Pengaruh Fungi Indigenous Toleran Zn terhadap Pertumbuhan Bibit Jagung di Media Tailing Steril

Ratna Santi, Benny Joy, Regina Hindersah, Dedi Nusyamsi

Abstract


Keberadaan logam Zn dalam jumlah tertentu di tailing pasca tambang akan berdampak pada rendahnya populasi mikroba tanah dan menghambat pertumbuhan tanaman. Aplikasi pemanfaatan fungi indigenus dari lahan tercemar merupakan salah satu usaha dalam memperbaiki sifat tanah untuk pertumbuhan tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh inokulasi fungi dan toksisitas Zn terhadap pertumbuhan jagung pada fase VE-V9 di media tailing steril.  Fungi diisolasi dari tailing lahan pasca penambangan timah di Sungailiat  Bangka. Tiga isolat dari 15 isolat dipilih untuk pengujian pengaruh inokulasi fungi terhadap pertumbuhan jagung. Percobaan menggunakan Rancangan Acak Kelompok(RAK) dua faktor dengan perlakuan jenis fungi dan konsentrasi Zn. Isolat yang digunakan toleran terhadap Zn pada konsentrasi 0-25 ppm dan mampu menghasilkan fitohormon. Hasil percobaan di rumah kaca menunjukkan bahwa inokulasi fungi nyata memperbaiki pertumbuhan jagung, dibandingkan tanpa inokulan. Serapan tertinggi ditunjukkan oleh isolat R 7J1, namun pertumbuhan jagung terbaik didapatkan dari inokulasi isolat B 2J1.

 

The existence of Zn metal in a certain amount in the post tin mine tailings will result in low soil microbial populations and inhibit plant growth. Application of indigenous fungi utilization on contaminated land is one effort to improve soil properties for plant growth.This study aimed to determine the effect of inoculation of fungi and toxicity of zinc on the growth of corn in the phase of VE-V9 in sterile tailings medium. Fungi were isolated from post tin mining tailings tin lands in Bangka Sungailiat. Three isolates from 15 isolates were selected to test the effect of fungal inoculation on the growth of corn. Experiment used a Randomized Complete Block Design (RCBD) two factors with fungi and Zn concentration treatments.Tolerant isolates used were at a concentration of 0-25 ppm Zn and capable to produce phytohormones. Result of experiment in greenhouse showed that fungal inoculation substantially improved the growth of maize, compared with no inoculant.The highest uptake was shown by isolates of R 7J1, but the best corn growth inoculation isolates obtained from B 2J1.


Keywords


Fungi; Jagung; Tailing; Zn

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References


Baca, B.E. and C. Elmerich. 2003. Microbial Production of Plant Hormones. In C.Elmerich and W.E. Newton (eds.), Associative and Endophytic Nitrogen-fixing Bacteria and Cyanobacterial Associations. Kluwer Akademic Publishers. Netherlands.

Couder, E. 2011. Zn Biogeochemical cycle in highly Zn-contaminated soil-plant system. Universite Catholique –Louvain.

Cui, Y. and N. Zhao. 2011. Oxidative stress and change in plant metabolisme of maize (Zea mays. L) growing in contaminated soil with elemental sulfur and toxic effect of Zinc. Plant Soil Environ. Vol. 57 (1):34-39

Duruibe, J.O., Ogwuegbu, M.O.C and Egwurugwu, J.N. 2007. Heavy metal pollution and human biotoxic effects. Leghth Reseach Paper. International Physical Science, 2(5): p.112-118

Iram., S., Ahmad, S., Javed., B., Yaqoob, S., Akhtar, K., Kazmi,M.Z and Badar-uz-zaman. 2009. Fungal Toleransi Heavy Metals. J Botany , 41(5): p.2583-2594

Jadia, C.D and M. Fulekar. 2008. Pytoremediation. The aplication of vermecompost to remove Zn, Cd, Cu, Ni and Pb by sunflower plant. J. Enviromental Engineering and Management. Vol. 7 (5):547–558.

Kabata-Pendias, A and Mukherjee, A.B. 2007. Trace Elements from Soil to Human. New york : Springer-Verlag Berlin Heiderlberg.

Kuffner, M., Puschenreiter, M., Wieshammer, G., Gorfer, M and Sessitch, A. 2008. Rhizosphere bacteria affect growth and metal upatake of heavy metal accumulating willows. Plant Soil, 304 : p.35-44

Lahuddin. 2007. Aspek Unsur Mikro dalam Kesuburan Tanah. USU e-Repository. Universitas Sumatera Utara Medan.

Lasad, M.M and EPA, 2009. The Use Plants for Removal of Toxic Metals from Contaminan Soil. America Asosiation for the Advancement of science and Enviromental Ecience and Engineering Fellow. Enviromental Protection Agency (EPA) Published.

Pattern, C.L and Glick, B.R. 2002. Role of Pseudomonas putida indole acetic acid in development of the plant root system. App Enviro Microbial, 63: p.3795-3801

Reichman, S.M. 2002. The Responses of Plants to Metal Toxicity. Focusing on Copper, Manganese and Zinc. Review. Published by Australian Minerals and Energy Enviroment Foundation Published.

Soudek, P., S. Petrova, S, D. Benesova and T. Vanek. 2010. Phytoextraction of toxic metal by sun flower and corn plants. J. Food Agriculture and Enviromental. Vol 8, Issues 3-4:383- 390.

Subekti, N.A., Safruddin, Efendy, R dan Sunarti, S. 2008. Morfologi Tanaman dan Fase pertumbuhan Jagung. Balai Penelitian Tanaman Serealia. Maros.

Sagardoy, R., Morales, F., Lofez-Milan, A.F., Abadia, A and Abadia, J. 2008. Effects of zinc toxicity on sugar beet (beta vulgaris L.) plant grown in hydroponics. Reseach Paper. J Plant Biology , 11: p.339-350

Sulaeman, Eviati, H. Sastramihardja, S.E. Aprilani dan M. Farida. 2009. Penetapan Unsur Logam Berat Total Cara Pengabuan Basah dengan HNO3 dan HClO4. p. 110-113.. In Balai Besar Litbang Sumberdaya Lahan Pertanian (ed.). Analisis Kimia Tanah, Tanman, Air dan Pupuk. Balai penelitian dan Pengembangan Pertanian, Jakarta Selatan

Yan-de, J., Zhen-li, H dan Ziao-e, Y. 2007. Raloe of soil rhizobacteria in phytoremediation of heavy metal contaminated soils. J Zhejiang University Science, 8(3): p.19-25.




DOI: https://doi.org/10.15575/157

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