), 123-129, 2016, doi: 10.33371/ijoc.v10i4.456.

M.R. Kurniawan, A. Sapar, and A.B. Aritonang, "Úji toksisitas dan uji fitokimia spons Haliclona sp. asal pulau lemukutan kabupaten bengkayang kalimantan barat", Jurnal Kimia Khatulistiwa, 9(1), 2303-1077, 2021.

M. Sangi, M.R.J. Runtuwene, H.E.I. Simbala dan V.M.A. Makang, "Analisis fitokimia tumbuhan obat di kabupaten minahasa utara", Chemistry Progress, 1, 47-53, 2008.

Suciati, and L. Arifianti. “In vitro anticancer activity of marine sponges against T47D and hela cell lines”. Dhaka Univ. J. Pharm. Sci., 19(1), 25-28, 2020.

W.M. Alarif, A. Abdel-Lateff, S.S. Al-Lihaibi, S.E.N. Ayyad, and F.A. Badria, “A new cytotoxic brominated acetylenic hydrocarbon from the marine sponge Haliclona sp. with a selective effect against human breast cancer”, Z. Naturforsch C J Biosci, 68(1-2), 70 – 75 2013.

J.P. Houghton and A. Raman, "Laboratory Handbook for The Fractionation of Natural Extracts", ITP, London, 102-105, 1998.

I.G.I.P. Putra, “Uji aktivitas antikanker ekstrak etanol biji srikaya (Annona squamosa L.) terhadap beberapa sel kanker manusia secara in vitro”, Surabaya, Universitas Airlangga, 2015.

L. Puspitasari, D.A. Swastini, and C.I.S. Arisanti, “Skrining fitokimia ekstrak etanol 95% kulit buah manggis (Gracinia mangostana L.)”, Jurnal Farmasi Udayana, 2(3), 1-5, 2013.

R. Setyawaty, R. Aptuning A.B. Dewanto, “Preliminary studies on the content of phytochemical compounds on skin of salak fruit (salaccazalacca)”, Pharmaceutical Journal of Indonesia, 6(1), 1-6, 2020, doi: 10.21776/ub.pji.2020.006.01.1.

N. Kancherla, A. Dhakshinamooth, K. Chitra, and R.B. Komaram, “Preliminary analysis of phytoconstituents and evaluation of anthelminthic property of Cayratia auriculata (in vitro)”, MAEDICA – a Journal of Clinical Medicine, 14(4), 350-356, 2019, doi: 10.26574/maedica.2019.14.4.350.

T. Saptawati, E. Dayanti, F.A. Rachma, and Ovikariani, “Phytochemical screening of ethanol extract of trembesi fruit seeds (Samanea saman)”, Science and Community Pharmacy Journal, 1(2), 69-77, 2022.

J.P. Sinurat, V. Krisdianilo, R.M.br. Karo, and R. Berutu. “Analysis of total terpenoids from Maniltoa Grandiflora (A. Gray) scheff leaves using TLC and HPLC methods”, Stannum: Jurnal Sains dan terapan Kimia, 2(2), 40-44, 2020, doi: 10.33019/jstk.v2i2.1976.

R. Mutiah, A.L. Inayatin, R. Annisa, Y.Y.A. Indrawijaya, and A. Listiyana, “Inhibition of cell cycle and induction of apoptosis y ethanol leaves extract of Chrysanthemum cinerariifolium (Trev.) In T47D breast cancer cells”, Indonesian Journal of Pharmacy, 31(1), 1–10, 2020, doi: 10.14499/indonesianjpharm31iss1pp1.

D. Handayani, W. Rasyid, Rustini, E.N. Zainudin, and T. Hertiani, “Cytotoxic activity screening of fungal extracts derived from the west sumatran marine sponge Haliclona fascigera to several human cell lines: hela, WiDr, T47D, and Vero”, Journal of Applied Pharmaceutical Science, 8(01), 055-058, 2018, doi: 10.7324/JAPS.2018.8109.

W. Woradulayapinij, A. Pothiluk, T. Nualsanit, T. Yimsoo, W. Yingmema, P. Rojanapanthu, Y. Hong, S.J. Baek, and W. Treesuppharat, “Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis”, Toxicology Reports, 9, 1968-1976, 2022, doi: 10.1016/j.toxrep.2022.10.015.

S. Safarpour, S. Safarpour, M. Pirzadeh, A. A. Moghadamnia, A. Ebrahimpour, F. Shirafkan, R. Mansoori, S. Kazemi, and M. Hosseini, “Colchicine ameliorates 5-fluorouracil-induced cardiotoxicity in rats”, Oxidative Medicine and Cellular Longevity, 2022, doi: 10.1155/2022/6194532.

S. Susilowati, A. Claresa, and I. Arifin, “Uji sitotoksik fraksi n-heksana ekstrak etanol herba alfaalfa (Medicago Sativa L.) pada sel T47D dan sel HeLa serta identifikasi kandungan senyawa kimianya”, Media Farmasi Indonesia, 9(2), 2012, doi: 10.31942/jiffk.v9i2.858.

S. Moeljopawiro, M.R. Anggelia D., B. Ayuningtyas, Y. Widaryanti, Sari, and I.M. Budi, “Pengaruh sari buah merah (Pandanus conoideus Lam.) terhadap pertumbuhan sel kanker payudara dan sel kanker usus besar”, Berkala Ilmiah Biologi, 6(2), 121-130, 2007.




DOI: https://doi.org/10.15575/ak.v10i1.20334

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