Efek inokulasi PGPR terhadap pertumbuhan tanaman padi fase vegetative di media salinitas tinggi

Eka Junianti, Elly Proklamasiningsih, Purwanto Purwanto

Abstract


Rhizobakteria merupakan kelompok bakteri yang hidup di perakaran tanaman yang bermanfaat bagi pertumbuhan tanaman melalui kemampuan menghasilkan IAA. Rhizobakteria dapat hidup di berbagai kondisi lingkungan, salah satunya di lahan salin. Tujuan penelitian ini untuk mengkaji efek Plant-Growth Promoting Rhizobacteria bagi tanaman padi pada fase vegetatif di media dengan silinitas tinggi, dan mendapatkan isolat yang paling efektif meningkatkan pertumbuhan vegetatif padi varietas Inpari Unsoed 79 Agritan. Penelitian ini dilakukan di Laboratorium Agronomi dan Hortikultura, dan Screenhouse Experimental Farm Fakultas Pertanian UNSOED pada bulan Oktober 2019-Januari 2020. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok Lengkap dengan 6 perlakuan dan diulang 4 kali. Perlakuan pada penelitian ini antara lain kontrol, JA2, JB1, JB2, JD1, dan JE1 pada medium cair AB Mix dengan tingkat salinitas 5 dSm-1. Inokulasi PGPR meningkatkan pertumbuhan tanaman padi pada fase vegetatif. Bobot kering tanaman, panjang akar, kehijauan daun, luas daun, tinggi tanaman masing-masing meningkat sebesar 130%, 108%, 19%, 50%, 21% dengan inokulasi PGPR pada medium dengan salinitas tinggi.  

 

Rhizobacteria are a group of bacteria that live in rhizozphere of plant which are beneficial for plant growth through the ability to produce IAA. Rhizobacteria can live in various environmental conditions, one of which is in saline fields. The research goal was to study the effect of PGPR for rice plants in the vegetative phase in high salinity media and to find the most effective isolates in increasing vegetative growth of Inpari Unsoed 79 Agritan rice. This research was conducted at the Agronomy and Horticulture Laboratory, and the screenhouse of Experimental Farm, Faculty of Agriculture, UNSOED in October 2019-January 2020. This research was arranged by using a Randomized Complete Block Design and repeated 4 times. The treatments in this study included control, JA2, JB1, JB2, JD1, and JE1 in AB Mix liquid medium with a salinity level of 5 dSm-1. The inoculation of rhizobacteria can increase the rice plant growth at vegetative phase. The plant biomass, root length, leaf greenness, leaf area, plant height increased by 130%, 108%, 19%, 50%, 21% respectively by PGPR inoculation of medium with high salinity.


Keywords


Padi, pertumbuhan, PGPR, salin, vegetatif

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References


Anggarwulan, E. (2008). Physiological characters of kimpul (Xanthosoma sagittifolium (L.) Schott) in various of light intensity (shading) and water availability. Biodiversitas, Journal of Biological Diversity, 9(4), 264–268. https://doi.org/10.13057/biodiv/d090405

Arrosyid, H., & Sugito, Y. (2018). Respon enam varietas tanaman tebu ( Saccharum officinarum L .) pada kondisi lingkungan cekaman garam. Jurnal Produksi Tanaman, 6(4), 678–684.

Athallah, F.N.F., U. Dinar, B.N. Fitriatin, T. Simarmata. (2018). Synergism bioassay of selected indigenous halotolerant phosphorizobacteria from rice rhizosphere on saline soil ecosystem Synergism bioassay of selected indigenous halotolerant phosphorizobacteria from rice rhizosphere on saline soil ecosystem. IOP Conf. Ser.: Earth Environ. Sci, 205(012014). https://doi.org/10.1088/1755-1315/205/1/012014

Cahyaty, R.A.A., Aini, N. & Sumarni, T., (2017). Pengaruh salinitas dan aplikasi bakteri rhizosfer toleran salin terhadap komponen hasil tanaman mentimun. Biotropika - Journal of Tropical Biology, 5(3), 133–137. https://doi.org/10.21776/ub.biotropika.2017.005.03.12

Dewi, T. K. (2015). Karakterisasi mikroba perakaran (PGPR) agen penting pendukung pupuk organik hayati. In PROS SEM NAS MASY BIODIV INDON. https://doi.org/10.13057/psnmbi/m010220

Fardhani, A.A., Simanjutak, D.I.N. & Wanto, A. (2018). Prediksi harga eceran beras di pasar tradisional di 33 kota di indonesia menggunakan algoritma backpropagation. Jurnal Infomedia, 3(1). https://doi.org/10.30811/jim.v3i1.625

Fauzan, I.A. (2018). Keragaman bakteri penghasil indole acetic acid (iaa) dari persawahan salin Pantai Jetis. Universitas Jenderal Soedirman.

Fitriatin, B.N., A. Yuniarti, O. Mulyanti, F.S Fauziah, & M.D. Tiara. (2009). Pengaruh mikroorganisme pelarut fosfat dan pupuk p terhadap p tersedia , aktivitas fosfatase , populasi mikroorganisme pelarut fosfat , konsentrasi p tanaman dan hasil padi gogo ( Oryza sativa . L .) pada Ultisols. Jurnal Agrikultura, 20(3), 210–215.

Gholami, A., Shahsavani, S., & Nezarat, S. (2009). The effect of plant growth promoting rhizobacteria ( PGPR ) on germination , seedling growth and yield of maize. World Academy of Science, Engineering and Technology, 19–24.

