Antagonism potency of dark Septate endophytes against Pyricularia oryzae for improving health of rice plants


Dini Yuliani(1*), Bonny P. W. Soekarno(2), Abdul Munif(3), Surono Surono(4)

(1) Scopus ID: 57189629493, Balai Besar Penelitian Tanaman Padi, Indonesia
(2) ,  
(3) ,  
(4) ,  
(*) Corresponding Author

Abstract


Blast disease caused by Pyricularia oryzae (Po) is the main disease affecting rice production. Dark septate endophytes (DSEs) is known to improve plant performance and suppress disease. This study evaluated DSEs antagonism potency against P. oryzae in improving the rice plant's health. The research stages consisted of: (1). DSE and Po growth rate; (2). Antagonism of DSEs against Po; (3). Chitinase; (4). DSEs Viability; (5). DSEs application to rice seeds in nurseries. The results showed the growth of APDS 3.2 colonies had fulfilled Petri (d = 9 cm) at three days after incubation (DAI), while 4.1 BTG and TKC 2.2.a at 7 DAI.  Po had slow colony growth required 20 DAI. Inhibition of APDS 3.2 against Po was 43.75%, higher than of 4.1 BTG (38.60%) and of TKC 2.2.a (39.76%). The rice plants inoculated with APDS 3.2 had a relatively higher at seedling height, root length, wet weight, and dry weight than those inoculated with TKC 2.2.a and 4.1 BTG. The highest DSEs colonization was found in APDS 3.2 at 50.56%, followed by TKC 2.2.a (46.67%) and 4.1 BTG (40%). DSEs fungus has the potential to suppress rice blast pathogens by improving the health of rice plants, especially APDS 3.2.

Key words: Colonization, Growth Rate, Viability 

 

Penyakit blas yang disebabkan oleh Pyricularia oryzae (Po) merupakan penyakit utama yang memengaruhi produksi padi. Dark septate endophyte (DSE) diketahui mampu meningkatkan performa tanaman dan menekan penyakit. Penelitian ini bertujuan untuk mengevaluasi potensi antagonisme DSE terhadap P. oryzae dalam meningkatkan kesehatan tanaman padi. Tahapan Penelitian terdiri atas: (1). Kecepatan tumbuh DSE dan Po; (2). Antagonisme DSE terhadap Po; (3). Kitinase; (4). Viabilitas DSE; (5). Aplikasi DSE pada benih padi di persemaian. Hasil penelitian menunjukkan pertumbuhan koloni APDS 3.2 telah memenuhi petri (d= 9 cm) pada 3 hari setelah inkubasi (HSI), sedangkan 4.1 BTG dan TKC 2.2.a pada 7 HSI. Pertumbuhan koloni Po lambat membutuhkan 20 HSI. Penghambatan APDS 3.2 terhadap Po sebesar 43,75% lebih tinggi dibandingkan 4.1 BTG (38,60%) maupun TKC 2.2.a (39,76%). Tanaman padi yang diinokulasi APDS 3.2 memiliki tinggi, panjang akar, bobot basah, dan bobot kering relatif lebih tinggi dibandingkan yang diinokulasi TKC 2.2.a dan 4.1 BTG. Kolonisasi DSE tertinggi dijumpai pada APDS 3.2 sebesar 50,56%, diikuti TKC 2.2.a (46,67%) dan 4.1 BTG (40%). Cendawan DSE memiliki potensi untuk menekan patogen blas padi dengan cara meningkatkan kesehatan tanaman padi, khususnya APDS 3.2.


