Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.)


Farah Aldila(1*), Ari Susilowati(2), Ratna Setyaningsih(3)

(1) Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292, Indonesia
(2) Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292, Indonesia
(3) Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292, Indonesia
(*) Corresponding Author

Abstract


Polyurethane is a class of polymers characterized by their substantial tensile strength and high melting points which enables them to be extremely durable. Despite its xenobiotic origin, polyure-thane has been found susceptible to biodegradation by microorgan-isms. The main object of this research was to investigate polyurethane degradation by the bacterium isolated from decayed teak wood (Tec-tona grandis Linn. f.). Polyurethane degrading bacteria were isolated using selective medium in the form of LB (Luria Bertani) with the ad-dition of 0.3% Impranil. Bacterial biodegradation activities shown by the changes of polyurethane structure analyzed by FT-IR spectrosco-py. The bacterial identification was carried out based on observations on the morphological characteristics of the colonies, cell morphology and sequences of 16S rRNA encoding genes. One of the potential iso-late that successfully isolated was K9, which demonstrates the disap-pearance of the 1735/cm peak of the characteristic function urethane in the FT-IR analysis. Analysis of the 16S rRNA encoding gene showed that the potential isolate having 98% similarity index to Bacillus safen-sis strain FO-36b. The activity shown by the isolate suggests that the bacteria could be a promising agent for polyurethane degradation. 


Keywords


biodegradation; polyurethane; teak wood

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References


Akutsu, Y, Nakajima-Kambe T, Onuma F & Nakahara T. (1997). Determination of the Polyester Polyurethane Breakdown Products and Distribution of the Pol-yurethane Degrading Enzyme of Co-mamonas acidovorans strain TB-35. J. Ferment. Bioeng., 83, 456–460.

Ambriyanto, K. S. (2010). Isolasi dan Kar-akterisasi Bakteri Aerob Pendegradasi Selulosa dari Serasah Daun Rum-put Gajah (Pennisetum purpureum schaum). Skripsi. Institut Teknologi Sepuluh Nopember.

Bayer, O. (1947). Polyurethanes. Modern Plastics, 24, 149–152.

Bosshard, P.P., Abels, S., Zbinden, R., Ttger, E. C. B. & Altwegg, M. (2003). Riboso-mal DNA Sequencing for Identification of Aerobic Gram Positive Rods in the Clinical Labolatory (an 18 Month Eval-uation). Journal of Clinical Microbiolo-gy, 41(9), 4234 – 4140.

Carpita, N & McCann, M. (2000). The cell wall. Biochemistry and Molecular Biol-ogy of Plants. New Jersey: John Wiley and Sons Publishing.

Covestro. (2018). Dispersion. Germany: Covestro Deutschland AG.

Hermiati, E., Djumali, M., Sunarti, T. C., Su-parno, O. & Bambang, P. (2010). Peman-faatan Biomassa Lignoselulosa Ampas Tebu untuk Produksi Bioetanol. Jur-nal Litbang Pertanian, 29(4), 121-130.

Howard, G. T.(2002). Biodegradation of Pol-yurethane: a Review. Int. Biodeterior. Biodegrad, 49, 245–252.

Indrawati, I. & Rizki, A. F. M. (2017). Potensi Ekstrak Buah Buni (Antidesma bunius L) sebagai Antibakteri dengan Bakteri Uji Salmonella thypimurium dan Bacil-lus cereus. Jurnal Biodjati, 2(2), 138- 148.

Inspiring. (2018). Daerah Penghasil Kayu Jati Terbesar di Indonesia. Retrieved from https://inspiring.id/daerah-penghas-il-kayu-jati/.

Kristianingrum, S. (2015). Spektroskopi Infra Merah (Infrared Spectroscopy, IR). Yo-gyakarta: Pendidikan Kimia UNY.

Kurnia, K., Nina, H. S. & Syafitri, J.(2016). Isolation of Heterotrophic Bacteria from Cibuntu pond, West Java and charac-terization of metal and acid resistance. AL-KAUNIYAH Journal of Biology, 9(2), 74-79.

Marchesi, J. R., Sato, T., Weightman, A. J., Martin, T. A., Fry, J. C., Hion, S. J. & William G. W. (1998). Design and Evaluation of Useful Bacterium-Spe-cific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA. Ap-plied and Environmental Microbiology, 64(2), 795–799.

Nair, S & Kumar, P.(2007). Molecular charac-terization of a lipase-producing Bacil-lus pumilus strain (NMSN-1d) utilizing colloidal water-dispersible polyure-thane. World J Microbiol Biotechnol, 23, 1441–1449.

Nakkabi, A., Moulay, S., Saad I. & Moham-med, F. (2015c). Biological Degrada-toion of Polyurethane by Newly Isolated Microorganism. International Journal of Recent Advances in Multidisciplinary Research, 2(2), 222-225.

Nakkabi, A., Najim, I., Mohamed, D., En-carnación M. D. & Mohammed, F. (2015b). Growth of Pseudomonas in polyurethane medium. IOSR Journal of Engineering (IOSRJEN), 5, 36-40.

Nakkabi, A., Sadiki, M., Fahim, M., Ittobane, N., Ibnsouda Koraichi, S., Barkai, H. & El abed, S. (2015a). Biodegradation of Poly(ester urethane)s by Bacillus subti-lis. Int. J. Environ. Res., 9(1), 157-162.

Russell, J. R. Huang, J., Anand, Kucera, K., Amanda, G., Kathleen, W. D., DaShawn, Justin J., Farrah, M. K., David, K., Dan-iel, H. M., Paul, A. M., Salvador, J., Ma-rina, S., Maria, A., Michael V. Neely E. Williams Lori-Ann B. Carol B. & Scott A. S. (2011). Biodegradation of Polyes-ter Polyurethane by Endophytic Fungi. Applied and Environmental Microbiol-ogy, 77(17), 6076-6084.

Sagita, D. (2016). Bakteri Endofit pada La-mun di Pantai Litianak dan Oeseli, Rote Ndao, Nusa Tenggara Timur Penghasil Antibakteri terhadap Bakteri Methicil-lin Resistant Staphylococcus aureus (MRSA). Thesis. Surakarta: Bioscience, Universitas Sebelas Maret.

Santerre, J. P., Woodhouse, K., Laroche, G. & Labow, R.S. (2005). Understanding the Biodegradation of Polyurethanes: from Classical Implants to Tissue En-gineering materials. Biomaterials, 26, 7457–7470.

Shah, Z., Lee K., Deniz F.A., Fariha H.,Mu-tiullah K.& Aamer A.S.(2013). Degra-dation of Polyester Polyurethane by a Newly Isolated Soil Bacterium, Bacil-lus subtilis strain MZA-75. Biodegra-dation, 24, 865–877.

Whitman, W. B & Schleifer, K. H. (2009). Bergey’s Manual of Systematic Bacte-riology Second Edition Volume Three. The Firmicutes. New York: Springer.




DOI: https://doi.org/10.15575/biodjati.v4i2.4525

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