Application of Rhizoctonia mycorrhiza and without Rhizoctonia mycorrhiza in improving vegetative growth of Dendrobium nindii seedlings


R Soelistijono(1*), Daryanti Daryanti(2), Siti Mardhikasari(3), Tyara Yunika Sari(4), Dian Rakhmawati(5)

(1) Faculty of Agriculture Universitas Tunas Pembangunan, Indonesia
(2) Faculty of Agriculture Universitas Tunas Pembangunan, Indonesia
(3) Faculty of Agriculture Universitas Tunas Pembangunan, Indonesia
(4) Faculty of Agriculture Universitas Tunas Pembangunan, Indonesia
(5) Magelang District’s Agriculture and Food Service, Indonesia
(*) Corresponding Author

Abstract


Dendrobium is a genus of orchid that has the most attraction among the public rather than other types, especially for orchid species such as Dendrobium nindii type. The obstacle in cultivating orchids species is the slow vegetative growth when compared to orchids resulting from crosses (hybrid orchids). This research aimed to determine the vegetative growth of D. nindii seedlings using Rhizoctonia sp. The research was conducted at the Greenhouse, Faculty of Agriculture, Tunas Pembangunan University from January to July 2023. The research used quantitative descriptive method consisting of one treatment with six replications and each replications contained ten plants. The factor was application of Rhizoctonia mycorrhiza and without application of Rhizoctonia mycorrhiza. The results showed that (1) the morphological characteristics of Rhizoctonia mycorrhiza were white colonies, with right-angled branches and two nuclei, (2) the application of mycorrhizal Rhizoctonia had a very significant effect on the vegetative growth of D. nindii as shown in the parameters of plant height, leaf length, number of leaves, root length, number of roots and fresh weight of seedlings, and (3) there was an association of Rhizoctonia mycorrhiza with the formation of the peloton structure.

ABSTRAK

Dendrobium merupakan salah satu genus anggrek yang memiliki daya tarik paling banyak di masyarakat diantara jenis anggrek lainnya terutama untuk anggrek spesies seperti Dendrobium nindii. Kendala dalam budidaya anggrek spesies adalah pertumbuhan vegetatif yang lambat bila dibandingkan dengan anggrek hasil persilangan (anggrek hibrida). Penelitian ini bertujuan untuk mengetahui pertumbuhan vegetatif bibit D. nindii dengan menggunakan Rhizoctonia sp. Penelitian dilakukan di Rumahkasa Fakultas Pertanian Unversitas Tunas Pembangunan dari Januari sampai Juli 2023. Penelitian menggunakan metode deskriptif kuantitatif dengan enam ulangan dan setiap ulangan terdiri enam tanaman. Faktor perlakuan adalah aplikasi Rhizoctonia mikoriza dan tanpa aplikasi Rhizoctonia mikoriza. Hasil penelitian menunjukkan (1) ciri morfologi Rhizoctonia mikoriza berupa koloni berwarna putih, dengan percabangan siku-siku dan berinti dua, (2) pemberian Rhizoctonia mikoriza berpengaruh sangat nyata pada pertumbuhan vegetatif D. nindii  yang ditunjukan pada parameter tinggi tanaman, panjang daun, jumlah daun, panjang akar, jumlah akar dan berat segar bibit, dan (3) terjadi asosiasi Rhizoctonia mikoriza dengan terbentuknya struktur peloton.


