Sunscreen Activity Determination on Hippobroma Longiflora Leaves Extracts Affected to Differences in Simplicia Treatments and Extraction Techniques

Authors

  • Awaly Ilham Dewantoro Department of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Indonesia
  • Selly Harnesa Putri Department of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Indonesia
  • Efri Mardawati Department of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Indonesia
  • Desy Nurliasari Department of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Indonesia

DOI:

https://doi.org/10.15575/ak.v9i2.20502

Keywords:

Infusion, Kitolod, Maceration, Polyphenols Content, SPF.

Abstract

Various weeds in paddy rice fields could be utilized as natural polyphenols sources, one of them was kitolod (Hippobroma longiflora (L.) G.Don). Kitolod was widely utilized as traditional medicine by several community groups because of its polyphenols content. Recent trends showed kitolod could be valorized as additive matters in sunscreen products. Polyphenols content, especially flavonoids were able to absorb dangerous spectrum in sunray radiation and it is related to sunscreen activity. This study aimed to evaluate the differences in simplicia treatments and extraction techniques towards total polyphenols content and sunscreen activity on kitolod leaf extracts. Solvent-cold extraction techniques were deployed in this study that consist of water infusion and ethanolic maceration for 24 hours at ambient temperature. Extracts were analyzed by spectrophotometric measurement to determine total phenolics content (TPC), total flavonoids content (TFC), and sunscreen activity by deploying Folin-Ciocalteu assay, AlCl3 colorimetric assay, and Mansur approached assay, respectively. Dry kitolod leaves were extracted by ethanolic maceration techniques provided high yields (15.5±0.3%), high TPC (17.9±0.3 mg GAE/g of solids), high TFC (25.9±0.4 mg QE/g of solids), and moderate sunscreen activity (SPF 11.0±0.1). The results indicated kitolod could be valorized as natural polyphenols sources and additive matters in any cosmetical sunscreen products.

Author Biographies

Selly Harnesa Putri, Department of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, Universitas Padjadjaran

Department of Agroindustrial Technology, Faculty of Agro-Industrial Technology

Efri Mardawati, Department of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, Universitas Padjadjaran

Department of Agroindustrial Technology, Faculty of Agro-Industrial Technology

Desy Nurliasari, Department of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, Universitas Padjadjaran

Department of Agroindustrial Technology, Faculty of Agro-Industrial Technology

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Published

2023-01-01

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