Aktivitas Penghambatan α-Glukosidase Campuran Ekstrak Daun Namnam (Cynometra cauliflora L.) dan Madu Kaliandra


La Ode Sumarlin(1*), Dede Sukandar(2), Lia Pratiwi(3)

(1) Jurusan Kimia Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta, Indonesia
(2) Jurusan Kimia Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta, Indonesia
(3) Jurusan Kimia Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta, Indonesia
(*) Corresponding Author

Abstract


Penyakit diabetes melitus adalah disfungsi metabolisme karbohidrat. Terapi antidiabetes oral yaitu menggunakan agen penghambat α-glukosidase. Oleh karena itu perlu dilakukan kajian agen penghambat α-glukosidase yang potensial diantaranya madu kaliandra dan ekstrak daun namnam.  Kajian ini akan difokuskan pada kemampuan sampel tersebut sebagai agen penghambat α-glukosidase baik dengan perlakuan bentuk tunggal maupun campuran, sebelum dan setelah fraksinasi. Selain itu digunakan FTIR (Fourier Transform Infrared) untuk mengetahui golongan senyawa pada setiap perlakuan sampel tersebut. Uji aktivitas penghambatan α-glukosidase secara in vitro pada ekstrak daun namnam menunjukkan nilai IC50 sebesar 34,47 ppm, sedangkan madu kaliandra tidak menunjukkan aktivitas penghambatan α-glukosidase. Hasil fraksinasi cair-cair menunjukkan bahwa ekstrak n-heksana mampu menghambat α-glukosidase tertinggi berdasarkan nilai IC50 sebesar 8,46 ppm. Hasil karakterisasi menggunakan spektrofotometer FTIR pada ekstrak n-heksana mengandung gugus fungsi yang memiliki kemiripan dengan golongan senyawa steroid. Senyawa aktif pada ekstrak etil asetat dan ekstrak n-butanol mengandung gugus fungsi yang memiliki kemiripan dengan gugus fungsi standar senyawa kuersetin.


Keywords


Biochemistry; Organic Chemistry

Full Text:

PDF

References


Brunner and Suddarth, Buku Ajar Keperawatan Medikal Bedah. Edisi 8, Jakarta: Penerbit Buku Kedokteran EGC, 2002.

A. Aziz and Iqbal, M, “Antioxidant activity and phytochemical composition of Cynometra cauliflora,” Journal of Experimental and Integrative Medicine, vol. 3, no. 4, pp. 337-341, 2013.

M.A., Ado, F., Abas, Ismail., H., Ghazali, and K, Shaari, “Chemical Profile and Anticetylcholinesterase, Antityrosinase, Antioxidant, α-Glucosidase Inhibitory Activity of Cynometra cauliflora L. Leaves,” Journal of the Science of Food and Agriculture, vol. 95, no. 3, pp. 635-42, 2014.

L, Sumarlin, A, Suprayogi, M, Rahminiwati, A, Satyaningtijas, D, Sukandar, and H, Pangestika, “Ability of leaf extract namnam (Cynometra cauliflora) as antidiabetic agents α -glucosidase inhibition through at some stage extraction,” International Journal of Sciences: Basic and Applied Research, vol. 30, no. 2, pp. 12-123, 2016.

A, Kadirvelu, “Potential benefits of honey in type 2 diabetes mellitus: A Review,” International Journal of Collaborative Research on Internal Medicine & Public Health, vol. 5, no. 4, pp. 199, 2013

D, Sofawati, “Uji Aktivitas Antidiabetes Fraksi-Fraksi Buah Ketapang (Terminalia catappa L.) Dengan Metode Penghambatan Aktivitas α-glukosidase dan Identifikasi Golongan Senyawa Kimia Dari Fraksi Yang Aktif,” Skripsi, Farmasi. Universitas Indonesia.

Y.M., Kim, M.H. Wang, and H.I. Rhee, “A novel α-glucosidase inhibitor from pine bark,” Carbohydrate Research, vol. 339, no. 3, pp. 715-717, 2004.

S., Sugiawati, S., Setiasih, dan E., Afifah, “Antyhyperglicemic Activity of Mahkota Dewa [Phaleria macrocarpa (Scheff.) Boerl.] Leaf Extracts As An Alpha Glucosidase Inhibitor,” Makara Kesehatan, vol. 13, no. 2, pp. 74-78, 2009

S-H., Jo, E-H., Ka, H-S.Lee, E., Apostolidis, H-D., Jang, and Y-I., Kwon, “Comparison of antioxidant potential and rat intestinal α-Glucosidases inhibitory activities of kuersetin, rutin, and isokuersetin,” International Journal of Applied Research in Natural Products, vol. 2, no.4, pp. 52- 60, 2009.