Keywords


Colonization, Growth Rate, Viability

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References


Agrios, G. N. (2005). Plant pathology. In Elsevier Academic Press (Fifth). Elsevier Academic Press. https://doi.org/10.1016/C2009-0-02037-6

Ahn, S.-N., Kim, Y.-K., Hong, H.-C., Han, S.-S., Soo-Jin Kwon1, H.-, Choi, C., Moon, H.-P., & McCouch, S. R. (2000). Molecular mapping of a new gene for resistance to rice blast (Pyricularia grisea Sacc.). Euphytica, 116, 17–22. https://doi.org/DOI: 10.1023/A:1004045812357

Amaria, W., Harni, R., & Samsudin. (2015). Evaluasi jamur antagonis dalam menghambat pertumbuhan Rigidoporus microporus penyebab penyakit jamur akar putih pada tanaman karet. Jurnal Tanaman Industri Dan Penyegar, 2(1), 51–60. https://doi.org/10.21082/jtidp.v2n1.2015.p51-60

Andrade-linares, D. R., Grosch, R., Franken, P., Rexer, K. H., Kost, G., Restrepo, S., de Garcia, M. C. C., & Maximova, E. (2011). Colonization of roots of cultivated Solanum lycopersicum by dark septate and other ascomycetous endophytes. Mycologia, 103(4), 710–721. https://doi.org/10.3852/10-329

Atugala, D. M., & Deshappriya, N. (2015). Effect of endophytic fungi on plant growth and blast disease incidence of two traditional rice varieties. Journal of the National Science Foundation of Sri Lanka, 43(2), 173–187. https://doi.org/10.4038/jnsfsr.v43i2.7945

Bashir, O., Khan, K., Hakeem, K. R., Mir, N. A., Rather, G. H., & Mohiuddin, R. (2016). Soil microbes diversity and root exudates as important aspects of rhizosphere ecosystem. In K. R. Hakeem & M. S. Akhtar (Eds.), Plant, Soil and Microbes: Volume 2: Mechanisms and Molecular Interactions (Issue January, pp. 337–357). Springer International Publishing Switzerland. https://doi.org/10.1007/978-3-319-29573-2

Dalimunthe, C. I., Soekarno, B. P., Munif, A., & Surono, S. (2019). Seleksi dan uji potensi cendawan Dark Septate Endophyte sebagai agensia hayati penyakit jamur akar putih (Rigidoporus microporus) pada tanaman karet. Jurnal Penelitian Karet, 37(1), 11–20. https://doi.org/10.22302/ppk.jpk.v37i1.624

Dewi, I. M., Cholil, A., & Muhibuddin, A. (2013). Hubungan karakteristik jaringan daun dengan tingkat serangan penyakit blas daun (Pyricularia oryzae Cav.) pada beberapa genotipe padi (Oryza sativa L.). Jurnal HPT, 1(2), 10–17. https://www.researchgate.net/publication/265164278

Elfina, Y. S., Dewi, R., & Ibrahim, R. (2013). Uji pelet biofungisida yang mengandung beberapa isolat Trichoderma sp. lokal Riau terhadap penyakit yang disebabkan oleh Ganoderma boninense pat. secara in-vitro. Prosiding Seminar Nasional “Peranan Teknologi Dan Kelembagaan Pertanian Dalam Mewujudkan Pembangunan Pertanian Yang Tangguh Dan Berkelanjutan”., November, 111–124. http://eksakta.ppj.unp.ac.id

Fadhil, L., Kadim, A., & Aljebory, A. M. (2014). Production of Chitinase by Serratia marcescens from Soil and Its Antifungal Activity. Journal of Natural Sciences Research Www, 4(8), 80–86. https://www.researchgate.net/publication/284550192

Fakhruddin, D. K., & Nurcahyanti, S. D. (2020). Viabilitas Bacillus sp. sebagai agen antagonis patogen tanaman dalam formulasi berbahan dasar tepung. Jurnal Pengendalian Hayati, 3(1), 29–37. https://doi.org/10.19184/jph.v3i1.17151

Fukuta, Y., Koga, I., Ung, T., Sathya, K., Kawasaki-Tanaka, A., Koide, Y., Kobayashi, N., Obara, M., Yadana, H., & Hayashi, N. (2014). Pathogenicity of rice blast (Pyricularia oryzae Cavara) isolates from Cambodia. JARQ, 48(2), 155–166.

Gayatonde, V., Mahadevu, P., & Vennela, P. R. (2016). Study of suitable culture media and other abiotic factors for the growth and sporulation of Magnaporthe oryzae. Ecology, Environment and Conservation, 22(2), 297–301.