Keywords


Dendrobium nindii, Rhizoctonia mycorrhiza, Peloton structure, Vegetative growth

Full Text:

PDF

References


Abdelghany, S. (2016). Introduction to Botany. https://www.researchgate.net/publication/299564526_Introduction_to_Botany

Agustini, Sufaati, V. S., Suharno. (2009). Mycorrhizal association of errestrial orchids of Cycloops Nature eserve, Jayapura. Biodiversitas, 10: 175-180. https://doi.org/10.13057/biodiv/d100403

Balestrini, R., Nerva, L., Sillo, F., Girlanda, M., & Perotto, S. (2014). Plant and fungal gene expression in mycorrhizal protocorms of the orchid Serapias vomeracea colonized by Tulasnella calospora. Plant Signaling & Behavior, 9 (11), e977707. https://doi.org/10.4161/15592324.2014.977707

Bierman, B., & Linderman, R. G. (1983). Use Vesikular-Arbuscular Myccorrhizal Roots. Radical Vesicles Plant Pathology. 63 (1) : 9-105. https://doi.org/10.1111/j.1469-8137.1983.tb03472.x

Calevo, J., & Duffy, K. J. (2023). Interactions among mycorrhizal fungi enhance the early development of a Mediterranean orchid. Mycorrhiza 33:229–240. https://doi.org/10.1007/s00572-023-01118-4

Dwiyanto, W., Soelistijono, R., & Utami, D. S. (2017). Rhizoctonia mikoriza anggrek vanda limbata characterization isolate the morphology and anatomy of orchid mycorrhizal Rhizoctonia Vanda limbata. Agreneca, 17(I), 1–11.

https://doi.org/10.36728/afp.v17i1.563

George, E. F., Hall, M. A., & De Klerk, G. J. (2008). Plant propagation by tissue culture 3rd Edition. Micropropagation: Uses and Methods, 1, 29–64. https://doi.org/10.1007/978-1-4020-5005-3_2

Gondal, A. S., Abdul, R., & Farah, N. (2019). Anastomosis groups of Rhizoctonia Solani associated with tomato foot rot in Pothohar Region of Pakistan. scientificreports. 9:3910 https://www.nature.com/articles/s41598-019-40043-5.pdf

Hatni, F. (2017). Karakterisasi planlet anggrek bulan (Phalaenopsis amabilis (L.) Bl.) hasil inokulasi Rhizoctonia sp. dan induksi asam salisilat secara in vitro. UNIVERSITAS LAMPUNG. https://digilib.unila.ac.id/26719/3/SKRIPSI%20TANPA%20BAB%20PEMBAHASAN.pdf

Herliana, O., Rokhminarsi, E., Mardini, S., & Jannah, M. (2018). Pengaruh jenis media tanam dan aplikasi pupuk hayati mikoriza terhadap pertumbuhan, pembungaan dan infeksi mikoriza pada tanaman anggrek Dendrobium sp. Kultivasi, 17(1), 550–557. https://doi.org/10.24198/kultivasi.v17i1.15774

Hossain, M. M., Rahi, P., Gulati, A., & Sharma, M. (2013). Improved ex vitro survival of asymbiotically raised seedlings of cymbidium using mycorrhizal fungi isolated from distant orchid taxa. Scientia Horticulturae, 159, 109–116. https://www.sciencedirect.com/science/article/abs/pii/S0304423813002203

Hossain, M. M. (2022). Orchid mycorrhiza: Isolation, culture, characterization and application. South African Journal of Botany. Vol. 151 : 365-384. https://doi.org/10.1016/j.sajb.2022.10.003

Kasiamdari, R. S., (2000). Binucleate Rhizoctonia isolate from mycorrhizal pot cultures: its morphological characteristics and pathogenicity. Berkala Ilmiah Biologi, 2

Latief, R.A., Hasibuan, S., & Mardiana, S. (2020). Stimulation of Growth and Development of Planlet Orchid (Dendrobium Sp) in The Acclimatization Stage by suplementing of Vitamin B1 and Atonik. Jurnal Ilmiah Pertanian (JIPERTA) 2 (2) : 127-134

Muthukumar, T., Uma, E., Pandey, R. (2013). Root morphology and mycotrophy of Disperis neilgherrensis (Orchidaceae) from Western Ghats, southern India. Anales de Biología 35: 89-94. https://pdfs.semanticscholar.org/92b8/b7c320fffa7e663147ff7c5e798b0c18192d.pdf

