RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA

Authors

  • Winni Astuti 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, Indonesia, Indonesia
  • Rabiatul Adawiyah 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, Indonesia, Indonesia
  • Amanda Aulia Putri 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, Indonesia, Indonesia
  • Djihan Ryn Pratiwi 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, Indonesia, Indonesia

DOI:

https://doi.org/10.15575/ak.v11i2.39438

Keywords:

Raw starch degrading amylase (RSDA), Karang Mumus River, Dinitrosalicylic Acid, Klebsiella pneumoniae

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.

References

Junejo, S.A.; Flanagan, B.M.; Zhang, B.; Dhital, S. “Starch structure and nutritional functionality–past revelations and future

prospectsâ€, Carbohyd. Polym., 277, 18837 2022, https://doi.org/10.1016/j.carbpol.2021.118837

Božić, NataÅ¡a, LonÄar, Nikola, Slavić, Marinela Å okarda and VujÄić, Zoran. "Raw starch degrading α-amylases: an unsolved riddle" Amylase, 1(1), pp. 12-25. 2017 https://doi.org/10.1515/amylase-2017-0002

Sun H, Zhao P, Ge X, Xia Y, Hao Z, Liu J, Peng M. “Recent advances in microbial raw starch degrading enzymesâ€. Applied Biochemistry and Biotechnology,160(4):988-1003, 2010,

https://doi.org/10.1007/s12010-009-8579-y

Li H., Dongbang Y, Yan P, Xin C, Fang Z and Yazhong X, “Enhanced extracellular raw starch-degrading α-amylase production in Bacillus subtilis by promoter engineering and translation initiation efficiency optimizationâ€, Microbial Cell Factories 21:127, 2022, https://doi.org/10.1186/s12934-022-01855-9

Shofiyah S.S., Yuliani ., Widya N., Sarian F. D., Puspasari F, Radjasa O.K., Ihsanawati, Natalia D., “Isolation, expression, and characterization of raw starch degrading α-amylase from a marine lake Bacillus megaterium NL3†Heliyon, 6(12), 2020 https://doi.org/10.1016/j.heliyon.2020.e0579

F.K Frima, R.B. Satiyarti, Y Anggraini, E Syafitri,I. A. Rini, “Characteristics of Raw-Starch Degrading Amylase Bacteria from Natar Hot Spring Lampung†Jurnal Kimia Sains dan Aplikasi, 23(7): 238-243, 2020, https://doi.org/10.14710/jksa.23.7.238-243

D.Prongjit, H Lekakarn, B Bunterngsook, K Aiewviriyasakul, W Sritusnee and V Champreda, “Functional Characterization of Recombinant Raw Starch Degrading α-Amylase from Roseateles terrae HL11 and Its Application on Cassava Pulp Saccharification†Catalysts, 12, 647-666, 2022 https://doi.org/10.3390/catal12060647

Wackett, L.P., “Microbial industrial enzymes: An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnologyâ€, Microb. Biotechnol., 12, 1090–1091, 2019, https://doi.org/10.1111/1751-7915.13389

Hamid B, Bashir Z, Yatoo AM, Mohiddin F, Majeed N, Bansal M, Poczai P, Almalki WH, Sayyed RZ, Shati AA, Alfaifi MY, “Cold-Active Enzymes and Their Potential Industrial Applications-A Reviewâ€, Molecules, Sep 10;27(18):5885., 2022, https://doi.org/10.3390%2Fmolecules27185885

Andi D. dan Syamsir, “Studi Kualitas Air Sungai Karang Mumus Ditinjau dari Parameter Escherichia coli Untuk Keperluan Higiene Sanitasiâ€, Jurnal Kesehatan Lingkungan Indonesia, 20(1), 2021, https://doi.org/10.14710/jkli.20.1.1-6

G.L. Miller, “Use of dinitro salicylic acid reagent for determination of reducing sugarâ€, Anal. Chem, 31, 426–428, 1959 https://doi.org/10.1021/ac60147a030

Novo, M, Casanoves, M, Garcia-Vallve, S., Pujadas, G., Mulero, M., Valls, C., “How do Detergents Work? A Qualitative Assay to Measure Amylase Activity†Journal of biological education, 2015,

http://dx.doi.org/10.1080/00219266.2015.1058843

Jay Kant Yadav & V. Prakash, “Stabilization of α-Amylase, the Key

Enzyme in Carbohydrates Properties Alterations, at Low pHâ€, International Journal of Food

Properties, 14(6), 1182-1196, 2011, https://doi.org/10.1080/10942911003592795

Yang G, Yao H, Mozzicafreddo M,

Ballarini P, Pucciarelli S, Miceli C. “Rational engineering of a cold-adapted α-amylase from the Antarctic ciliate Euplotes focardii for simultaneous improvement of thermostability

and catalytic activityâ€. Appl Environ Microbiol., 83:e00449-17, 2017, https://doi.org/10.1128/AEM.00449-17

Ume-e-Salma L, Shumaila N, Hafiz I H, “Optimization and production of highly stable amylase isolated from Citrobacter portucalensis using low cost agro industrial wastes as substrates: Prospective therapeuticsâ€, Kuwait Journal of Science, Volume 51, Issue 1, 2024 https://doi.org/10.1016/j.kjs.2023.11.005

Fang, W., Xue, S., Deng, P., Zhang, X., Wang, X., Xiao, Y., Fang, Z., “AmyZ1: A novel α-amylase from marine bacterium Pontibacillus sp. ZY with high activity toward raw starchesâ€, Biotechnology for Biofuels, 12(1), 2019 https://doi.org/10.1186/s13068-019-1432-9

F. Puspasari, O.K. Radjasa, a.S. Noer, Z. Nurachman, Y.M. Syah, M. van der Maarel, L. Dijkhuizen, S. Janecek, D. Natalia, “Raw starch-degrading α- amylase from Bacillus aquimaris MKSC 6.2: isolation and expression of the gene, bioinformatics and biochemical characterization of the recombinant enzymeâ€, J. Appl. Microbiol. 114, 108–120, 2013, https://doi.org/10.1111/jam.12025

Vidilaseris, K., Hidayat, K., Retnoningrum, D.S. et al., “Biochemical characterization of a raw starch degrading α-amylase from the Indonesian marine bacterium Bacillus sp. ALSHL3â€. Biologia, 64, 1047–1052 2009. https://doi.org/10.2478/s11756-009-0190-8

Z. Nurachman, A. Kono, O. K. Radjasa, D. Natalia, “Identification a novel raw-starch-degrading-α-amylase from a tropical marine bacteriumâ€, American Journal of Biochemistry and Biotechnology, 6(4), 300-306, 2010, https://doi.org/10.3844/ajbbsp.2010.300.306

Downloads

Published

2024-12-30

Issue

Vol. 11 No. 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan

Section

Articles

Citation Check

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).