Urine Glucose Detection Via Gold Nanoparticle Formation Using 3D-Connector Microfluidic Paper Based Analytical Devices

Authors

  • Krista Firdaus Suwarno Putri Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Brawijaya, Indonesia
  • Hermin Sulistyarti Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Brawijaya, Indonesia
  • Akhmad Sabarudin Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Brawijaya, Indonesia

DOI:

https://doi.org/10.15575/ak.v11i1.35245

Keywords:

Diabetes mellitus, glucose, μPADs, AuNPs, non-invasive

Abstract

A metabolic disorders that have experienced a significant increase in the world are diabetes mellitus. Diabetes is caused by two main factors: the first is damage to pancreatic beta cells, which prevents insulin from being produced, and the second is impaired insulin secretion and function. Chronic diabetes, if not treated properly, can lead to acute complications including eye, kidney, lung, nerve, and even death. Diabetes can be diagnosed through blood and urine. In general, glucose detection is carried out using invasive methods that use blood samples, which can cause pain and discomfort for users. Current research is developing non-invasive glucose detection using urine samples. This research aims to develop non-invasive glucose detection technology using 3D-connector μPADs (Microfluidic Paper Based Analytical Devices) which have the advantages of being safe, easy, and simple. The three-dimensional connector on the device functions as a connector to facilitate the coordination of fluid flow in the sample zone and detection zone. The glucose detection method uses gold (III) chloride as a gold nanoparticle (AuNPs) precursor, an aqueous extract of Acalypha indica Linn as a stabilizing agent, sodium hydroxide (NaOH) as a catalyst, and glucose in artificial urine as a sample. Method validation results using imageJ software indicated linearity with a coefficient of determination value (R2) of 0.9714, precision with a %RSD value (Relative Standard Deviation) of 2.69, and an accuracy level ranging from 92.22-99.23%.

References

Z. Punthakee, R. Goldenberg, and P. Katz, “Definition, classification and diagnosis of diabetes, prediabetes and metabolic syndrome,†Can J Diabetes, 42, S10–S15, Apr. 2018, https://doi.org/10.1016/j.jcjd.2017.10.003

O. A. Ojo, H. S. Ibrahim, D. E. Rotimi, A. D. Ogunlakin, and A. B. Ojo, “Diabetes mellitus: From molecular mechanism to pathophysiology and pharmacology,†Sep. 01, 2023, https://doi.org/10.1016/j.medntd.2023.100247

A. T. Kharroubi, “Diabetes mellitus: The epidemic of the century,†World J Diabetes, 6(6), 850, 2015,

https://doi.org/10.4239/wjd.v6.i6.850

X. Lin et al., “Global, regional, and national burden and trend of diabetes in 195 countries and territories: an analysis from 1990 to 2025,†Sci Rep, 10(1), Dec. 2020,

https://doi.org/10.1038/s41598-020-71908-9

M. A. B. Khan, M. J. Hashim, J. K. King, R. D. Govender, H. Mustafa, and J. Al Kaabi, “Epidemiology of type 2 diabetes - global burden of disease and forecasted trends,†J Epidemiol Glob Health, 10(1), 107–111, Mar. 2020,

https://doi.org/10.2991/jegh.k.191028.001

W. V. Gonzales, A. T. Mobashsher, and A. Abbosh, “The progress of glucose monitoring—A review of invasive to minimally and non-invasive techniques, devices and sensors,†Sensors, 19(800), 1–45, Feb. 2019,

https://doi.org/10.3390/s19040800

K. Khachornsakkul, F. J. Rybicki, and S. Sonkusale, “Nanomaterials integrated with microfluidic paper-based analytical devices for enzyme-free glucose quantification,†Talanta, 260(124538), 1–9, Aug. 2023,

https://doi.org/10.1016/j.talanta.2023.124538

S. Liu, W. Su, and X. Ding, “A review on microfluidic paper-based analytical devices for glucose detection,†MDPI AG. Dec. 01, 2016,

https://doi.org/10.3390/s16122086

J. Sun, Y. Xianyu, and X. Jiang, “Point-of-care biochemical assays using gold nanoparticle-implemented microfluidics,†Royal Society of Chemistry, Sep. 07, 2014,

https://doi.org/10.1039/C4CS00125G

O. Tokel, F. Inci, and U. Demirci, “Advances in plasmonic technologies for point of care applications,†American Chemical Society, Jun. 11, 2014,

https://doi.org/10.1021/cr4000623

T. Tian, J. Li, Y. Song, L. Zhou, Z. Zhu, and C. J. Yang, “Distance-based microfluidic quantitative detection methods for point-of-care testing,†Royal Society of Chemistry, Apr. 07, 2016,

https://doi.org/10.1039/C5LC01562F

T. Pinheiro et al., “Paper-based in-situ gold nanoparticle synthesis for colorimetric, non-enzymatic glucose level determination,†Nanomaterials, 10(10), 1–20, Oct. 2020,