Gougouli, M., & Koutsoumanis, K. (2013). Primary models for fungal growth. In P. D (Ed.), Predictive Mycology (1st ed., Issue January 2013, pp. 63–130). Nova Science. https://www.researchgate.net/publication/280729007

Handayani, D. (2017). Karakteristik cendawan dark septate endophyte (DSE) pada akar tanaman jagung dan padi. Eksakta, 18(1), 61–68. http://eksakta.ppj.unp.ac.id

He, C., Wang, W., & Hou, J. (2019). Characterization of dark septate endophytic fungi and improve the performance of liquorice under organic residue treatment. Frontiers in Microbiology, 10(June), 1–14. https://doi.org/10.3389/fmicb.2019.01364

Herre, E. A., Mejía, L. C., Kyllo, D. A., Rojas, E. I., Maynard, Z., Butler, A., & Van Bael, S. A. (2007). Ecological implications of anti-pathogen effects of tropical endophytes and mycorrhizae. Ecology, 88(3), 550–558. https://doi.org/10.1890/05-1606

Hosseini-moghaddam, M. S., & Soltani, J. (2013). An investigation on the effects of photoperiod, aging and culture media on vegetative growth and sporulation of rice blast pathogen Pyricularia oryzae. Progress in Biological Sciences, 3(2), 135–143.

Khare, E., Mishra, J., & Arora, N. K. (2018). Multifaceted interactions between endophytes and plant: developments and prospects. Frontiers in Microbiology, 9(Nov), 1–12. https://doi.org/10.3389/fmicb.2018.02732

Khastini, R. O. (2007). Isolasi, penapisan, respon tumbuh dan proses kolonisasi cendawan mutualistik akar (Tesis). Institut Pertanian Bogor.

Knapp, D. G., Pintye, A., & Kovács, G. M. (2012). The dark side is not fastidious - dark septate endophytic fungi of native and invasive plants of semiarid sandy areas. PLoS ONE, 7(2), 1–8. https://doi.org/10.1371/journal.pone.0032570

Maharshi, A. R., & Thaker, V. S. (2012). Growth and development of plant pathogenic fungi in define media. European Journal of Experimental Biology, 2(1), 44–54. www.pelagiaresearchlibrary.com Pelagia

Milati, L. N., Nasution, A., & Sudir. (2016). Metode identifikasi Ras Pyricularia grisea Sacc. penyebab penyakit blas pada tanaman padi. In S. et al. Abdulrachman (Ed.), Prosiding Temu Teknologi Padi 2015 (pp. 429–436). Balai Besar Penelitian Tanaman Padi.

Muharni. (2009). Isolasi dan identifikasi bakteri penghasil kitinase dari sumber air panas danau Ranau Sumatera Selatan. Jurnal Penelitian Sains, 9(D), 12–15.

Nurbaya, Kuswinanti, T., Baharuddin, Rosmana, A., & Millang, S. (2010). Uji kecepatan pertumbuhan Fusarium spp. pada media organik dan media sintesis. Jurnal Bionature, 15(1), 45–53.

Pandya, U., & Saraf, M. (2010). Application of fungi as a biocontrol agent and their biofertilizer potential in agriculture. Research Journal of Biotechnology, 5(3), 5–9. https://www.researchgate.net/publication/292574989%0AApplication

Prayogo, Y., & Santoso, T. (2013). Viabilitas dan infektivitas formulasi cendawan entomopatogen Lecanicillium lecanii sebagai biopestisida pengendalian telur kepik coklat Riptortus linearis. Penelitian Pertanian Tanaman Pangan, 32(1), 57–66.

