Effect of Copper-Based Fungicide on Chemical Composition of Cocoa Seeds
DOI:
https://doi.org/10.15575/ak.v12i1.43696Keywords:
chemical composition, cocoa, copper-based fungicide, proximate analysisAbstract
Production of cocoa seeds, one of Nigeria’s major non-oil foreign exchange earners as well as a major raw material for the beverage industry, is greatly hindered by diseases caused by various species of the genus Phytophthora. To avert this, copper-based fungicides are sprayed on the leaves of cocoa trees to control or prevent the survival of this organism by the farmers without paying attention to the effects of this chemical on the proximate composition of cocoa seeds. This study therefore investigated the effects of a copper-based fungicide (Ridomil Gold Copper) on the quality of the cocoa seeds by spraying cocoa trees, including the pods, with 50.00 g/L of copper-based fungicide. The results obtained showed that cocoa seeds from the control trees showed significantly higher contents of fiber (4.51%), protein (15.1%), and fat (36.1%) when compared to the respective values of 3.45, 3.95, and 7.59% obtained for the cocoa seeds harvested from the fungicide-treated cocoa trees. All other proximate compositions did not show any statistical difference, except for carbohydrate and calorific values, which were significantly higher in cocoa seeds from fungicide-treated cocoa trees. Seeds from fungicide-treated trees showed significantly higher potassium, phosphorus, and magnesium contents but lower zinc and copper contents. However, phytochemicals such as phenols, alkaloids, flavonoids, and tannins were significantly lower in content in the seeds of fungicide-treated cocoa trees. The contents of glycosides and antioxidants in the cocoa seeds were statistically similar for both the control and treatment, except for ascorbic acid, which showed a significantly lower value (4.8 mg/100 ml) in cocoa seeds from fungicide-treated cocoa trees, compared with the value recorded for the control in this study (13.33%). The foregoing results showed that the use of copper-based fungicides for the control of black pod disease in cocoa adversely affected the quality of cocoa seeds from the treated trees.
References
[1] I. I. O. Abu, Z. Szantoi, A. Brink, M. Robuchon, and M. Thiel, "Detecting cocoa plantations in Côte d’Ivoire and Ghana and their implications on protected areas", Ecological Indicators, 129, 107863, 2021, https://doi.org/10.1016/j.ecolind.2021.107863
[2] O. Boysen, E. Ferrari, V. Nechifor, and P. Tillie, "Earn a living? What the Côte d’Ivoire–Ghana cocoa living income differential might deliver on its promise", Food Policy, 114, 102389, 2023, https://doi.org/10.1016/j.foodpol.2022.102389
[3] O. O. Bukola, E. Akinfisoye, and F. Abimbola, "Effects of climate variability on cocoa production in Ondo State, Nigeria", American Journal of Climate Change, 10(4), 396–406, 2021, https://doi.org/10.4236/ajcc.2021.104020
[4] N. M. Abdullahi, S. Shahriar, S. Kea, A. M. Abdullahi, Q. Zhang, and X. Huo, "Nigeria’s cocoa exports: a gravity model approach", Ciência Rural, 51(11), 20201043, 2021, https://doi.org/10.1590/0103-8478cr20201043
[5] A. Adeniyi, "Diversity of cacao pathogens and impact on yield and global production", in Theobroma cacao – Deploying Science for Global Economy, P. O. Aipokpodion, Ed. London: IntechOpen, 2019. https://doi.org/10.5772/intechopen.73761
[6] J. Y. Opoku, A. Y. Akrofi, and A. A. Appiah, "The spread of Phytophthora megakarya on cocoa in Ghana", Journal of the Ghana Science Association, 2(3), 110–116, 2004, https://doi.org/10.4314/jgsa.v2i3.18000
[7] L. Tamm, B. Thuerig, S. Apostolov, H. Blogg, E. Borgo, P. E. Corneo, S. Fittje, M. de Palma, A. Donko, C. Experton, É. A. Marín, Á. M. Pérez, I. Pertot, A. Rasmussen, H. Steinshamn, A. Vetemaa, H. Willer, and J. Herforth-Rahmé, “Use of copper-based fungicides in organic agriculture in twelve European countries”, Agronomy, 12(3), 673 2022, https://doi.org/10.3390/agronomy12030673
[8] V. E. Manga, B. N. Fru, and G. Y. Sendze, "Heavy metal soil contamination in cocoa plantations in South West region, Cameroon", Journal of Ecology and Natural Environment, 12(3), 95–103, 2020, https://doi.org/10.5897/JENE2020.0828
[9] N. A. Kulikova and I. V. Perminova, "Interactions between humic substances and microorganisms and their implications for nature-like bioremediation technologies", Molecules, 26(9), 2706, 2021, https://doi.org/10.3390/molecules26092706
[10] R. A. Aziz, M. Yiwen, M. Saleh, M. N. Salleh, S. C. B. Gopinath, S. G. E. Giap, S. V. Chinni, and R. Gobinath, “Bioaccumulation and translocation of heavy metals in paddy (Oryza sativa L.) and soil in different land use practices”, Sustainability, 15(18), 13426, 2023, https://doi.org/10.3390/su151813426
[11] Q. Zhu, J. Ji, X. Tang, C. Wang, and H. Sun, "Bioavailability assessment of heavy metals and organic pollutants in water and soil using DGT: A review", Applied Science, 13(17), 9760, 2023, https://doi.org/10.3390/app13179760
[12] A. Alengebawy, S. T. Abdelkhalek, S. R. Qureshi, and M. Q. Wang, "Heavy metals and pesticides toxicity in agricultural soil and plants: ecological risks and human health implications", Toxics, 9(3), 42, 2021, https://doi.org/10.3390/toxics9030042
[13] J. Wade, M. Ac-Pangan, V. R. Favoretto, A. J. Taylor, N. Engeseth, and A. J. Margenot, “Drivers of cadmium accumulation in Theobroma cacao L. beans: A quantitative synthesis of soil-plant relationships across the Cacao Belt”, PLOS One, 17(2), e0261989, 2022, https://doi.org/10.1371/journal.pone.0261989
[14] F. A. Sowunmi, G. T. Famuyiwa, K. A. Oluyole, S. O. Aroyeun, and O. A. Obasoro, "Environmental burden of fungicide application among cocoa farmers in Ondo state, Nigeria", Scientific African, 6, e00207, 2019. https://doi.org/10.1016/j.sciaf.2019.e00207
[15] AOAC, Official Methods of Analysis, 18th ed. Gaithersburg, MD, USA: Association of Official Analytical Chemists, 2005.