Ogura, Y., Yukawa, T., & Kinoshita, A. (2021). Evolutionary histories and mycorrhizal associations of mycoheterotrophic plants dependent on saprotrophic fungi. Journal of Plant Research 134 :19–41 https://doi.org/10.1007/s10265-020-01244-6

Oyetunde, O. O., & Bradley, C. A. (2017). Rhizoctonia solani: taxonomy, population biology and management of rhizoctonia seedling disease of soybean. Plant Pathology (67) p:3-17. https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.12733

Puccio P. (2018). Monaco Nature Encyclopedia. https://www.monaconatureencyclopedia.com/dendrobium-nindii-2/?lang=en

Sari, D. N. (2018). Identifikasi Orchid Mycorrhiza Pada Akar Anggrek Cattleya Sp. Dan Dendrobium Sp. (pp. 1–64). UIN Raden Intan Lampung. http://repository.radenintan.ac.id/3062/

Senthilkumar, S., Saravanakumar, P., Krishnamurthy, K. V., & Britto, J. (2001). Morphological and structural features of mycorrhizal roots of Spathoglottis plicata and Dendrobium species. Phyta, 5 (1), 24–31.

Siddiqui, Z. A., & Pichtel, J. (2008). Mycorrhiza: an overview. In Mycorrhiza: Sustainable Agriculture and Forestry (pp. 1–35). Springer. https://doi.org/10.1007/978-1-4020-8770-7_1

Smith, S. E., & Read, D. F. R. S. (2008). 12-The Mycorrhizas of Green Orchids. Academic Press. P: 419-457. https://www.sciencedirect.com/science/article/abs/pii/B9780123705266500143

Sneh, B., Yamoah, E., & Stewart, A. (2004). Hypovirulent Rhizoctonia spp. isolates from New Zealand soils protect radish seedlings against dampingoff caused by R. solani. New Zealand Plant Protection, 57, 54–58. https://doi.org/10.30843/nzpp.2004.57.6889

Soelistijono, Utami, D. S., & Priyatmojo, A. (2017). Identification of Rhizoctonia Mychorrizal and Fusarium on Ascocentrum Miniatum Orchid. BIOTA, 2: 7-13. https://ojs.uajy.ac.id/index.php/biota/article/view/1504

Soelistijono, R., Utami, D. S., Daryanti, Faizin, M., & Dian, R. (2020). Short communication: Characterization of Rhizoctonia-like mycorrhiza associated with five Dendrobium species in Java, Indonesia. Biodiversitas, 21(3), 1007–1011. https://doi.org/10.13057/biodiv/d210321

Suryantini, R., Wulandari R. S., & Kasiamdari R. S. (2015). Orchid Mycorrhizal Fungi: Identification of Rhizoctonia from West Kalimantan. MICROBIOLOGY INDONESIA. 9(4) p: 157-162. https://www.researchgate.net/publication/310329780_Orchid_Mycorrhizal_Fungi_Identification_of_Rhizoctonia_from_West_Kalimantan

Tuhuteru, S., Hehanusa, M. L., Raharjo, S. H. T. (2018). Pertumbuhan dan perkembangan anggrek Dendrobium anosmum pada beberapa konsentrasi air kelapa. Agrologia, 1(1) : 1-12. https://doi.org/10.30598/a.v1i1.293

Zimmer, K., Hynson, N. A., Gebauer, G., Allen, E. B., Allen, M. F., & Read, D. J. (2007). Wide geographical and ecological distribution of nitrogen and carbon gains from fungi in pyroloids and monotropoids (Ericaceae) and in orchids. New Phytologist, 175(1), 166–175. https://doi.org/10.1111/j.1469-8137.2007.02065.x




DOI: https://doi.org/10.15575/30844

Refbacks

  • There are currently no refbacks.


Creative Commons Licence

Jurnal Agro (J. Agro: ISSN 2407-7933) by http://journal.uinsgd.ac.id/index.php/ja/index is licensed under a Creative Commons Attribution 4.0 International License.