Santos, S. G. dos, Silva, P. R. A. da, Garcia, A. C., Zilli, J. É., & Berbara, R. L. L. (2017). Dark septate endophyte decreases stress on rice plants. Brazilian Journal of Microbiology, 48(2), 333–341. https://doi.org/10.1016/j.bjm.2016.09.018

Sesma, A., & Osbourn, A. E. (2004). The rice leaf blast pathogen undergoes developmental processes typical of root-infecting fungi. Nature, 431(7008), 582–586. https://doi.org/10.1038/nature02880

Siagian, A. (2016). Gejala anatomi dan kerusakan penyakit blas (Pyricularia oryzae) pada tanaman padi. Institut Pertanian Bogor.

Sucipto, I., Munif, A., Suryadi, Y., & Tondok, E. T. (2015). Eksplorasi cendawan endofit asal padi sawah sebagai agens pengendali penyakit blas pada padi sawah. Jurnal Fitopatologi Indonesia, 11(6), 211–218. https://doi.org/10.14692/jfi.11.6.211

Sudha, V., Govindaraj, R., Baskar, K., Al-Dhabi, N. A., & Duraipandiyan, V. (2016). Biological properties of endophytic Fungi. Brazilian Archives of Biology and Technology, 59, 1–7. https://doi.org/10.1590/1678-4324-2016150436

Sudir, Nasution, A., Santoso, & Nuryanto, B. (2014). Penyakit blas Pyricularia grisea pada tanaman padi dan strategi pengendaliannya. Iptek Tanaman Pangan, 9(2), 85–96.

Suganda, T., Yulia, E., Widiantini, F., & Hersanti, H. (2016). Intensitas penyakit blas (Pyricularia oryzae Cav.) pada padi varietas Ciherang di lokasi endemik dan pengaruhnya terhadap kehilangan hasil. Agrikultura, 27(3), 154–159. https://doi.org/10.24198/agrikultura.v27i3.10878

Surono, & Narisawa, K. (2018). The inhibitory role of dark septate endophytic fungus Phialocephala fortinii against Fusarium disease on the Asparagus officinalis growth in organic source conditions. Biological Control, 121, 159–167. https://doi.org/10.1016/j.biocontrol.2018.02.017

Syarif, A. S., Nurjayadi, M. Y., & Munif, A. (2014). Isolasi cendawan endofit dari tanaman padi dan potensinya sebagai pemacu pertumbuhan tanaman. In N. Mubin, M. G. Pradana, Suryadi, M. Y. Nurjayadi, & D. Sukaryana (Eds.), Prosiding Seminar Nasional Perlindungan Tanaman II (pp. 132–138). Pusat Kajian Pengendalian Hama Terpadu.

Vergara, C., Araujo, K. E. C., Urquiaga, S., Santa-catarina, C., Schultz, N., Araujo, E. da S., Balieiro, F. de C., Xavier, G. R., & Zilli, J. E. (2018). Dark septate endophytic fungi increase green manure- 15N recovery efficiency , N contents , and micronutrients in rice grains. Frontier in Plant Science, 9, 1–11. https://doi.org/10.3389/fpls.2018.00613

Yulianti, T. (2013). Pemanfaatan endofit sebagai agensia pengendali hayati hama dan penyakit tanaman. Buletin Tanaman Tembakau, Serat & Minyak Industri, 5(1), 40–49. https://doi.org/10.21082/bultas.v5n1.2013.40-49

Zarandi, M. E., Bonjar, G. H. S., Dehkaei, F. P., Moosavi, S. A. A., Farokhi, P. R., & Aghighi, S. (2009). Biological control of rice blast (Magnaporthe oryzae) by use of Streptomyces sindeneusis isolate 263 in greenhouse. American Journal of Applied Sciences, 6(1), 194–199.

Zhang, H., Wu, Z., Wang, C., Li, Y., & Xu, J. (2014). Germination and infectivity of microconidia in the rice blast fungus Magnaporthe oryzae. Nature Communications, 5, 1–9. https://doi.org/10.1038/ncomms5518

Zhang, Y., Li, T., & Zhao, Z. (2011). The colonization of plants by dark septate endophytes (DSE) in the valley-type savanna of Yunnan, southwest China. African Journal of Microbiology Research, 5(31), 5540–5547. https://doi.org/10.5897/ajmr10.389




DOI: https://doi.org/10.15575/9589

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