https://doi.org/10.3390/nano10102027

T. Pinheiro, A. C. Marques, P. Carvalho, R. Martins, and E. Fortunato, “Paper microfluidics and tailored gold nanoparticles for nonenzymatic, colorimetric multiplex biomarker detection,†ACS Appl Mater Interfaces, 13(3), 3576–3590, Jan. 2021,

https://doi.org/10.1021/acsami.0c19089

L. Yang, Z. Zhang, and X. Wang, “A microfluidic PET-based electrochemical glucose sensor,†Micromachines (Basel), 13(552), 1–8, Apr. 2022,

https://doi.org/10.3390/mi13040552

Z. Liu, F. Zhao, S. Gao, J. Shao, and H. Chang, “The applications of gold nanoparticle-initialed chemiluminescence in biomedical detection,†Springer New York LLC, Dec. 01, 2016,

https://doi.org/10.1186/s11671-016-1686-0

L. Liang et al., “Aptamer-based fluorescent and visual biosensor for multiplexed monitoring of cancer cells in microfluidic paper-based analytical devices,†Sens Actuators B Chem, 229, 347–354, Jun. 2016,

https://doi.org/10.1016/j.snb.2016.01.137

C. Li, Y. Liu, X. Zhou, and Y. Wang, “A paper-based SERS assay for sensitive duplex cytokine detection towards the atherosclerosis-associated disease diagnosis,†J Mater Chem B, 8(16), 3582–3589, Apr. 2020,

https://doi.org/10.1039/C9TB02469G

M. Rahbar, A. R. Wheeler, B. Paull, and M. Macka, “Ion-exchange based immobilization of chromogenic reagents on microfluidic paper analytical devices,†Anal Chem, 91(14), 8756–8761, Jul. 2019,

https://doi.org/10.1021/acs.analchem.9b01288

Y. Zhang et al., “Distance-based detection of Ag+ with gold nanoparticles-coated microfluidic paper,†J Anal Test, 5(1), 11–18, Mar. 2021,

https://doi.org/10.1007/s41664-021-00157-0

K. K. Bharadwaj et al., “Green synthesis of gold nanoparticles using plant extracts as beneficial prospect for cancer theranostics,†Molecules, 26(6389), 1–41, Nov. 2021,

https://doi.org/10.3390/molecules26216389

T. Medina, S. Erlangga, A. Bayu, D. Nandiyanto, and M. Fiandini, “Analisis tekno-ekonomi pada produksi nanopartikel emas (aunp) dengan metode biosintesis menggunakan sargassum horneri pada skala industri,†Jurnal Teknik Industri (JURTI), 1(2), 103–110, 2022,

https://doi.org/10.30659/jurti.1.2.103-110

F. Y. Kong, J. W. Zhang, R. F. Li, Z. X. Wang, W. J. Wang, and W. Wang, “Unique roles of gold nanoparticles in drug delivery, targeting and imaging applications,†Molecules, 22(1445), 1–13, Sep. 2017,

https://doi.org/10.3390/molecules22091445

E. Ferrari, “Gold nanoparticle-based plasmonic biosensors,†Biosensors (Basel), 13(411), 1–16, Mar. 2023,

https://doi.org/10.3390/bios13030411

J. B. Vines, J.-H. Yoon, N.-E. Ryu, D.-J. Lim, and H. Park, “Gold nanoparticles for photothermal cancer therapy,†Front Chem, 7(167), 1–16, 2019,

https://doi.org/10.3389/fchem.2019.00167

S. Amaliyah, D. P. Pangesti, M. Masruri, A. Sabarudin, and S. B. Sumitro, “Green synthesis and characterization of copper nanoparticles using piper retrofractum vahl extract as bioreductor and capping agent,†Heliyon, 6(e0636), 1–12, Aug. 2020,

https://doi.org/10.1016/j.heliyon.2020.e04636

G. A. Isola, M. K. Akinloye, Y. K. Sanusi, P. S. Ayanlola, and G. A. Alamu, “Optimizing x-ray imaging using plant mediated gold nanoparticles as contrast agent: A review,†International Journal of Research and Scientific Innovation, 08(08), 169–175, 2021,

https://doi.org/10.51244/IJRSI.2021.8809

S. Suvarna et al., “Synthesis of a novel glucose capped gold nanoparticle as a better theranostic candidate,†PLoS One, 12(6), 1–15, Jun. 2017,

https://doi.org/10.1371/journal.pone.0178202

N. J. Lang, B. Liu, and J. Liu, “Characterization of glucose oxidation by gold nanoparticles using nanoceria,†J Colloid Interface Sci, 428, 78–83, Aug. 2014,

https://doi.org/10.1016/j.jcis.2014.04.025

W. Agudelo, Y. Montoya, and J. Bustamante, “Using a non-reducing sugar in the green synthesis of gold and silver nanoparticles by the chemical reduction method,†DYNA (Colombia), 85(206), 69–78, Jul. 2018,

https://doi.org/10.15446/dyna.v85n206.72136

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Published

2024-08-01

Issue

Vol. 11 No. 1 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan

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Articles

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