Hydropriming Improves Germination and Plant Recovery During Embryo Rescue of Wild Banana Musa acuminata var. tomentosa


Apriliana Dyah Prawestri(1*), Indira Riastiwi(2), Resa Sri Rahayu(3), Tri Handayani(4), Aryani Leksonowati(5), Yuyu Suryasari Poerba(6)

(1) Research Center for Biology, Indonesian Institute of Sciences (LIPI); Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, West Java, 16911, phone: (+62)21-8765058, fax: (+62)21-87907612, Indonesia
(2) Research Center for Biology, Indonesian Institute of Sciences (LIPI); Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, West Java, 16911, phone: (+62)21-8765058, fax: (+62)21-87907612, Indonesia
(3) Research Center for Biology, Indonesian Institute of Sciences (LIPI); Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, West Java, 16911, phone: (+62)21-8765058, fax: (+62)21-87907612, Indonesia
(4) Research Center for Biology, Indonesian Institute of Sciences (LIPI); Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, West Java, 16911, phone: (+62)21-8765058, fax: (+62)21-87907612, Indonesia
(5) Research Center for Biology, Indonesian Institute of Sciences (LIPI); Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, West Java, 16911, phone: (+62)21-8765058, fax: (+62)21-87907612, Indonesia
(6) Research Center for Biology, Indonesian Institute of Sciences (LIPI); Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, West Java, 16911, phone: (+62)21-8765058, fax: (+62)21-87907612, Indonesia
(*) Corresponding Author

Abstract


Wild bananas are believed to have genes for resistance to biotic and abiotic stress in nature, making them potential genetic resources for creating superior varieties. Wild banana seeds, such as Musa acuminata var. tomentosa are generally difficult to germinate in vivo, so that in vitro embryo culture technique is needed. This study aimed to increase embryo germination and regeneration of wild banana M. acuminata var. tomentosa by soaking the seeds as hydropriming. The treatment comprised of soaking the seeds in sterile distilled water for four periods of time: 0 (control), 1, 4, and 7 days. A total of 45 embryos for each treatment were planted on petri dishes containing MS + 0.5 mg/L BA + 1 mg/L biotin + 1 mg/L proline. The results showed that hydropriming increased the rate of embryo germination and regeneration. Seeds soaked for 1, 4, and 7 days successfully resulted in embryo germination percentages of 87%, 62%, and 62%, respectively, while the control unsoaked seeds germinated with a lower percentage of 42%. One-day soaking treatment was the most optimal treatment to increase the rate of germination and regeneration as well as obtained the best vigor as demonstrated by the highest average height of plantlets, number of leaves, and roots than other treatments. Thus, 1-day seed hydropriming is the best treatment for embryo rescue and regeneration of wild banana M. acuminata var. tomentosa


Keywords


Embryo rescue, germination, regeneration, seeds soaking, wild banana

Full Text:

PDF

References


Arun, K., Uma, S., Saraswathi, M. S., Backiyarani, S., & Durai, P. (2013). Effects of whole seed priming on the in vitro germination of hybrid banana embryos (Musa spp.). Seed Science and Technology, 41(3), 439–451.

Asif, M. J., Mak, C., & Othman, R. Y. (2001). In vitro zygotic embryo culture of wild Musa acuminata ssp. malaccensis and factors affecting germination and seedling growth. Plant Cell, Tissue and Organ Culture, 67(3), 267–270.

Bakry, F. (2008). Banana protocol zygotic embryo rescue in bananas. Fruits, 63(2), 111–115.

Bohra, P., Waman, A. A., & Jerard, B. A. (2020). Seed germination and storage studies in seed fertile Musa indandamanensis and its conservation. South African Journal of Botany, 128(2020), 161–166.

Burgos-Hernández, M., Castillo-Campos, G., Mata-Rosas, M., González, D., Vovides, A. P., & Murguía-González, J. (2014). Seed germination of the wild banana Musa ornata (Musaceae). Seed Science and Technology, 42(1), 16–27.

Christelová, P., De Langhe, E., Hřibová, E., Čížková, J., Sardos, J., Hušáková, M., Van den houwe, I., Sutanto, A., Kepler, A. K., Swennen, R., Roux, N., & Doležel, J. (2017). Molecular and cytological characterization of the global Musa germplasm collection provides insights into the treasure of banana diversity. Biodiversity and Conservation, 26(4), 801–824.

Cortleven, A., & Schmülling, T. (2015). Regulation of chloroplast development and function by cytokinin. Journal of Experimental Botany, 66(16), 4999–5013.

Dayarani, M., Dhanarajan, M. S., Arun, K., Uma, S., & Narayani, P. (2014). Embryo culture and embryo rescue studies in wild Musa spp. (Musa ornata). Journal of Applied Horticulture, 16(2), 126–130.

Diro, M., & Van Staden, J. (2003). In vitro regeneration of Ensete ventricosum from zygotic embryos of stored seeds. South African Journal of Botany, 69(3), 364–369.

Esmaeilpour, A., & Van Damme, P. (2016). Evaluation of seed soaking times on germination percentage, germination rate and growth characteristics of pistachio seedlings. Acta Horticulturae, 1109, 107–112.

Frank, A., Saldierna Guzmán, J., & Shay, J. (2017). Transmission of bacterial endophytes. Microorganisms, 5(4), 70.

Hoang, N. H., Kane, M. E., Radcliffe, E. N., Zettler, L. W., & Richardson, L. W. (2017). Comparative seed germination and seedling development of the ghost orchid, Dendrophylax lindenii (Orchidaceae), and molecular identification of its mycorrhizal fungus from South Florida. Annals of Botany, 119(3), 379–393.

