The Effect of Extraction Method on the Extract Yield in the Carotenoid Pigment Encapsulation for Halal Natural Pigment

Imelda Fajriati; Atika Yahdiyani Ikhsani; Annisa Monitasari; Muhammad Zamhari; Betania Kartika; Jas Raj Subba

doi:10.15575/ijhar.v4i2.17188

Authors

Imelda Fajriati Universitas Islam Negeri Sunan Kalijaga, Indonesia
Atika Yahdiyani Ikhsani Universitas Islam Negeri Sunan Kalijaga, Indonesia
Annisa Monitasari Universitas Islam Negeri Sunan Kalijaga, Indonesia
Muhammad Zamhari Universitas Islam Negeri Sunan Kalijaga, Indonesia
Betania Kartika International Institute for Halal Research and Training (INHART), International Islamic University Malaysia (IIUM), Malaysia
Jas Raj Subba Royal University of Bhutan, Bhutan

DOI:

https://doi.org/10.15575/ijhar.v4i2.17188

Keywords:

caretonoid, encapsulation, extraction, halal natural pigment, maceration, Soxhlet

Abstract

The Soxhlet and maceration methods were used to determine the extract yield in the carotenoid pigment encapsulation for halal natural pigment production. This study aims to obtain halal natural pigment by determining the highest extract yield from the encapsulation of β-carotene in carrots. The carrot was extracted using Soxhlet and maceration method and then continued by oven drying. The n-hexane was selected because of its better volatility than ethanol and provided less solvent residue after extraction. UV-Vis spectroscopy and Thin Layer Chromatography (TLC) were used to characterize the n-hexane yield extract. Encapsulation of the pigment was investigated by adding five grams of maltodextrin to extract n-hexane weights of 0.05, 0.50, 0.75, and 1.0 grams. The maceration method yielded a much higher yield than the Soxhlet extraction method, with 2.24% (w/w) and 0.88% (w/w), respectively. The n-hexane extract absorbed a maximum wavelength of 450 nm with a retention factor (R_f) of 0.62. These values are confirmed by comparing the band's Rf values and absorption spectra with the standard's. Light absorption spectra at wavelengths 350-500 nm confirmed an intense color expression for encapsulation containing the highest pigment concentration.

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