[16] AOAC, Official Methods of Analysis, 17th ed. Washington, DC: Association of Official Analytical Chemists, 2000. Methods 935.14 and 992.24.
[17] S. O. Oyedemi, G. Bradley, and A. J. Afolayan, "In-vitro and in-vivo antioxidant activities of aqueous extract of Strychonos henningsii Gilg", African Journal of Pharmacy and Pharmacology, 4(2), 70–78, 2010, Available online http://www.academicjournals.org/ajpp
[18] M. Djali, K. Santasa, R. Indiarto, E. Subroto, F. Fetriyuna, and E. Lembong, "Proximate composition and bioactive compounds of cocoa bean shells as a by-product from cocoa industries in Indonesia", Foods, 12(17), 3316, 2023, https://doi.org/10.3390/foods12173316
[19] E. I. Adeyeye, "Proximate, Mineral, and Antinutrient Compositions Cocoa cake, cocoa liquor and alkalized cocoa powders sourced in Nigeria", Journal of Advanced Pharmaceutical Science and Technology, 1(3), 12–28, 2013, http://dx.doi.org/10.14302/issn.2328-0182.japst-15-855
[20] E. O. Afoakwa, J. Quao, J. Takrama, A. S. Budu, and F. K. Saalia, "Chemical composition and physical quality characteristics of Ghanaian cocoa seeds as affected by pulp pre-conditioning and fermentation", Journal of Food Science and Technology, 50(6), 1097–1105, 2013, https://doi.org/10.1007/s13197-011-0446-5
[21] M. H. Assareh, A. Shariat, and A. Ghamari-Zare, "Seedling response of three Eucalyptus species to copper and zinc toxic concentrations", Caspian Journal of Environmental Science, 6(2), 97–103, 2018, Online version is available on http://research.guilan.ac.ir/cjes
[22] A. Assa, D. W. Asriati, D. Indriana, and A. L. Sampebarra, "Mineral contents in fermented bean shells of Forastero-cocoa (Theobroma cacao L.) clones", IOP Confrences Series: Earth and Environmental Science, 355, 012108, 2019, https://doi.org/10.1088/1755-1315/355/1/012108
[23] C. E. Duru, "Mineral and phytochemical evaluation of Zea mays husk", Scientific African, 7, e00224, 2020, https://doi.org/10.1016/j.sciaf.2019.e00224
[24] S. D. Upadhaya and I. H. Kim, "Importance of micronutrients in bone health of monogastric animals and techniques to improve the bioavailability of micronutrient supplements - A review", Asian-Australasian Journal of Animal Science, 33(12), 1885–1895, 2020, https://doi.org/10.5713/ajas.19.0945
[25] A. Kumar, P. Nirmal, M. Kumar, A. Jose, V. Tomer, E. Oz, C. Proestos, M. Zeng, T. Elobeid, K. Sneha., and F. Oz, "Major phytochemicals: recent advances in health benefits and extraction method", Molecules, 28(2), 887, 2023, https://doi.org/10.3390/molecules28020887
[26] P. Kumah, S. O. Ampofo, and I. A. Idun, "Phytochemical screening of cocoa (Theobroma cacao L.) leaves", Acta Horticultura, 1364, 459–466, 2023, https://doi.org/10.17660/ActaHortic.2023.1364.58
[27] I. L. Kayaputri, M. Djali, N. Sukri, and R. H. Fazaryasti, "The antimicrobial effectiveness of cacao shell and cacao husk combination on inhibition of pathogenic bacteria in food products", IOP Confrence Series: Earth and Environmental Science, 443, 012077, 2020, https://doi.org/10.1088/1755-1315/443/1/012077
[28] N. Bechara, V. M. Flood, and J. E. Gunton, "A systematic review on the role of vitamin C in tissue healing", Antioxidants (Basel), 11(8), 1605, 2022, https://doi.org/10.3390/antiox11081605
[29] E. Nascimento da Silva, D. da Cruz. Ramos, L. M. Menezes, A. O. de Souza, S. C. da Silva Lannes, M. V. da Silva, "Nutritional value and antioxidant capacity of ‘cocoa honey’ (Theobroma cacao L.)", Food Science and Technology, 34(4), 755–759, 2014, https://doi.org/10.1590/1678-457X.6447
[30] P. E. Aikpokpodion, L. Lajide, and A. F. Aiyesanmi, "Heavy metals contamination in fungicide treated cocoa plantations in Cross River State, Nigeria", American-Eurasian Journal of Agriculture and Environmental Science, 8(3), ISSN 1818-6769, 268–274, 2010.
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