Kallow, S., Longin, K., Sleziak, N. F., Janssens, S. B., Vandelook, F., Dickie, J., Swennen, R., Paofa, J., Carpentier, S., & Panis, B. (2020). Challenges for ex situ conservation of wild bananas: Seeds collected in Papua New Guinea have variable levels of desiccation tolerance. Plants, 9(9), 1–21.

Mozumder, S. N., & Hossain, M. M. (2013). Effect of seed treatment and soaking duration on germination of Eryngium foetidum L. seeds. International Journal of Horticulture, 3(10), 46–51.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.

Oliveira, C. E. D. S., Steiner, F., Zuffo, A. M., Zoz, T., Alves, C. Z., & De Aguiar, V. C. B. (2019). Seed priming improves the germination and growth rate of melon seedlings under saline stress. Ciencia Rural, 49(7), 1–11.

Pawar, V. A., & Laware, S. L. (2018). Seed priming: A critical review. International Journal of Scientific Research in Biological Sciences, 5(5), 94–101.

Roostika, I., Sutanto, A., Edison, N., & Dewi, N. (2019). Kultur embrio pisang liar Musa acuminata ssp. sumatrana yang langka. Jurnal Hortikultura, 28(1), 25.

Sabongari, S., & Aliero, B. L. (2004). Effects of soaking duration on germination and seedling growth of tomato (Lycopersicum esculentum Mill). African Journal of Biotechnology, 3(1), 47–51.

Santos, A. S., Amorim, E. P., Ferreira, C. F., & Pirovani, C. P. (2018). Water stress in Musa spp.: A systematic review. PLoS ONE, 13(12), 1–17.

Shah, H., Jalwat, T., Arif, M., & Miraj, G. (2012). Seed priming improves early seedling growth and nutrient uptake in mungbean. Journal of Plant Nutrition, 35(6), 805–816.

Siddique, A., & Kumar, P. (2018). Physiological and biochemical basis of pre-sowing soaking seed treatment - An overview. Plant Archives, 18(2), 1933–1937.

Silva, M. D. S., Rodrigues, T. C., Aud, F. F., Dos Santos-Serejo, J. A., & Amorim, E. P. (2019). Illustrated guide to the classification of banana seeds and embryos. Revista Brasileira de Fruticultura, 41(2), 1–5.

Singh, S., Thangjam, R., Harish, G. D., Singh, H., Kumar, R., Meena, D. P. S., & Agrawal, A. (2020). Conservation protocols for Ensete glaucum, a crop wild relative of banana, using plant tissue culture and cryopreservation techniques on seeds and zygotic embryos. Plant Cell, Tissue and Organ Culture, 144, 195–209.

Sousa, K. C. I., De Araújo, L. G., De Sousa Silva, C., De Carvalho, J. C. B., Sibov, S. T., De Almeida Gonçalves, L., Pereira, M. C., Gonçalves, F. J., & Da Corsi De Filippi, M. C. (2019). Seed germination and development of orchid seedlings (Cyrtopodium saintlegerianum) with fungi. Rodriguesia, 70, 1–11.

Sulistyaningsih, L. D. (2013). Pisang-pisangan (Musaceae) di Gunung Watuwila dan daerah sekitarnya. Floribunda, 4(5), 121–125.

Tania, S. S., Hossain, M. M., & Hossain, M. A. (2019). Effects of hydropriming on seed germination, seedling growth and yield of bitter gourd. Journal of the Bangladesh Agricultural University, 17(3), 281–287.

Tenkouano, A., Vuylsteke, D., Okoro, J., Makumbi, D., Swennen, R., & Ortiz, R. (2003). Diploid banana hybrids TMB2x5105-1 and TMB2x9128-3 with good combining ability, resistance to black Sigatoka and nematodes. HortScience, 38(3), 468–472.

Uma, S., Lakshmi, S., Saraswathi, M. S., Akbar, A., & Mustaffa, M. M. (2011). Embryo rescue and plant regeneration in banana (Musa spp.). Plant Cell, Tissue and Organ Culture, 105(1), 105–111.

Uma, S., Lakshmi, S., Saraswathi, M. S., Akbar, A., & Mustaffa, M. M. (2012). Plant regeneration through somatic embryogenesis from immature and mature zygotic embryos of Musa acuminata ssp. burmannica. In Vitro Cellular and Developmental Biology - Plant, 48(5), 539–545.

ur Rehman, H., Iqbal, H., Basra, S. M. A., Afzal, I., Farooq, M., Wakeel, A., & Wang, N. (2015). Seed priming improves early seedling vigor, growth and productivity of spring maize. Journal of Integrative Agriculture, 14(9), 1745–1754.

Wilson, V., & Tenkouano, A. (2019). Effects of storage and priming on seed emergence in soil and embryo culture of Musa acuminata Calcutta 4. Asian Plant Research Journal, 2(1), 1–9.

Wu, W., Yang, Y. L., He, W. M., Rouard, M., Li, W. M., Xu, M., Roux, N., & Ge, X. J. (2016). Whole genome sequencing of a banana wild relative Musa itinerans provides insights into lineage-specific diversification of the Musa genus. Scientific Reports, 6(31586), 1–11.

Zhang, C., Wu, J., Fu, D., Wang, L., Chen, J., Cai, C., & Ou, L. (2015). Soaking, temperature, and seed placement affect seed germination and seedling emergence of Litchi chinensis. HortScience, 50(4), 628–632.

Zhang, L., Cenci, A., Rouard, M., Zhang, D., Wang, Y., Tang, W., & Zheng, S. J. (2019). Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4. Scientific Reports, 9(1), 1–14.




DOI: https://doi.org/10.15575/biodjati.v6i1.10528

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Jurnal Biodjati



Indexing By :

      

      

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

View My Stats