Penapisan isolat rizobakteri indigenos untuk pengendalian (Ganoderma boninense) di pre nursery kelapa sawit (Elaeis guineensis Jacq.)


yulmira yanti(1*), Imam Rifai(2), Yogie Aditya Pratama(3), Muhammad Ihsan Harahap(4)

(1) andalas university padang, Indonesia
(2) ,  
(3) ,  
(4) ,  
(*) Corresponding Author

Abstract


Rizobakteri merupakan kelompok bakteri yang aktif mengkolonisasi akar tanaman, meningkatkan pertumbuhan dan mengendalikan patogen tanaman. Penelitian ini bertujuan untuk memperoleh isolat rizobakteri indigenous  terbaik dalam meningkatkan pertumbuhan kelapa sawit dan mengendalikan penyakit busuk pangkal batang di pre-nursery kelapa sawit secara in planta serta karakterisasi kemampuan antagonisnya secara in vitro. Penelitian bersifat eksperimental terdiri atas 3 tahap dengan menggunakan Rancangan Acak Lengkap (RAL): (1) Isolasi dan karakterisasi isolat rizobakteri indigenous  di Kabupaten Pasaman Barat, (2) Pengujian isolat rizobakteri indigenous  (RBI) sebagai plant growth promoting rihzobacteria (PGPR), dan untuk pengendalian G.boninense di pre-nursery kelapa sawit terdiri dari 29 perlakuan (27 isolat RBI, tanpa inokulasi G. boninense sebagai kontrol positif, dan inokulasi G. boninense sebagai kontrol negatif) dengan masing-masing 5 ulangan, serta (3) Pengujian aktivitas antagonisme isolat RBI terhadap G. boninense. Data dianalisis dengan sidik ragam, apabila berbeda nyata dilanjutkan dengan uji Least Significance Different (LSD) pada taraf 5%. Hasil penelitian menunjukkan diperoleh tiga isolat terbaik (R10 2.2, R9 2.1, dan R10 2.3) yang mampu meningkatkan pertumbuhan kelapa sawit dan menekan perkembangan penyakit busuk pangkal batang G.boninense secara in planta dan in vitro.

ABSTRACT

Rhizobacteria is a group of bacteria that actively colonize plant roots, increase growth and control plant pathogen. The objective of the research was to obtain indigenous rhizobacteria isolate (RBI) to increase growth and control basal stem rot on oil palm seedlings in in planta and characterize of antagonistic ability in in vitro. Experimental research consisted of 3 stages by using Completely Randomized Design (CRD): (1) Isolation of indigenous rhizobacteria in West Pasaman region, (2) Indigenous rhizobacteria isolate testing as a plant growth promoting rhizobacteria (PGPR) and to control of G. boninense on pre nursery of oil palm consisted of 29 treatments (27 RBI isolates, without G. boninense inoculation as positive control, and G. boninense inoculation as negative control) with 5 replications each. (3) Testing of RBI isolate antagonism activity towards G. boninense. Data were analyzed by variance, if the result significantly different, it was continued by using Least Significance Different (LSD) at 5% level. The results showed that best three isolates (R10 2.2, R9 2.1 and R10 2.3) were able to increase growth of palm oil and to suppress the development of G.boninense basal stem rot in in planta and in in vitro.


Keywords


Antagonis, in planta, in vitro, kelapa sawit, rizobakteri

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References


Bakhtiar, Y., Yahya, S., & Sumaryono, W. (2012). Adaptation of oil palm seedlings inoculated with arbuscular mycorrhizal fungi and mycorrhizal endosymbiotic bacteria Bacillus subtilis B10 towards biotic stress of pathogen Ganoderma boninense Pat. Jurnal Microbiology Indonesia, 6(4), 157–164. https://doi.org/10.5454/mi.6.4.3

Beneduzi, A., Ambrosini, A., & Passaglia, L. M. P. (2012). Plant growth-promoting rhizobacteria ( PGPR ): Their potential as antagonists and biocontrol agents. Genetics and Molecular Biology, 4, 1044–1051.

Bhattarai, T. (1993). Yield responses of Nepalese spring wheat (Triticum aestivum L.) cultivars to inoculation with Azospirillum spp. of Nepalese origin. Plant and Soil, 67–68.

Bivi, M. R., Farhana, M. S., Khairulmazmi, A., & Idris, A. (2010). Control of ganoderma boninense: A causal agent of basal stem rot disease in oil palm with endophyte bacteria in vitro. International Journal of Agriculture and Biology, 12(6), 833–839.

Buana, RFN, Wahyudi AT, T.-M. N. (2014). Buana, RFN, Wahyudi AT, Toruan-Mathius N. 2014. Control activity of potential antifungal-producing Burkholderia sp. in suppressing Ganoderma growth in oil palm. A. J Agriculture Res., 8(5):259-268.

Chong, K. P., Lum, M. S., Foong, C. P., Wong, C. M. V. L., Atong, M., & Rossall, S. (2011). First identification of Ganoderma boninense isolated from Sabah based on PCR and sequence homology. African Journal of Biotechnology, 10(66), 14718–14723. https://doi.org/10.5897/AJB11.1096

Direktorat Jenderal Perkebunan. (2017). Outlook kelapa sawit komoditas pertanian subsektor perkebunan. Pusat data informasi pertanian. Sekretariat Jenderal-Kementrian Pertanian.