Habibi, S., Djedidi, S., Ohkama-Ohtsu, N., Sarhadi, W. A., Kojima, K., Rallos, R. V., Yokoyama, T. (2019). Isolation and screening of indigenous plant growth-promoting rhizobacteria from different rice cultivars in afghanistan soils. Microbes and Environments, 34(4), 347–355. https://doi.org/10.1264/jsme2.ME18168

Kurniahu, H., Sriwulan, S., & Andriani, R. (2018). Pemberian PGPR indigen untuk pertumbuhan kacang tanah (Arachis hypogaea L.) varietas lokal tuban pada media tanam bekas tambang kapur. Agrovigor: Jurnal Agroekoteknologi, 11(1), 52–57.

https://doi.org/10.21107/agrovigor.v11i1.4305

Kusumastuti, A. C., M. Kolopaking, L., & Barus, B. (2018). Factors affecting the converstion of agricultural land in Pandeglang Regency. Sodality: Jurnal Sosiologi Pedesaan, 6(2). https://doi.org/10.22500/sodality.v6i2.23234

Ma'ruf, A. (2016). Respon beberapa kultivar tanaman pangan terhadap salinitas. Bernas, 12(3), 11–19.

Mastur, (2016). Sinkronisasi source dan sink untuk peningkatan produktivitas biji pada tanaman jarak pagar. Buletin Tanaman Tembakau, Serat & Minyak Industri, 7(1), 52. https://doi.org/10.21082/bultas.v7n1.2015.52-68

Maulina, N.M.I., K. Khalimi, G.N.A.S. Wirya, D. N. S. (2015). Potensi rizobakteri yang diisolasi dari rizosfir tanaman graminae non-padi untuk memacu pertumbuhan bibit padi, J. Agric.Sci and Biotechmol 4(1), 1–8.

Maulina, N. M. I., & Darmayasa, I. D. N. (2018). Pemanfaatan rizobakteri isolat Al7Kla untuk memacu pertumbuhan. DwijenAGRO, 8(2), 134–143.

Nghia, N.K., T.T.M. Tien; N.T.K. Oanh, & N.H.K. Nuong. (2017). Isolation and Characterization of indole acetic acid producing halophilic bacteria from salt affected soil of rice–shrimp farming system in the Mekong Delta, Vietnam. Agriculture, Forestry and Fisheries, 6(3), 69. https://doi.org/10.11648/j.aff.20170603.11

Oktaviani, T.J., & M.D. Maghfoer. (2018). Respon pertumbuhan dan hasil tanaman pakcoy ( Brassica rapa L .) terhadap aplikasi EM dan PGPR. Jurnal Produksi Tanaman, 6(8), 1974–1981.

Rahni, N. M. (2012). Tanaman jagung ( Zea mays ). Jurnal Agribisnis Dan Pengembangan Wilayah, 3(16), 27–35.

Saharan, B. S., & Nehra, V. (2011). Plant growth promoting rhizobacteria: A Critical Review. Life Sciences and Medicine Research, 2011(1), 21.

Setiyowati, S., Haryanti, S., & Hastuti, R. B. (2012). Pengaruh perbedaan konsentrasi pupuk organik cair tehadap produksi bawang merah (Allium ascalonicum L). Bioma : Berkala Ilmiah Biologi, 12(2), 44. https://doi.org/10.14710/bioma.12.2.44-48

Shrivastava, P., & Kumar, R. (2015). Soil salinity : A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences, 22(2), 123–131. https://doi.org/10.1016/j.sjbs.2014.12.001

Sirait, J. (2008). Luas daun , kandungan klorofil dan laju pertumbuhan rumput pada naungan dan pemupukan yang berbeda. Jitv, 13(2), 109–116.

Subowo, Y. B. (2015). Penambahan pupuk hayati jamur sebagai pendukung pertumbuhan tanaman padi (Oryza sativa) pada tanah salin, PROS SEM NAS MASY BIODIV INDON 1(2007), 150–154. https://doi.org/10.13057/psnmbi/m010126

Widawati, S. S. (2016). Pengaruh salinitas dan inokulasi bakteri terhadap petumbuhan tanaman terung (Solanum melongena L.), Berita Biologi 15(1): 17-25

Yanti, Y., Hamid, H., & Reflin, R. (2019). Indigenous rhizobacteria screening from tomato to control Ralstonia syzigii subsp. indonesiensis and promote plant growth rate and yield. Jurnal Hama Dan Penyakit Tumbuhan Tropika, 18(2), 177. https://doi.org/10.23960/j.hptt.218177-185

Yuliani, D. Z. (2011). penggunaan agen hayati rhizobium sp. dan pseudomonas fluorescens terhadap pertumbuhan tanaman kedelai (Glycine max) pada tanah salin Retrieved from http://ejournal.unesa.ac.id/index.php/lenterabio

Zainudin, Abadi, A. L., & Aini, L. Q. (2014). Pengaruh pemberian Plant Growth Promoting Rhizobacteria (Bacillus subtilis dan Pseudomonas fluorescens)terhadap penyakit bulai pada tanaman jagung(Zea mays L.). Jurnal HPT, 2(1), 11–18.

Zerrouk, I. Z., Rahmoune, B., Khelifi, L., Mounir, K., Baluska, F., & Ludwig-Müller, J. (2019). Algerian Sahara PGPR confers maize root tolerance to salt and aluminum toxicity via ACC deaminase and IAA. Acta Physiologiae Plantarum, 41(6), 1–10. https://doi.org/10.1007/s11738-019-2881-2




DOI: https://doi.org/10.15575/8057

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