Eksplorasi aktinobakteria indigenus untuk Pengendalian penyakit busuk tongkol oleh Fusarium verticillioides pada tanaman jagung

Tifla Fitri Annisa, Yulmira Yanti, Nurbailis Nurbailis

Abstract


Fusarium verticillioides is a fungus that causes cob rot disease in corn plants. Control of Fusarium verticilliodes by using biological agents that are antagonistic, namely actinobacteria. The research aims to obtain actinobacteria isolates that can control cab rot disease and increase corn growth. The research consisted of 3 stages, 1.) Isolation of indigenous actinobacteria and F.verticillioides. Variables observed were actinobacteria characteristics and biosafety test. 2.) Selection of indigenous actinobacteria to suppress the growth of fungus F. verticillioides. The observed variable is the percentage of inhibition. 3.) The ability of actinobacteria in controlling cob rot in corn plants with 12 treatments and 3 replications, 10 isolates (selection results of stage II), 1 positive control, and 1 negative control, arranged in a completely randomized design. The variables observed were disease development and plant growth. A total of 20 isolates of actinobacteria were obtained isolation results, and the results of biosafety tests obtained as many as 15 isolates of actinobacteria. Actinobacteria isolates that have the potential to suppress the growth of fungus F. verticillioides are actinobacterial isolates APPB BI7, APPB CS7, APPA BI6, APPA AS7, APBC AS7, APPB AS7, APBA AS7, ALKA AS7, APBB BI6, and ALKB AI7 with an inhibition of 62.22-68%. Actinobacteria isolates that have the potential in suppressing the development of cob rot disease and spurring the growth of corn corn plants are isolates with the code APPB BI7, APBB BI6, ALKB AI7, APPB CS7, APPB AS7, APPA AS7, APBA AS7, and APBC AS7.

ABSTRAK

Fusarium verticillioides merupakan jamur yang menyebabkan penyakit busuk tongkol pada tanaman jagung. Pengendalian Fusarium verticilliodes dengan menggunakan agensia hayati yang bersifat antagonis yaitu aktinobakteria. Penelitian bertujuan untuk mendapatkan isolat aktinobakteria yang dapat mengendalikan penyakit busuk tongkol serta meningkatkan pertumbuhan jagung. Penelitian terdiri atas 3 tahap, 1.) Isolasi aktinobakteria indigenus dan F.verticillioides. Variabel yang diamati adalah karakteristik aktinobakteria dan uji keamanan hayati. 2.) Seleksi aktinobakteria indigenus untuk menekan pertumbuhan jamur F. verticillioides. Variabel yang diamati adalah persentase daya hambat. 3.) Kemampuan aktinobakteria dalam mengendalikan busuk tongkol pada tanaman jagung dengan 12 perlakuan dan 3 ulangan, 10 isolat (hasil seleksi tahap I dan II), 1 kontrol positif, dan 1 kontrol negatif, disusun dengan Rancangan Acak Lengkap. Variabel yang diamati adalah perkembangan penyakit dan pertumbuhan tanaman. Diperoleh 20 isolat aktinobakteria hasil isolasi, dan hasil uji keamanan hayati diperoleh sebanyak 15 isolat aktinobakteria. Isolat aktinobakteria yang berpotensi dalam menekan pertumbuhan jamur F. verticillioides yaitu isolat aktinobakteria APPB BI7, APPB CS7, APPA BI6, APPA AS7, APBC AS7, APPB AS7, APBA AS7, ALKA AS7, APBB BI6, dan ALKB AI7 dengan daya hambat 62,22-68,06%. Isolat aktinobakteria yang berpotensi dalam menekan perkembangan penyakit busuk tongkol dan memacu pertumbuhan tanaman jagung adalah isolat dengan kode APPB BI7, APBB BI6, ALKB AI7, APPB CS7, APPB AS7, APPA AS7, APBA AS7, dan APBC AS7.

Keywords


Actinobacteria, cop rot, corn, Fusarium verticillioides

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DOI: https://doi.org/10.15575/39831

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