J., Shinde, T., Taldone, M., Barletta, N., Kunaparaju, B., Hu, S., Kumar, J., Placido, and S.W., Zito, “Alpha-Glucosidase inhibitory activity of Syzygium cumini (Linn.) skeels seed kernel in vitro and in goto–kakizaki (GK) rats,” Carbohydrate Research, vol. 343, no. 7, pp. 1278–1281, 2008.

P.C., Molan, “The potential of honey promote oral wellness,” General Dentistry, vol. 49, no. 6, pp. 24-34. 2001.

V., Robson, S., Dodd, and S., Thomas, “Standarized antibacterial honey (Medihoney) with Standard Therapy in Wound Care: Randomized Clinical Trial,” Journal of Advamced Nursing, vol. 65, no. 3, pp. 565-75, 2008.

S. Bogdanov, “Honey as Nutrient and Functional Food: A review,” Bee Product Science, vol. 3, no. 2, pp.1-31, 2011.

J.B. Harborne, Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan edisi kedua, ITB: Bandung, 1996.

P. Meir and Z. Yaniv, “An in vitro study on the effect of Momordica charantia on glucose uptake and glucose metabolism in rats”, Planta Medica, vol. 51, no. 1, pp. 12-16, 1985.

J. Welihinda and E.H. Karunanayake, “Extra-pancreatic effects of Momordica charantia in rats,” Journal of Ethnopharmacology, vol. 17, no. 3, pp.247-255, 1986.

D.S. Lee and S.H Lee, “Genestein, a Soy Isoflavone is A Poten α-Glucosidase Inhibitors”. FEBS Letters, vol. 501, no. 1, pp.84-86, 2001.

J.S. Kim and C.S.S.K.H. Kwon, “Inhibition of Alpha-glucosidase and Amylase by Luteolin, a Flavonoid,” Bioscience, Biotechnology, and Biochemistry, vol. 64, no. 11, pp.2458–2461. 2000.

D. Kalita, D.G. Holm, D.V. LaBarbera, J.M. Petrash, and S.S. Jayanty, “Inhibition of α-glucosidase, α-amylase, and aldose reductase by potato polyphenolic compounds”, PLoS ONE, vol. 13, no. 1, pp. e0191025, 2018.

G.J., McDougall, F., Shpiro, P., Dobson, P.,Smith, A., Blake, and D., Stewart,. “Different polyphenolic components of soft fruits inhibit α-amylase and α-glucosidase,” Journal of Agricultural and Food Chemistry, vol. 53, no. 7, pp.2760-2766, 2005.

A.,Schäfer, and P., Hőgger, “Oligomeric procyanidins of French maritime pine bark extract (Pycnogenol®) effectively inhibits α-glucosidase,” Diabetes Research and Clinical Practice, vol. 77, no. 1, pp.4146, 2007.

L., Zhang, Z.C., Tu, T., Yuan, H., Wang, X., Xie, and Z.F., Fu,. “Antioxidants and α-glucosidase inhibitors from Ipomoea batatas leaves identified by bioassay-guided approach and structure-activity relationships,” Food Chemistry vol. . 208, pp.61-67, 2016

X., Zhou, J., Liang, Y., Zhang, H., Zhao, Y., Guo, and S., Shi, “Separation and purification of α-glucosidase inhibitors from Polygonatum odoratum by stepwise high-speed counter-current chromatography combined with Sephadex LH-20 chromatography target-guided by ultrafiltration–HPLC screening,” Journal of Chromatography B, vol. 985, no.15, pp.149-154, 2015.

M., Glisckman, “Food Hydrocolloid,” Vol-III. Baco Raton, FI. CRP Press, New York. 1986.

M.K., Unnikrishnan, V., Veerapur, Y., Nayak, P.P., Mudgal, and G., Mathew, “Antidiabetic, Antihyperlipidemic and Antioxidant Effects of the Flavonoids,” Polyphenols in Human Health and Disease, vol. 1, pp. 143-161, 2014.




DOI: https://doi.org/10.15575/ak.v6i2.6577

Copyright (c) 2020 La Ode Sumarlin, Dede Sukandar, Lia Pratiwi

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

CrossrefSINTAGoogle ScholarIndonesia One Search

View My Stats

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.