Sus sp. DNA Encoding cyt b Gene Detection Test on Meat Grinding Samples Using Conventional PCR


Miftahul Lathif Adzakiyyi(1), Tri Susilowati(2), Saiku Rokhim(3), Yuanita Rachmawati(4*)

(1) Department of Biology, Faculty of Science and Technology, UIN Sunan Ampel Surabaya, Indonesia
(2) Department of Biology, Faculty of Science and Technology, UIN Sunan Ampel Surabaya, Indonesia
(3) Department of Biology, Faculty of Science and Technology, UIN Sunan Ampel Surabaya, Indonesia
(4) Department of Science Education, Faculty of Tarbiyah, UIN Sunan Ampel Surabaya, Indonesia
(*) Corresponding Author

Abstract


Micro-entrepreneurs with basic ingredients of processed meat such as meatball who do not have a meat grinder, generally using meat grinder at the public market. The problem that occurs is that there is no clear regulation from the Government regarding the guarantee of the halal meat grinding in the Regional Company. This needs to be enhanced as a study, considering that the grinding material does not only come from Halal substances. The purpose of this study was to test pig DNA in meat grinding samples at PD Pasar Surya Surabaya City by using the conventional Polymerase Chain Reaction (PCR) method. DNA was isolated from 11 PD Pasar Surya meat grinding samples, then spectrophotometry was performed. Spectrophotometry results showed that all samples have high DNA concentrations. The primer used is the cyt b pig gene encoder. Predenaturation is performed at a temperature of 95°C-5 minutes, denaturation of 95°C-45 seconds, annealing 60°C-30 seconds, extension 72°C-40 seconds, and post extension 72°C-5 minutes. The results of PCR analysis were determined by the emergence of DNA bands of ± 149 bp as markers of pig DNA. The results showed negative on sample or no pig contamination in 11 samples tested. While the pig sample as positive control showed a band of ± 149 bp. These results prove that at 11 points of the location of meat grinding there is no contamination of pig DNA.

Keywords


conventional PCR; Sus sp. cyt b gene; meat grinding

Full Text:

PDF

References


Baihaqi, M., Rachmawati, Y., Rokhim, S., Munir, M., & Hamidah, L. (2019). Real Time PCR Assays for Detection and Quantification of Porcine DNA in Meat Milling Samples. ICOST: EAI Proceeding, 1–7. https://doi.org/10.4108/eai.2-5-2019.2284634

Dooley, J. J., Paine, K. E., Garrett, S. D., & Brown, H. M. (2004). Detection of Meat Species using TaqMan Real-time PCR Assays. Meat Science, 68(3), 431–438. https://doi.org/https://doi.org/10.1016/j.meatsci.2004.04.010

Fatchiyah, A. E. L., Widyarti, S., & Rahayu, S. (2011). Biologi Molekular Prinsip Dasar Analisis. Jakarta: Erlangga.

Jain, S., Brahmbhatt, M. N., Rank, D. N., Joshi, C., & Solanki, J. V. (2007). Use of Cytochrome b Gene Variability in Detecting Meat Species by Multiplex PCR Assay. Indian Journal of Animal Sciences, 77(9), 880–881.

Muladno. (2010). Teknologi Rekayasa Genetik (2nd ed.). Bogor: IPB Press.

Pahlevi, M. R. (2013). Deteksi Cemaran Babi dengan Porcine Detection Kits Penggilingan Bakso di Kota Bogor. Institut Pertanian Bogor.

Peccia, J., & Hernandez, M. (2006). Incorporating Polymerase Chain Reaction-Based Identification, Population Characterization, and Quantification of Microorganisms Into Aerosol Science: A Review. Atmospheric Environment, 40, 3941–3961.

Putra, S. T. (1997). Biologi Molekuler Kedokteran. Surabaya: Airlangga University Press.

Rachmawati, Y., & Daryono, B. S. (2014). Karakter Fenotip dan Molekular Melon (Cucumis melo L.” Tacapa”) pada Media Tanam Tanah Karst. Yogyakarta: Universitas Gadjah Mada.

Rachmawati, Y., Rokhim, S., Munir, M., & Agustina, E. (2018). Deteksi Kontaminan Fragmen DNA Pengkode cyt b Babi Pada Sampel SOFTGELLCANDY Tak Berlabel Halal. Indonesia Journal of Halal, 1(1), 25. https://doi.org/10.14710/halal.v1i1.3115

Septiani, T. (2019). Detection of Porcine DNA in Processed Beef Products Using Real Time – Polymerase Chain Reaction. Indonesian Journal of Halal Research, 1(2), 31–34. https://doi.org/10.15575/ijhar.v1i2.5601

Widayanti, R., Solihin, D., Sajuthi, D., & Perwitasari, R. (2006). Kajian penanda genetik gen cytochrome B pada Tarsius sp. Jurnal Sain Veteriner, 24(1), 1–8.

Yusuf, Z. K. (2010). Polymerase Chain Reaction (PCR). Saintek, 5(6), 1–6. https://doi.org/10.11501/3061795




DOI: https://doi.org/10.15575/ijhar.v2i2.8489

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Journal of Halal Research Indexed By:

          


Published By:

Halal Center

UIN Sunan Gunung Djati

Gedung Solahuddin Sanusi (Laboratorium Terpadu)

Jl. A.H. Nasution No. 105, Cibiru, Bandung, West Java 40614 - Indonesia 

Creative Commons License
Indonesian Journal of Halal Research by Halal Center UIN Sunan Gunung Djati Bandung is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Based on a work at https://journal.uinsgd.ac.id/index.php/ijhar.