Chemical Composition and Structural Characterization of a Basil (Ocimum basilicum L.) Leaf Extract–Bionutrient S-367B Composite
DOI:
https://doi.org/10.15575/jtk.v11i1.54888Keywords:
basil leaf extract, bionutrient S-367B, biopesticide, broccoli growth, sustainable agricultureAbstract
The excessive use of synthetic pesticides in horticultural production poses serious environmental and health concerns, necessitating the development of eco-friendly alternatives. This study evaluates the effectiveness of a composite biopesticide formulated from basil leaf (Ocimum basilicum L.) extract and Bionutrient S-367B on the functional response and yield of broccoli (Brassica oleracea var. italica). The extract was composited with Bionutrient S-367B at concentrations of 25%, 50%, and 75% and applied at dosages of 5, 7.5, and 10 mL/L. Phytochemical analysis confirmed the presence of alkaloids, flavonoids, saponins, and tannins, with a total phenolic content of 27.624 ± 1.468 mg GAE/g. FTIR characterization revealed key functional groups, including hydroxyl (–OH), aliphatic C–H, carbonyl (C=O), aromatic C=C, and C–N groups, indicating the presence of phenolic and non-phenolic bioactive compounds. The composite treatment showed improved functional response and yield of broccoli, particularly at a 50% concentration with a dosage of 7.5 mL/L, which produced the highest plant height, leaf dimensions, growth rate constant, and harvested biomass. However, statistical analysis indicated that these differences were not significant at the 95% confidence level (P > 0.05) across most measured parameters. These findings suggest that the integration of plant-based biopesticides with bionutrients has the potential to enhance crop performance while maintaining environmental sustainability. This study provides an integrated chemical and agronomic perspective on the application of basil leaf extract–Bionutrient S-367B composites as a promising eco-friendly strategy for sustainable broccoli production.
References
Akude, D. N., Akuma, J. K., Kwaning, E. A., & Avevor, P. B. (2024). Green Brand Communication and the Profitability of Manufacturing Firms: The Moderating Role of Environmental Commitment. Multidisciplinary Reviews, 8(4). https://doi.org/10.31893/multirev.2025125
Azwanida, N. N. (2015). A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal & Aromatic Plants, 4(3), 196. https://doi.org/10.4172/2167-0412.1000196
Babayan, A. M. (2023). Antimicrobial and Antioxidant Activities of Ocimum basilicum var. purpureum ethanol extract. Chemistry and Biology, 258–268. https://doi.org/10.46991/PYSU
Bahri, S., Wirdullutfi, W., & Hijriani, B. I. (2025). Qualitative Phytochemical Screening of Ethanolic Extract of Ocimum basilicum L. Leaves. Jurnal Biologi Tropis, 25(4a), 782–785. https://doi.org/10.29303/jbt.v25i4a.11142
Barad, A. H., Prajapati, H. N., Parekh, D. D., & Trivedi, N. P. (2024). Efficacy of Organic Inputs Against Aphid, Lipaphis pseudobrassicae Davis Infesting Broccoli. International Journal of Advanced Biochemistry Research, 8(7), 92–95. https://doi.org/10.33545/26174693.2024.v8.i7b.1449
Bernhardt, B., Sipos, L., Kókai, Z., Gere, A., Szabó, K., Bernáth, J., & Sárosi, S. (2015). Comparison of Different Ocimum basilicum L. Gene Bank Accessions Analyzed by GC–MS and Sensory Profile. Industrial Crops and Products, 67, 498–508. https://doi.org/10.1016/j.indcrop.2015.01.013
Damanik, M. U. (2023). Kombinasi ekstrak sambiloto (Andrographis paniculate Nees.) dan bionutrien S-367B yang diaplikasikan pada tanaman brokoli (Brassica oleraceae L.) [Undergraduate thesis, Universitas Pendidikan Indonesia]. Retrieved from https://repository.upi.edu/103127/
Dharani, E., Shricharan, S., Balaji, B., & Yasin, J. K. (2023). Nutritional Revolution: Biotechnological Strategies for Fortified Crop Produce (pp. 355–371). Apple Academic. https://doi.org/10.1201/9781003402084-16
Diba, F., Nauli, U. R., Winarsih, W., & Oramahi, H. A. (2022). The Potency of Ocimum basilicum Leaf as Biopesticide. Journal of Tropical Biology, 22(1), 304–314. https://doi.org/10.29303/jbt.v22i1.3023
Dvořáčková, H., Dvořáček, J., González, P. H., & Vlček, V. (2022). Effect of Different Soil Amendments on Soil Buffering Capacity. PLoS ONE, 17(2), e0263456. https://doi.org/10.1371/journal.pone.0263456
