Synthesis, Characterization of Polyvinyl Alcohol-Chitosan-ZnO/CuO Nanoparticles Film and Its Biological Evaluation as An Antibacterial Agent of Staphylococcus aureus

Authors

  • Ahmad Fatoni Department of Pharmacy, Bhakti Pertiwi College of Pharmacy, Indonesia
  • Mauizatul Hasanah Department of Pharmacy, Bhakti Pertiwi College of Pharmacy, Indonesia
  • Lasmaryna Sirumapea Department of Pharmacy, Bhakti Pertiwi College of Pharmacy, Indonesia
  • Annisa Defanie Putri Department of Pharmacy, Bhakti Pertiwi College of Pharmacy, Indonesia
  • Khoirunnisa Sari Department of Pharmacy, Bhakti Pertiwi College of Pharmacy, Indonesia
  • Restu Dwi Khairani Department of Pharmacy, Bhakti Pertiwi College of Pharmacy, Indonesia
  • Nurlisa Hidayati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indonesia

DOI:

https://doi.org/10.15575/ak.v10i1.24725

Keywords:

PVA-chitosan-ZnO/CuO nanoparticles film, characterization, Staphylococcus aureus

Abstract

The polyvinyl alcohol-chitosan-ZnO/CuO nanoparticles film was researched. Synthesis, characterization, and its biological evaluation as an antibacterial of Staphylococcus aureus were the aims of this research. The biosynthesis of ZnO, CuO, and ZnO/CuO nanoparticles was done using the biological method. The polyvinyl alcohol-chitosan-ZnO/CuO nanoparticles film was synthesized using the casting method. All the products were characterized by FTIR spectroscopy, X-ray diffraction, and Scanning Electron Microscope (SEM). Polyvinyl alcohol-chitosan-ZnO/CuO nanoparticles film as a paper disk for the evaluation as an antibacterial agent through the agar disk diffusion method. The absorption bands of ZnO, CuO, and ZnO/CuO nanoparticles can be observed at 318, 274, and 252 nm, respectively. The peaks at wavenumbers 433-673 and 619 cm-1 were Zn-O and Cu-O groups, respectively. The Zn-O and Cu-O groups at ZnO/CuO nanoparticles can be observed at 474 and 619 cm-1. The appearance of Zn-O and Cu-O groups at film PVA-chitosan-ZnO/CuO nanoparticles indicates the wavenumber between 433 and 673 cm-1. The physical structure of ZnO, CuO, and ZnO/CuO nanoparticles is crystalline form. The crystallite size of ZnO, CuO, and ZnO/CuO nanoparticles was estimated at 1.0572, 6.6315, and 2.3333 nm respectively. The physical structure of film PVA-chitosan-ZnO/CuO nanoparticles is amorphous. The surface morphology of films C, D, and E was affected by the addition of chitosan and ZnO/CuO nanoparticles. The film of PVA-chitosan-ZnO/CuO nanoparticles (C, D and E) can act as an antibaterial agent of Staphylococcus aureus.The inhibition zone of film D is higher than A, B, C, and E.

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2023-06-30

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Vol. 10 No. 1 (2023): al Kimiya: Jurnal Ilmu Kimia dan Terapan

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