Preliminary Immunochemical Studies to Detect Lard

Authors

  • Dina Sudjana Halal Center Salman ITB
  • Lia Amalia Universitas Djuanda Bogor, Indonesia
  • Saepul Adnan Universitas Muhammadiyah Bandung, Indonesia

DOI:

https://doi.org/10.15575/ijhar.v2i1.7819

Keywords:

antibody, antigen, immunochemical, lard

Abstract

Detection of lard in food using immunochemical methods has been carried out. This method has been applied and developed in food analysis. The purpose of this study was to detect the presence of lard in food. The method used was immunochemical which in this case is tested to its application in food analysis. The principle method is based on the antigen-antibody reaction, between lipids as antigens that can be deposited by antibodies in the agar medium. Antibodies were obtained by inducing lard into the blood vessels of rabbits. This method is based on the antigen-antibody reaction between fat as an antigen and antibodies that contain anti-lard as reagents and the occurrence of precipitation in agar media. The antibody was obtained by inducing lard, pork broth, and pig plasma into the blood vessels of rabbits. The results of this study were significant.

References

Allen J. C. & C. J Smith. (1987). Enzyme Linked Imunoassay Kit for Routine Food Analysis. Tibtec, 5.

Chen J. X. F. (2009). A Novel Quantum Dot-Based Fluoroimmunoassay Method for Detection Of ENR Residue in Chicken. Food Chemistry.

Citrasari, D. 2015. Penentuan Adulterasi Daging Babi Pada Pada Nugget AyamMenggunakan NIR dan Kemometrik. Skripsi. Fakultasi Farmasi. Universitas Jember.

Djatmiko, B & Wijaya, A. P. (1974). Minyak dan Lemak Department Teknologi Hasil Pertanian. Fateta. IPB.

Ditjen POM, Depskes RI., 1979. Farmakope Indonesia ed 3, Depkes RI, Jakarta.

Halal MUI. (2014). http://www.halalmui.org/newMUI/ diakses pada 20 Februari 2014.

Hiilditch T. P. (1979). Chemical Contituen of Natural Fats, 2nded. Chapman and Hill Ltd: London.

Hilda, L. (2014), Analisis Kandungan Lemak Babi dalam Produk Pangan di Padang Sidimpuan Secara Kualitatif dengan Menggunakan Gas Kromatografi. Tesis. Padang Sidimpuan.

Garvey, J. S. (1977). Metode in Imunologi, 3rd, ed Addison. Weshley publ Co. Inc.

Gatra. (2015). Melindungi Umat melalui Penelitian. Gatra Edisi Lebaran (Bisnis Halal Gaya Hidup Halal).

Kabat, E. A. (1976). Structure Concept In Imunology And Immunochemestry, New York, Chicago.

Kimbal, J. W. (1978). Introduction to Imunology 2nded. New York: Macmillan publ Co.

Kataren, S. (1986). Minyak dan Lemak Pangan. Jakarta: UI press.

Kisman, S. (1989). Kimia Analisis Bahan Makanan. Program Study Teknologi Pangan UNPAS.

Meyer, L. H. (1979). Fat and Other Lipidin Food Chemistry. Berlin: Springer.

Nowothy, A. (1979). Basic Exersises in Imunochemistry a Laboratorium. Berlin.

Rohman, A. (2013). Analisis Komponen Makanan. Yogyakarta: Graha Ilmu.

Warr, G. (1984). Antibody as a Tool. New York: John Willey and Son.

Downloads

Published

2020-03-01

How to Cite

Sudjana, D., Amalia, L., & Adnan, S. (2020). Preliminary Immunochemical Studies to Detect Lard. Indonesian Journal of Halal Research, 2(1), 8–12. https://doi.org/10.15575/ijhar.v2i1.7819

Issue

Section

Articles

Citation Check