Bioassay of phosphorus solubilizing isolates for enhance P solubility and growth of rice (Oryza sativa L.)


Betty Natalie Fitriatin(1*), Dahlia Florencia Manurung(2), Mieke Rochimi Setiawati(3)

(1) Universitas Padjadjaran, Indonesia
(2) ,  
(3) ,  
(*) Corresponding Author

Abstract


Fosfat merupakan salah satu unsur yang berperan penting bagi pertumbuhan tanaman dan kesuburan tanah. Namun, ketersediaan unsur P terlarut yang dapat diserap oleh tanaman sangat kecil karena berikatan dengan kation yang berada di dalam tanah. Salah satu upaya dalam meningkatkan P tersedia dalam tanah adalah dengan pemanfaatan agen hayati Bakteri Pelarut Fosfat (BPF). Penelitian ini bertujuan untuk menguji isolat BPF yang dapat meningkatkan kelarutan P dan pertumbuhan padi pada uji hayati. Percobaan ini dilaksanakan di Rumah Kaca Departemen Ilmu Tanah dan Sumber Daya Lahan, Fakultas Pertanian, Universitas Padjadjaran. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan 6 perlakuan dan 5 ulangan. Masing-masing perlakuan jenis bakteri adalah kontrol, Bacillus substilis, B. megatherium, Pseudomonas mallei, Burkholderia sp., dan isolat campuran. Hasil percobaan menunjukkan isolat BPF yang diuji memiliki kemampuan yang bervariasi dalam meningkatkan enzim fosfatase, kelarutan P, dan pertumbuhan padi pada uji hayati. Lebih lanjut, perlakuan BPF campuran memberikan pengaruh lebih baik terhadap aktivitas fosfatase, P terlarut dan pertumbuhan padi dibandingkan isolat tunggal.

ABSTRACT

Phosphorus is an element that important for soil fertility and plant growth. However, the phosphate nutrient can be uptaken by plants only in a small amount because it binds to cations in the soil. The effort for enhancing the soil P availabilty is by the phosphorus solubilizing bacteria (PSB). This study aimed to test PSB  isolates  for increasing P solubility and rice growth using bioassay. The experiment conducted at the greenhouse in Jatinangor District, Sumedang Regency, West Java with  Randomized Block Design (RBD) for PSB isolates with five replications. Each type of bacteria treatment was control, Bacillus substilis, B. megatherium, Pseudomonas mallei, Burkholderia sp., and mixed isolates. The results showed that the P solubilizing  isolates had various abilities to enhance phosphatase, P solubility, and rice growth using bioassay. Furthermore, the mixed PSB isolates had a better effect on phosphatase activity, dissolved P and rice growth than single isolates.


Keywords


Bioassay, P Dissolved, Isolate, Phosphatase

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References


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DOI: https://doi.org/10.15575/17754

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