Humane, A. N., Undirwade, D. B., Borkar, S. L., Sawai, H. R., Lawhe, N. V., & Chaudhari, B. N. (2023). Pesticide-Induced Stress and Innovative Strategies for Plant Resilience and Environmental Health (pp. 21–36). Iterative. https://doi.org/10.58532/v3bcag10p1ch2
Jote, C. A. (2023). The Impacts of Using Inorganic Chemical Fertilizers on the Environment and Human Health. Organic and Medicinal Chemistry International Journal, 13(3). https://doi.org/10.19080/OMCIJ.2023.13.555864
Juliana J, Limayurid AS, Adirestuty F, Ridlwan AA, Rusmita SA, Ismail S (2025), Intention to Buy Halal Food through the ShopeeFood Application on Generation Z Muslims. Journal of Islamic Accounting and Business Research, Vol. 16 No. 8 pp. 1349–1369, doi: https://doi.org/10.1108/JIABR-04-2023-0120
Kashyap, D., de Vries, M., Pronk, A., & Adiyoga, W. (2023). Environmental Impact Assessment of Vegetable Production in West Java, Indonesia. Science of the Total Environment, 864. https://doi.org/10.1016/j.scitotenv.2022.160999
Klatt, B. K., Wurz, A., Herbertsson, L., Rundlöf, M., Svensson, G. P., Kuhn, J., Vessling, S., De La Vega, B., Tscharntke, T., Clough, Y., & Smith, H. G. (2023). Seed Treatment with Clothianidin Induces Changes in Plant Metabolism and Alters Pollinator Foraging Preferences. Ecotoxicology, 32(10), 1247–1256. https://doi.org/10.1007/s10646-023-02720-0
Korzin, V., Tsiupka, S., Plugatar, Y., Tsiupka, V., Shoferistov, E., & Korzh, D. (2022). Photosynthetic Activity of Olive Leaves Before and After Treatment with an Experimental Mixture of Pesticides. Acta Horticulturae, 1339, 377–382. https://doi.org/10.17660/actahortic.2022.1339.47
Lippens, L., Fleerakkers, S., Temmerman, F., & De Roissart, A. (2022). Practical application of integrated pest management to control cabbage root fly in vegetables. In Achieving sustainable cultivation of vegetables (pp. 421–456). Burleigh Dodds Science Publishing. https://doi.org/10.19103/AS.2021.0095.16
Madani, R. F. (2023). Kombinasi Ekstrak Daun Mimba (Azadirachta indica) dan Bionutrien S-367B yang diaplikasikan pada Tanaman Brokoli (Brassica oleracea) [Undergraduate thesis, Universitas Pendidikan Indonesia]. Retrieved from https://repository.upi.edu/103997/
Monib, A. W., Niazi, P., & Sediqi, S. (2023). Investigating Approaches for Optimizing Agricultural Yield: A Comprehensive Review of the Crucial Role of Micronutrients in Enhancing Plant Growth and Maximizing Production. Journal for Research in Applied Sciences and Biotechnology, 2(5), 168–180. https://doi.org/10.55544/jrasb.2.5.26
Nagi, K., Neog, P., & Bala, B. (2024). Efficacy of Certain Bio-Pesticides against the Major Insect Pests of Broccoli (Brassica oleracea var. italica L.). Environment and Ecology, 42(4), 1504–1509. https://doi.org/10.60151/envec/lscg3609
Nagraj, G. S., Chouksey, A., Jaiswal, S., & Jaiswal, A. K. (2020). Broccoli. In Nutritional Composition and Antioxidant Properties of Fruits and Vegetables (pp. 5–17). https://doi.org/10.1016/B978-0-12-812780-3.00001-5
Panchal, A., & Maitreya, B. (2023). A Review on Exploring the Significance of Micronutrients in Crop Production. International Association of Biologicals and Computational Digest, 2(2), 51–58. https://doi.org/10.56588/iabcd.v2i2.183
Ramya, S. K., & Kumar, S. S. (2024). Significance of Micro-irrigation for Feasible and Sustainable Agriculture: A Review. Multidisciplinary Reviews, 8(4), 2025094. https://doi.org/10.31893/multirev.2025094
Rys, M., Saja-Garbarz, D., & Skoczowski, A. (2022). Phytotoxic Effects of Selected Herbal Extracts on the Germination, Growth and Metabolism of Mustard and Oilseed Rape. Agronomy, 12(1), 110. https://doi.org/10.3390/agronomy12010110
Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., & Yoga Latha, L. (2011). Extraction, Isolation and Characterization of Bioactive Compounds from Plants’ Extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8(1), 1–10. https://doi.org/10.4314/ajtcam.v8i1.60483
Son, D., Bonzi, S., Somda, I., Legreve, A., & Schiffers, B. (2018). Efficacy of Ocimum basilicum L. Extracts against tomato wilt (Fusarium oxysporum f. sp. radicis-lycopersici) in Burkina Faso. Communications in Applied Biological Sciences, 83(2). Retrieved from https://academicjournals.org/journal/AJPS/article-references/D4FE33B71484
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