Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum

Indah Juwita Sari, Indria Wahyuni, Rida Oktorida Khastini, Ewi Awaliyati, Andriana Susilowati, Enggar Utari, I Nyoman Pugeg Aryantha

Abstract


Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.


Keywords


Capsicum annuum, Characterization, Chilli Plants, Dual Culture, PGPR.

Full Text:

PDF

References


Badan Pusat Statistik [BPS]. (2016). Produktivitas Cabai Besar. Retrieved from http://www.pertanian.go.id/Data5tahun/HortiASEM2016(pdf)/Produksi%20Cabai%20Besar.pdf.

Bai, A.T., Ruth, Ch., Gopal, K., Arunodhayam, K., Priya, B. T. & Ramakrishna, M. (2018). Survey and Identification of Fusarium Wilt Disease in Chilli (Capsicum annuum L.). Int.J.Curr.Microbiol, 7(6), 1073-1078.

Batool, S. & Altaf, M. A. (2017). Plant Growth Promoting Rhizobacteria (PGPR) Reduces Application Rates of Fertilizers in Chili (Capsicum frutescens L.) Cultivation. J Hortic, 4(215), 2376-0354,

Bunbury-Blanchette, A. L. & Walker, A. K. (2019). Trichoderma Species Show Biocontrol Potential in Dual Culture and Greenhouse Bioassays Against Fusarium Basal Rot of Onion. Biological Control, 130, 127-135.

Cappuccino, J.G. & Sherman, N. (2004). Microbiology: A Laboratory Manual. Singapore: Pearson Education.

Fukami. J., Cerezini, P. & Hungria, M. (2018). Azospirillum: Benefits that Go Far Beyond Biological Nitrogen Fixation. AMB Express, 8(1), 73.

Holt, J. G., Kreig, N. R., Sneath, P. H. A., Staley, J. T. & Williams, S. T. (1994). Bergey’s Manual of Determinative Bacteriology 9th. United States of America: Williams & Wilkins.

Joshi, M., Srivastava, R., Sharma, A. K. & Prakash, P. (2012). Screening of Resistant Varieties and Antagonistic Fusarium Oxysporum for Biocontrol of Fusarium Wilt of Chilli. Journal of J Plant Pathology & Microbiology, 3(5), 1-6.

Kanchana, D., Jayanthi, M., Saranraj, P. & Sujitha, D.. (2013). Prevelence of Azotobacter sp. in Chilli (Capsicum annuum L.) Rhizosphere Soil of Cuddalore District, Tamil Nadu, India. International Journal of Microbiological Research, 4(3), 296-299.

Martínez O. A., Encina, C., Tomckowiack, C., Droppelmann, F., Jara, R., Maldonado, C., Muñoz, O., Fraile, P. G. & Rivas, R. (2018). Serratia Strains Isolated From The Rhizosphere of Raulí (Nothofagus Alpina) in Volcanic Soils Harbour PGPR Mechanisms and Promote Raulí Plantlet Growth. J. Soil Sci. Plant Nutr, 8(3), 804-819.

Mmbaga, M. T., Gurung, S. & Maheshwari. (2018). Screening of Plant Endophytes as Biological Control Agents Against Root Rot Pathogens of Pepper (Capsicum annum L.). J Plant Pathol Microbiol, 9(3), 1-8.

Munir. I., Bano, A. & Faisal, M. (2019). Impact of Phosphate Solubilizing Bacteria On Wheat (Triticum aestivum ) in The Presence of Pesticides. Braz. J. Biol, 79(1), 29-37.

Rajkumar, K., Naik, M. K. Amaresh, Y. S. & Chennappa, G. (2018). Bioefficacy of Bacillus subtilis Against Major Pathogen of Chilli Colletotrichum capsici Causing Fruit Rot of Chilli. Int.J.Curr.Microbiol. App.Sci, 7(7), 2681-2686.

Rupela, O. P., Gopalakrishnan, S., Krajewski, M. & Sriveni, M. (2003). A Novel Method For The Identification And Enumeration of Microorganisms With Potential for Suppressing Fungal Plant Pathogens. Biology and fertility of soils, 39(2), 131-134.

Sari, I. J. & Aryantha, I. N. P. (2021). Screening and Identification of Mushrooms Growth Promoting Bacteria on Straw Mushrooms (Volvariella volvacea). Journal of Tropical Biodiversity and Biotechnology, 6(1), 60618.

Saxena, A., Raghuvanshi, R. & Singh, H. B. (2014). Molecular, Phenotypic And Pathogenic Variability in Colletotrichum Isolates of Subtropical Region in North Eastern India, Causing Fruit Rot Of Chillies. J. Appl. Microbiol, 117, 1422–1434.

Saxena, A., Raghuwanshi, R., Gupta, V. K. & Singh, H. B. (2016). Chilli Anthracnose: The Epidemiology and Management. Frontiers in Microbiology, 7, 1527.

Schillaci, M., Gupta, S., Walker, R. & Roessner, U. (2019). The Role of Plant Growth Promoting Bacteria in Growth of Cereals under Abiotic Stresses. Root Biology - Growth, Physiology, and Functions. Retrieved from https://www.intechopen.com/chapters/67771.

Shobha, G. & Kumudini, B. S. (2012). Antagonistic effect of the Newly Isolated PGPR Bacillus spp. on Fusarium oxysporum. Int J Appl Sci Eng Res, 1(3), 463-474.

Sutariati, G. A. K. & Wahab, A. (2010). Isolasi dan Uji Kemampuan Rizobakteri Indigenous Sebagai Agensia Pengendali Hayati Penyakit pada Tanaman Cabai. J. Hort, 20(1), 86--95.

Suwan. N., To-anun, C., Soytong, K. & Nalumpang, S. (2012). Evaluation of Streptomyces-biofungicide to Control Chili Anthracnose in Pot Experiment. Journal of Agricultural Technology, 8(5), 1663-1676.

Than, P. P., Prihastuti, H., Phoulivong, S., Taylor, P. W. J. & Hyde, K. D. (2008). Chilli Anthracnose Disease Caused by Colletotrichum species. Journal of Zhejiang University Science B, 9(10), 764–778.

Thilagam, R. & Hemalatha, N. (2019). Plant Growth Promotion and Chilli Anthracnose Disease Suppression Ability of Rhizosphere Soil Actinobacteria. Journal of Applied Microbiology, 126 (6), 1835-1849.

Vejan. P., Abdullah, R., Khadiran, T., Ismail, S. & Boyce, A.N. (2016). Role of Plant Growth Promoting Rhizobacteria in Agricultural Sustainability—A Review. Molecules, 21(5), 573.

Widiyanti, D., Pujiyanto, S., Suprihadi, A. & Setyowati, M.. (2016). Penapisan dan Pemanfaatan Rhizobakteri Tanaman Jagung (Zea Mays) Sebagai Inokulan Pemacu Tumbuh Tanaman. Jurnal Biologi, 5(3), 80—89.

Živković, S., Stojanović, S., Ivanović, Ž., Gavrilović, V., Popović, T. & Balaž, J. (2010). Screening of Antagonistic Activity of Microorganisms Against Colletotrichum Acutatum And Colletotrichum Gloeosporioides. Arch. Biol. Sci., Belgrade, 62(3), 611-623.




DOI: https://doi.org/10.15575/biodjati.v6i2.13191

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Jurnal Biodjati



Indexing By :

      

      

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

View My Stats