Fauzi, Y., Y.S. Widyastuti, I. Setyawibawa, dan R.H. Paeru. 2012. Kelapa Sawit. Penebar Swadaya. Jakarta. 234 pp

Li, F. X., Ma, H. Q., Liu, J., & Zhang, C. (2012). Antagonistic effects of Bacillus cereus strain B-02 on morphology, ultrastructure and cytophysiology of Botrytis cinerea. Polish Journal of Microbiology, 61(2), 119–128.

Mohd, Z., & Faridah, A. (2008). Disease Suppression in Ganoderma -infected Oil Palm Seedlings Treated with Trichoderma harzianum. Plant Protection Science, 44(3), 101–107.

Neeraja, C., Anil, K., Purushotham, P., Suma, K., Sarma, PVSRN., Moerschbacher, B. M., & Podile, A. R. (2010). Biotechnological approaches to develop bacterial chitinases as a bioshield against fungal diseases of plants. Critical Reviews in Biotechnology; 30(3): 231–241.

Ommelna, B.G., Jennifer, A.N., Chong, K.P. (2012). The potential of chitosan in suppressing Ganoderma boninense infection in oil-palm seedlings. J Sustain Sci Manage. 7(2):186–192.

Puspita, F, Zul D, K. A. (2013). Potensi Bacillus sp. Asal Rizosfer Giam Siak Kecil Bukit Batu Sebagai Rhizobacteria Pemacu Pertumbuhan dan Antifungi pada Pembibitan Kelapa Sawit. Seminar Nasional “Peranan Teknologi Dan Kelembagaan Pertanian Dalam Mewujudkan Pembangunan Pertanian Yang Tangguh Dan Berkelanjutan, 2009(November), 7–15.

R, A. K., Ayu, G., Sutariati, K., & Rahman, A. (2011). Potensi rizobakteri indigenous Ultisol untuk mengendalikan penyakit busuk batang Phytophthora (Phytophthora capsici ) pada tanaman cabai. Jurnal Agroteknos, 1(1), 8–13.

Rashid, M., Rakib, M., Bong, C. J., Khairulmazmi, A., & Idris, A. S. (2014). Genetic and morphological diversity of Ganoderma species isolated from infected oil palms (Elaeis guineensis). International Journal of Agriculture & Biology, 16(4), 691–699.

Rees, R. W., Flood, J., Hasan, Y., Wills, M. A., & Cooper, R. M. (2012). Ganoderma boninense basidiospores in oil palm plantations: Evaluation of their possible role in stem rots of Elaeis guineensis. Plant Pathology, 61(3), 567–578. https://doi.org/10.1111/j.1365-3059.2011.02533.x

Suryanto, D, Wibowo RH, Siregar EBM, M. E. (2012). A possibility of chitinolytic bacteria utilization to control basal stems disease caused by Ganoderma boninense in oil palm seedling. African Journal of Microbiology Research, 6(9), 2053–2059.

https://doi.org/10.5897/ajmr11.1343

Susanto, A., Prasetyo, A., & Wening, S. (2014). Laju infeksi ganoderma pada empat kelas tekstur tanah. Jurnal Fitopatologi Indonesia, 9(2), 39–46. https://doi.org/10.14692/jfi.9.2.39

Sutariati, G. A. K., Rakian, T. C., Sopacua, N., & Mudi, L., & Haq, M. (2014). Kajian potensi rizobakteri pemacu pertumbuhan tanaman yang diisolasi dari rizosfer padi sehat. Jurnal Agroteknos, 4(2), 71–77.

Verma, J.P., Yadav J., Tiwari K.N., Lavakush, & singh V. (2010). Impact of plant growth promoting rhizobacteria on crop production. International Journal of Agriculture Research, 5(11), 954–983.

Vishwakarma, K., Kumar, V., Tripathi, D. K., & Sharma, S. (2018). Characterization of rhizobacterial isolates from Brassica juncea for multitrait plant growth promotion and their viability studies on carriers. Environmental Sustainability, 1(3), 253–265. https://doi.org/10.1007/s42398-018-0026-y.

Wibowo, R. H., Mubarik, N. R., Rusmana, I., & Thenawidjaya, M. (2017). Penapisan dan Identifikasi bakteri kitinolitik penghambat pertumbuhan Ganoderma boninense in vitro. Jurnal Fitopatologi Indonesia, 13(3), 105–111. https://doi.org/10.14692/jfi.13.3.105

Yanti, Y, Astuti FF, Habazar T, N. C. R. (2017). Screening of rhizobacteria from rhizosphere of healthy chili to control bacterial wilt disease and to promote growth and yield of chili. Jurnal Biodiversitas, 18(1), 1–9. https://doi.org/10.13057/biodiv/d180101

Yanti, Y., Habazar, T., Resti, Z., & Suhalita, D. (2013). Penapisan isolat rizobakteri dari perakaran tanaman kedelai yang sehat untuk pengendalian penyakit pustul bakteri (Xanthomonas axonopodis Pv. Glycines). J. HPT Tropika., 13(1), 24–34.

Zahid, M., Abbasi, M K., Rahim, N. (2015). Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L .). 6(March), 1–10. https://doi.org/10.3389/fmicb.2015.00207.




DOI: https://doi.org/10.15575/4665

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