Eksplorasi dan karakterisasi keragaman plasma nutfah tanaman padi (Oryza sativa L.) di pulau Belitung


Yuditia Arta Kencana(1), Eries Dyah Mustikarini(2*), Tri Lestari(3)

(1) ,  
(2) Bangka Belitung University, Indonesia
(3) ,  
(*) Corresponding Author

Abstract


Banyak sumber daya genetik penting yang masih belum teridentifikasi di Pulau Belitung salah satunya adalah tanaman padi. Kegiatan eksplorasi dan identifikasi menjadi langkah yang tepat untuk mendapatkan jenis tanaman padi baru pada kegiatan pemuliaan tanaman. Tujuan penelitian ini adalah melakukan eksplorasi, karakterisasi, dan menentukan hubungan kekerabatan dan variabilitas padi di Pulau Belitung. Penelitian dilaksanakan dari bulan Desember 2020 hingga Mei 2021. Penelitian menggunakan metode eksplorasi dengan teknik pengambilan sampel secara purposive sampling. Karakter yang diidentifikasi terdiri dari karakter kualitatif dan karakter kuantitatif. Analisis kekerabatan menggunakan program  NTSYS. Hasil penelitian didapatkan lima aksesi yaitu Rembiak, Siam, Cerai Merah, Ketan dan Merawang. Hasil uji beda nyata terkecil (BNT) menunjukkan aksesi padi lokal Belitung memiliki perbedaan yang nyata pada karakter umur panen (α 5%). Hasil analisis hubungan kekerabatan pada karakter kualitatif terdapat 2 grup dengan koefisien 0,64 atau 64%, kuantitatif terdapat 4 grup dengan koefisien 0,28 atau 28% dan gabungan dari karakter kualitatif dan kuantitatif terdapat 4 grup dengan koefisien 0,33 atau 33%. Aksesi padi yang diperoleh terdapat variabilitas genetik luas yaitu pada karakter jumlah biji total serta variabilitas fenotip yang luas yaitu pada tinggi tanaman, umur panen dan berat 1000 benih.

ABSTRACT

Many important genetic resources have not identified yet on the Belitung Island, one of which is rice plant. Exploration and identification activities are the right steps to get a new type of rice plant in plant breeding activities. The objectives of research were to explore, characterize, and determine the relationship and variability of rice on Belitung Island. The experiment was conducted from December 2020 to May 2021. Research used exploratory  methods with purposive sampling technique.The identified character consisted of qualitative and quantitative characters. Kinship analysis using the NTSYS program. The results of the study obtained five accessions namely Rembiak, Siam, Cerai Merah, Ketan and Merawang. Least Significance Different (LSD) results showed that local rice accession had a noticeable difference in the character of the harvest age (α 5%)." The results of the analysis of relationships in qualitative character there were 2 groups with coefficients of 0.64 or 64%, quantitative there were 4 grup with coefficients of 0.28 or 28% and a combination of qualitative and quantitative characters there were 4 groups with coefficients of 0.33 or 33%. Rice accession obtained contained extensive genetic variability in the character of the total number of seeds as well as wide phenotype variability on the plant height, harvest age and weight of 1000 seeds.


Keywords


Belitung, Eksplorasi, Keragaman, Plasma Nutfah, Padi

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References


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DOI: https://doi.org/10.15575/15085

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SELECT * FROM metadata_descriptions WHERE assoc_type = 1048579 AND assoc_id = 288492 Speed: 0.315 ms
SELECT * FROM citation_settings WHERE citation_id = 288493 Speed: 0.337 ms
SELECT * FROM metadata_descriptions WHERE assoc_type = 1048579 AND assoc_id = 288493 Speed: 0.295 ms
SELECT * FROM citation_settings WHERE citation_id = 288494 Speed: 0.324 ms
SELECT * FROM metadata_descriptions WHERE assoc_type = 1048579 AND assoc_id = 288494 Speed: 0.299 ms
SELECT * FROM citation_settings WHERE citation_id = 288495 Speed: 0.389 ms
SELECT * FROM metadata_descriptions WHERE assoc_type = 1048579 AND assoc_id = 288495 Speed: 0.451 ms
SELECT * FROM citation_settings WHERE citation_id = 288496 Speed: 0.446 ms
SELECT * FROM metadata_descriptions WHERE assoc_type = 1048579 AND assoc_id = 288496 Speed: 0.383 ms
SELECT * FROM citation_settings WHERE citation_id = 288497 Speed: 0.371 ms
SELECT * FROM metadata_descriptions WHERE assoc_type = 1048579 AND assoc_id = 288497 Speed: 0.358 ms
SELECT * FROM citation_settings WHERE citation_id = 288498 Speed: 0.354 ms
SELECT * FROM metadata_descriptions WHERE assoc_type = 1048579 AND assoc_id = 288498 Speed: 0.368 ms
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SELECT * FROM metadata_descriptions WHERE assoc_type = 1048579 AND assoc_id = 288499 Speed: 0.390 ms
SELECT * FROM citation_settings WHERE citation_id = 288500 Speed: 0.376 ms
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SELECT pa.*, a.*, COALESCE(stl.setting_value, stpl.setting_value) AS section_title, COALESCE(sal.setting_value, sapl.setting_value) AS section_abbrev FROM published_articles pa INNER JOIN articles a ON pa.article_id = a.article_id LEFT JOIN sections s ON s.section_id = a.section_id LEFT JOIN section_settings stpl ON (s.section_id = stpl.section_id AND stpl.setting_name = title AND stpl.locale = en_US) LEFT JOIN section_settings stl ON (s.section_id = stl.section_id AND stl.setting_name = title AND stl.locale = en_US) LEFT JOIN section_settings sapl ON (s.section_id = sapl.section_id AND sapl.setting_name = abbrev AND sapl.locale = en_US) LEFT JOIN section_settings sal ON (s.section_id = sal.section_id AND sal.setting_name = abbrev AND sal.locale = en_US) INNER JOIN article_settings ast ON a.article_id = ast.article_id WHERE ast.setting_name = pub-id::publisher-id AND ast.setting_value = 15085 Speed: 3.664 ms
SELECT pa.*, a.*, COALESCE(stl.setting_value, stpl.setting_value) AS section_title, COALESCE(sal.setting_value, sapl.setting_value) AS section_abbrev FROM published_articles pa, articles a LEFT JOIN sections s ON s.section_id = a.section_id LEFT JOIN section_settings stpl ON (s.section_id = stpl.section_id AND stpl.setting_name = title AND stpl.locale = en_US) LEFT JOIN section_settings stl ON (s.section_id = stl.section_id AND stl.setting_name = title AND stl.locale = en_US) LEFT JOIN section_settings sapl ON (s.section_id = sapl.section_id AND sapl.setting_name = abbrev AND sapl.locale = en_US) LEFT JOIN section_settings sal ON (s.section_id = sal.section_id AND sal.setting_name = abbrev AND sal.locale = en_US) WHERE pa.article_id = a.article_id AND a.article_id = 15085 Speed: 0.884 ms
Speed: 0.554 ms
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Speed: 0.440 ms
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17.61 MB

Used Memory

128M

Total Available

503.89 kB string: PKPApplication::construct
513

Total Files

2.77 MB

Total Size

113.87 kB

Largest

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